A5054624216- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Cancer Research and Treatments
- Proteoglycans and glycosaminoglycans research
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 and COVID-19 Research
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
- Advanced Proteomics Techniques and Applications
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- RNA and protein synthesis mechanisms
- Science, Research, and Medicine
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- Genetics, Aging, and Longevity in Model Organisms
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- Advanced Biosensing Techniques and Applications
- Cellular transport and secretion
- Glutathione Transferases and Polymorphisms
- Protease and Inhibitor Mechanisms
Institute of Molecular Biotechnology
2020-2024
Austrian Academy of Sciences
2020-2024
University of Michigan–Ann Arbor
2024
Medical University of Vienna
2024
University of Applied Sciences Upper Austria
2024
BOKU University
2024
University of British Columbia
2024
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2015-2021
Universidade do Porto
2015-2021
IPO Porto
2021
Article23 August 2021Open Access Transparent process Identification of lectin receptors for conserved SARS-CoV-2 glycosylation sites David Hoffmann IMBA, Institute Molecular Biotechnology the Austrian Academy Sciences, Vienna, Austria These authors contributed equally to this work Search more papers by author Stefan Mereiter orcid.org/0000-0002-4832-3090 Yoo Jin Oh orcid.org/0000-0002-9636-3329 Biophysics, Johannes Kepler University Linz, Vanessa Monteil orcid.org/0000-0002-2652-5695...
Article4 July 2022Open Access Transparent process Clinical grade ACE2 as a universal agent to block SARS-CoV-2 variants Vanessa Monteil orcid.org/0000-0002-2652-5695 Unit of Microbiology, Karolinska Institutet and University Hospital, Stockholm, Sweden Contribution: Conceptualization, Data curation, Formal analysis, Visualization, Methodology Search for more papers by this author Brett Eaton NIAID Integrated Research Facility at Fort Detrick (IRF-Frederick), Frederick, Maryland, USA analysis...
Abstract The clinical performance of the therapeutic monoclonal antibody trastuzumab in treatment ErbB2-positive unresectable gastric cancer (GC) is severely hampered by emergence molecular resistance. Trastuzumab’s target epitope localized within extracellular domain oncogenic cell surface receptor tyrosine kinase (RTK) ErbB2, which known to undergo extensive N -linked glycosylation. However, site-specific glycan repertoire as well detailed mechanisms through specific aberrant signatures...
The COVID-19 pandemic has demonstrated the need for massively-parallel, cost-effective tests monitoring viral spread. Here we present SARSeq, saliva analysis by RNA sequencing, a method to detect SARS-CoV-2 and other respiratory viruses on tens of thousands samples in parallel. SARSeq relies next generation sequencing multiple amplicons generated multiplexed RT-PCR reaction. Two-dimensional, unique dual indexing, using four indices per sample, enables unambiguous scalable assignment reads...
CD 44 isoforms are often upregulated in gastric cancer and have been associated with increased metastatic potential poor survival. To evaluate the functional impact of O‐glycan truncation on we analysed glyco‐engineered cell models displaying shortened O‐glycans. Here, demonstrate that induction aberrant termination through various molecular mechanisms affects features. We show is a major carrier truncated O‐glycans this accompanied by an hyaluronan binding capacity extracellular shedding....
Aberrant expression and hyperactivation of the human epidermal growth factor receptor 2 (ErbB2) constitute crucial molecular events underpinning gastric neoplastic transformation. Despite ErbB2 extracellular domain being a well-known target for glycosylation, its glycosylation profile mechanisms through which it actively tunes tumorigenesis in cancer (GC) cells remain elusive. We aimed at disclosing relevant glycan signatures their functional impact on receptor's biology GC cells. The...
Solute carrier (SLC) transporters govern most of the chemical exchange across cellular membranes and are integral to metabolic regulation, which in turn is linked function identity. Despite their key role, individual functions members SLC superfamily were not evaluated systematically. We determined transcriptional profiles upon overexpression knock-out or wild-type isogenic cell backgrounds. Targeted metabolomics provided a fingerprint 189 intracellular metabolites, while transcriptomics...
HACE1 is an E3 ubiquitin ligase with important roles in tumor biology and tissue homeostasis. Loss or mutation of
Modulation of immunity and disease by glycans is increasingly recognized. However, how host glycosylation shapes shaped tuberculosis remains poorly understood. We show that deficiency in the glucosaminyl (N-acetyl) transferase 1 (Gcnt1), a key enzyme for core-2 O-glycans biosynthesis, drives susceptibility to Mycobacterium infection. The increased Gcnt1 deficient mice was characterized extensive lung immune pathology, mechanistically related neutrophils. Uninfected presented bone marrow,...
N-glycosylation is one of the most common protein modifications in eukaryotes, with immense importance at molecular, cellular, and organismal level. Accurate reliable N-glycan analysis essential to obtain a systems-wide understanding fundamental biological processes. Due structural complexity glycans, their still highly challenging. Here we make publicly available consistent N-glycome dataset 20 different mouse tissues demonstrate multimodal data workflow that allows for unprecedented depth...
Cellular glycosylation plays a pivotal role in several molecular mechanisms controlling cell⁻cell recognition, communication, and adhesion. Thus, aberrant has major impact on the acquisition of malignant features tumor progression patients. To mimic these vivo features, an innovative high-throughput 3D spheroid culture methodology been developed for gastric cancer cells. The assessment cell spheroids' physical characteristics, such as size, morphology solidity, well inhibitors formation was...
In the scenario of personalized medicine, targeted therapies are currently focus cancer drug development. These drugs can block growth and spread tumor cells by interfering with key molecules involved in malignancy, such as receptor tyrosine kinases (RTKs). MET Recepteur d’Origine Nantais (RON), which RTKs frequently overactivated gastric cancer, glycoprotein receptors whose activation have been shown to be modulated cellular glycosylation. this work, we address role sialylation therapy...
Microsatellite instability (MSI) is a distinct molecular subtype of gastric cancer. In recent years, the clinical consequences MSI and therapeutic opportunities to target this peculiar cancer became evident. However, despite importance for stratification patients, time resources required diagnosis still present an obstacle. attempt identify new marker in cancer, we evaluated expression five cancer-associated glycan epitopes cohort 13 17 microsatellite stable (MSS) cases. Our analysis...
Abstract With the advent of personalized medicine, there is a movement to develop “smaller” and “smarter” microdevices that are able distinguish similar cancer subtypes. Tumor cells display major differences when compared their natural counterparts, due alterations in fundamental cellular processes such as glycosylation. Glycans involved tumor cell biology they have been considered be suitable biomarkers. Thus, more selective screening assays can developed through detection specific altered...
Here, we introduce a novel scFv antibody, G2-D11, specific for two adjacent Tn-antigens (GalNAc-Ser/Thr) binding equally to three dimeric forms of the epitope, Ser-Thr, Thr-Thr and Thr-Ser. Compared other anti-Tn reagents, G2-D11 is minimally influenced by peptide structure, which indicates high degree carbohydrate epitope dominance low influence from protein backbone. With affinity (KDapp = 1.3 × 10-8 M) no cross-reactivity either sialyl-Tn or blood group A antigens, an excellent candidate...
Gastric carcinoma MKN45 cells stably transfected with the full-length ST3GAL4 gene were characterised by glycomic and sialoproteomic analysis. Complementary strategies applied to assess alterations induced overexpression. The N- O-glycome data generated in two parallel structural analyzes, based on PGC-ESI-MS/MS. Data glycan structure identification relative abundance overexpressing respective mock control are presented. analysis titanium-dioxide enrichment of sialopeptides subsequent...
Abstract Triple negative breast cancer (TNBC) is a major global public health problem. The lack of targeted therapy and the elevated mortality evidence need for better knowledge tumor biology. Hypoxia aberrant glycosylation are associated with advanced stages malignancy, progression treatment resistance. Importantly, serum deprivation regulates invasive phenotype favors TNBC cell survival. However, in TNBC, role hypoxia regulation remains largely unknown. effects on expression...