A5030200432- Bladder and Urothelial Cancer Treatments
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Urinary and Genital Oncology Studies
- Epigenetics and DNA Methylation
- Proteoglycans and glycosaminoglycans research
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Colorectal Cancer Screening and Detection
- Hepatocellular Carcinoma Treatment and Prognosis
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- BIM and Construction Integration
- Drug Transport and Resistance Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Estrogen and related hormone effects
- Galectins and Cancer Biology
- Cancer-related molecular mechanisms research
- Cancer Mechanisms and Therapy
- Genomics and Phylogenetic Studies
Instituto Português de Oncologia Francisco Gentil
2015-2025
IPO Porto
2007-2025
Pontifícia Universidade Católica de Campinas
2024-2025
Universidade Estadual de Campinas (UNICAMP)
2025
Pontifícia Universidade Católica de São Paulo
2025
Universidade Federal de Jataí
2024
INESC TEC
2021-2024
Instituto do Câncer do Estado de São Paulo
2023
Universidade de São Paulo
2023
Research Network (United States)
2023
Little is known on the expression of tumour‐associated carbohydrate antigen sialyl‐Tn (STn), in bladder cancer. We report here that 75% high‐grade tumours, presenting elevated proliferation rates and high risk recurrence/progression expressed STn. However, it was mainly found non‐proliferative areas tumour, namely cells invading basal muscle layers. STn also tumour‐adjacent mucosa, which suggests its dependence a field effect tumour. Furthermore, not by normal urothelium, demonstrating...
Muscle invasive bladder cancer (MIBC, stage ≥T2) is generally associated with poor prognosis, constituting the second most common cause of death among genitourinary tumours. Due to high molecular heterogeneity significant variations in natural history and disease outcome have been observed. This has also delayed introduction personalized therapeutics, making advanced almost an orphan terms treatment. Altered protein glycosylation translated by expression sialyl-Tn antigen (STn) its precursor...
Invasive bladder tumours express the cell-surface Sialyl-Tn (STn) antigen, which stems from a premature stop in protein O-glycosylation. The STn antigen favours invasion, immune escape, and possibly chemotherapy resistance, making it attractive for target therapeutics. However, events leading to such deregulation glycosylation are mostly unknown. Since hypoxia is salient feature of advanced stage tumours, we searched into how influences cancer cells glycophenotype, with emphasis on...
Gastric cancer is a dominating cause of cancer-associated mortality with limited therapeutic options. Here, we show that syndecan-4 (SDC4), transmembrane proteoglycan, highly expressed in intestinal subtype gastric tumors and this signature associates patient poor survival. Further, mechanistically demonstrate SDC4 master regulator cell motility invasion. We also find decorated heparan sulfate efficiently sorted extracellular vesicles (EVs). Interestingly, EVs regulates cell-derived EV organ...
Bladder carcinogenesis and tumour progression is accompanied by profound alterations in protein glycosylation on the cell surface, which may be explored for improving disease management. In a search prognosis biomarkers novel therapeutic targets we have screened, using immunohistochemistry, series of bladder tumours with differing clinicopathology short‐chain O ‐glycans commonly found glycoproteins human solid tumours. These included Tn T antigens their sialylated counterparts sialyl‐Tn(STn)...
Rationale: Bladder cancer (BC) management demands the introduction of novel molecular targets for precision medicine.Cell surface glycoprotein CD44 has been widely studied as a potential biomarker BC aggressiveness and stem cells.However, significant alternative splicing multiple glycosylation generate myriad glycoproteoforms with potentially distinct functional roles.The lack tools precise characterization led to conflicting results, delaying clinical applications.Addressing these...
CD44 is a heavily glycosylated membrane receptor playing key role in cell adhesion, signal transduction and cytoskeleton remodelling. It also one of the most studied glycoproteins cancer, frequently explored for stem identification, associated with chemoresistance metastasis. However, general designation large family splicing variants exhibiting different degrees glycosylation and, potentially, functionally distinct roles. Moreover, structural diversity ambiguous nomenclature has delayed...
To evaluate the predictive value of genetic polymorphisms in context bacille Calmette-Guérin (BCG) immunotherapy outcome and create a profile that may allow discrimination risk recurrence.In dataset 204 patients treated with BCG, we evaluated 42 38 genes involved BCG mechanism action, using Sequenom MassARRAY(®) technology. Stepwise multivariate Cox regression was used for data mining.In agreement previous studies found gender, age, tumour multiplicity treatment scheme were associated...
Incomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as sialyl-Tn (STn) antigen. Despite all progress development potential anti-cancer antibodies, their application frequently hindered by low specificities and cross-reactivity. In this study, novel anti-STn monoclonal antibody named L2A5 was developed hybridoma technology. Flow cytometry analysis showed that specifically binds to sialylated structures on cell surface...
Muscle invasive bladder cancer (MIBC) remains amongst the deadliest genitourinary malignancies due to treatment failure and extensive molecular heterogeneity, delaying effective targeted therapeutics. Hypoxia nutrient deprivation, oversialylation O-glycans shortening are salient features of aggressive tumours, creating cell surface glycoproteome fingerprints with theranostics potential.A glycomics guided glycoproteomics workflow was employed identify potentially targetable biomarkers using...
This study aimed to investigate the influence of genetic variants in neuroplasticity-related genes on antidepressant treatment phenotypes. The BDNF-TrkB signaling pathway, as well downstream kinases Akt and ERK mTOR have been implicated depression neuroplasticity. However, clinicians still struggle with unpredictability responses depressed patients. We genotyped 26 polymorphisms BDNF, NTRK2, NGFR, CREB1, GSK3B, AKT, MAPK1, MTOR, PTEN, ARC, SYN1 80 patients major depressive disorder treated...
Early detection of colorectal cancer (CRC) significantly improves overall prognosis and increases 5-year survival rates up to 90%. Current non-invasive screening methods for CRC, such as the Faecal Immunohistochemical Test (FIT), have some drawbacks, namely, low sensitivity a high false-positive rate. The Sialyl-Tn (STn) antigen, frequently expressed in pre-malignant lesions adenocarcinomas, has been shown be detected by novel monoclonal antibody L2A5. In this study, we explored potential...
O presente trabalho analisa a responsabilidade civil dos influenciadores digitais na promoção das casas de apostas, com foco proteção jurídica menores idade. Diante do crescimento exponencial dessas plataformas e impacto da publicidade digital, discute-se como os influenciadores, ao promoverem esses serviços, podem induzir jovens jogo azar, tornando-se potencialmente responsáveis por danos causados. A pesquisa adota uma abordagem exploratória explicativa, utilizando análise qualitativa...