A5066687709- Biosensors and Analytical Detection
- Listeria monocytogenes in Food Safety
- Identification and Quantification in Food
- Salmonella and Campylobacter epidemiology
- Advanced biosensing and bioanalysis techniques
- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Nanocomposite Films for Food Packaging
- Advanced Chemical Sensor Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Antimicrobial agents and applications
- Nanoparticles: synthesis and applications
- Gold and Silver Nanoparticles Synthesis and Applications
- SARS-CoV-2 detection and testing
- Advanced Biosensing Techniques and Applications
- Mycotoxins in Agriculture and Food
- Essential Oils and Antimicrobial Activity
- Environmental Toxicology and Ecotoxicology
- Plant Pathogens and Fungal Diseases
- Environmental DNA in Biodiversity Studies
- Antibiotic Resistance in Bacteria
Instituto de Investigacións Mariñas
2024-2025
Consejo Superior de Investigaciones Científicas
2025
International Iberian Nanotechnology Laboratory
2017-2024
University of Teramo
2024
Universidade de Santiago de Compostela
2024
Universidad Autónoma de Madrid
2019
Clinical Academic Center of Braga
2019
Instituto de Investigaciones Biomédicas Sols-Morreale
2019
University of Florida
2017-2018
ANFACO-CECOPESCA
2010-2015
Herein, we demonstrated the potential of surface-enhanced Raman scattering (SERS) spectroscopy combined with microfluidics for detection and discrimination foodborne pathogens. SERS-tagged gold nanostars were functionalized a monoclonal antibody specific Listeria monocytogenes. In presence L. monocytogenes, SERS signal corresponding to tag paired was detected in real time continuous flow, enabling monocytogenes innocua just 100 s. To best our knowledge, this is first that tags have been used...
Microbiological analysis of food is an integrated part microbial safety management in the chain. Monitoring and controlling foodborne pathogens are traditionally carried out by conventional microbiological methods based on culture-dependent approaches control laboratories private companies. However, polymerase chain reaction (PCR) has revolutionized allowing detection pathogenic microorganisms food, without necessity classical isolation identification. at present, PCR quantitative (qPCR)...
Foodborne diseases are an important cause of morbidity and mortality. According to the World Health Organization, there 31 main global hazards, which caused in 2010 600 million foodborne illnesses 420000 deaths. Among them, Salmonella spp. is one most human pathogens, accounting for more than 90000 cases Europe even United States per year. In current study we report development, thorough evaluation food samples, a microfluidic system combining loop-mediated isothermal amplification with gold...
An amplification-free SERS chip for cellular DNA mutation analysis has been developed.
We developed a droplet-based optofluidic system for the detection of foodborne pathogens. Specifically, loop-mediated isothermal amplification (LAMP) technique was combined with surface-enhanced Raman scattering (SERS), which offers an excellent method DNA ultradetection. However, direct SERS compromises simplicity data interpretation due to variability its fingerprints. Therefore, we designed indirect using multifunctional gold nanoparticles (AuNPs) based on formation pyrophosphate...
Ready-to-eat products, such as leafy greens, must be carefully controlled they are directly consumed without any treatment to reduce the presence of potential pathogens. Food industries, especially those that process products with short shelf-life, demand rapid detection foodborne pathogens Shiga Toxin-producing Escherichia coli (STEC). In this sense, molecular methods can fulfill both requirements turnaround time and consumer safety. The most popular based on real-time PCR (qPCR) however,...
Salmonella Enteritidis is the main serotype responsible for human salmonellosis in European Union. One of sources spp. food chain are poultry products, such as eggs or chicken meat. In recent years, molecular methods have become an alternative to culture dependent rapid screening this work, strain S. S1400, and previously isolated characterized bacteriophage PVP-SE2, were used develop evaluate a same-day detection method combining Phage Amplification Loop-mediated isothermal amplification...
Loop-mediated isothermal amplification, LAMP, is nowadays the most popular nucleic acid amplification technique, and as such, several commercial, ready-to-use master mixes have flourished. Unfortunately, independent studies to determine their performance are limited. The current study performed an evaluation of existing commercial LAMP WarmStart® Kit, LavaLAMP™ DNA Master Mix, Saphir Bst Turbo GreenMaster, OptiGene Fast Mix ISO-004, SynLAMP Mix. To reduce bias, three different genes, namely...
Klebsiella pneumoniae is an opportunistic human pathogen of high relevance due to its ability acquire antibiotic resistance. This included, along with Enterococcus faecium, Staphylococcus aureus, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter spp., in the ESKAPE group, which consists most important bacterial pathogens resistant antibiotics clinical setups. Due importance rapid identification infection-causative agents, a novel method for K. was developed present work. based...
Abstract Nowadays, Listeria monocytogenes continues to be a major health issue. Therefore, improvements in the speed and reliability of its detection are still needed. In present study, combination real‐time Recombinase Polymerase Amplification (qRPA) with immunomagnetic separation (IMS) is described. The proposed methodology was tested against PCR method, successfully applied 50 smoked salmon samples spiked at levels ranging from 2 9.3 × 10 cfu/25 g. L. detected after 24 hr pre‐enrichment,...