A5045584014- Glycosylation and Glycoproteins Research
- Chronic Lymphocytic Leukemia Research
- Rheumatoid Arthritis Research and Therapies
- Acute Lymphoblastic Leukemia research
- Cancer Research and Treatments
- Bladder and Urothelial Cancer Treatments
- Advanced Proteomics Techniques and Applications
- RNA modifications and cancer
- Autoimmune and Inflammatory Disorders Research
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Alzheimer's disease research and treatments
- Proteoglycans and glycosaminoglycans research
- Genomics and Phylogenetic Studies
- Urinary and Genital Oncology Studies
- Cell Adhesion Molecules Research
- Galectins and Cancer Biology
- Immunotherapy and Immune Responses
- Mobile Health and mHealth Applications
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Bacterial Identification and Susceptibility Testing
- Carbohydrate Chemistry and Synthesis
- Biological Research and Disease Studies
- Genetic Associations and Epidemiology
Institut Pasteur
2024-2025
Centre National de la Recherche Scientifique
2025
Universidad Complutense de Madrid
2025
IPO Porto
2001-2024
University of Turku
2021-2024
University of Trás-os-Montes and Alto Douro
2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022-2024
Technologies pour la Santé
2022-2024
Université Paris-Saclay
2022-2024
Invasive bladder tumours express the cell-surface Sialyl-Tn (STn) antigen, which stems from a premature stop in protein O-glycosylation. The STn antigen favours invasion, immune escape, and possibly chemotherapy resistance, making it attractive for target therapeutics. However, events leading to such deregulation glycosylation are mostly unknown. Since hypoxia is salient feature of advanced stage tumours, we searched into how influences cancer cells glycophenotype, with emphasis on...
Methotrexate (MTX) is used for rheumatoid arthritis (RA) treatment showing a wide toxicity profile. This study aimed to evaluate the influence of single nucleotide polymorphisms (SNPs) in genes encoding MTX transporters with occurrence MTX-related (overall and gastrointestinal). A total 233 Portuguese RA patients were genotyped 23 SNPs. Haplotype analyses performed toxicogenetic risk index (TRI) was created SNPs that revealed be statistically significant. Regarding overall toxicity, an...
Bladder carcinogenesis and tumour progression is accompanied by profound alterations in protein glycosylation on the cell surface, which may be explored for improving disease management. In a search prognosis biomarkers novel therapeutic targets we have screened, using immunohistochemistry, series of bladder tumours with differing clinicopathology short‐chain O ‐glycans commonly found glycoproteins human solid tumours. These included Tn T antigens their sialylated counterparts sialyl‐Tn(STn)...
Rationale: Bladder cancer (BC) management demands the introduction of novel molecular targets for precision medicine.Cell surface glycoprotein CD44 has been widely studied as a potential biomarker BC aggressiveness and stem cells.However, significant alternative splicing multiple glycosylation generate myriad glycoproteoforms with potentially distinct functional roles.The lack tools precise characterization led to conflicting results, delaying clinical applications.Addressing these...
CD44 is a heavily glycosylated membrane receptor playing key role in cell adhesion, signal transduction and cytoskeleton remodelling. It also one of the most studied glycoproteins cancer, frequently explored for stem identification, associated with chemoresistance metastasis. However, general designation large family splicing variants exhibiting different degrees glycosylation and, potentially, functionally distinct roles. Moreover, structural diversity ambiguous nomenclature has delayed...
Muscle invasive bladder cancer (MIBC) remains amongst the deadliest genitourinary malignancies due to treatment failure and extensive molecular heterogeneity, delaying effective targeted therapeutics. Hypoxia nutrient deprivation, oversialylation O-glycans shortening are salient features of aggressive tumours, creating cell surface glycoproteome fingerprints with theranostics potential.A glycomics guided glycoproteomics workflow was employed identify potentially targetable biomarkers using...
ABSTRACT Aim This prospective cohort study aimed to evaluate the incidence and risk/protective factors of peri‐implantitis over time. Methods A university‐representative was evaluated at baseline after a mean follow‐up time 3.9 years. The main outcome peri‐implantitis, defined as bone loss > 1 mm between two examinations in implants showing bleeding on probing. Putative assessed were tested through multilevel (mixed‐effects) logistic regression analyses. Results total 73 patients with 322...
The protozoan parasite Trypanosoma brucei is a mono-flagellated cell during the G1-phase of its cycle. In order to duplicate, it assembles new flagellum alongside mature one, in which further elongation prevented. Our group proposed model where locked after construction full length (Bertiaux et al. 2018) and access building blocks for exclusive flagellum. To test this hypothesis directly, we developed tool inducible expression tagged tubulin. Alpha-tubulin that was with an intragenic...
Background Therapeutic outcome of rheumatoid arthritis (RA) patients treated with methotrexate (MTX) can be modulated by thymidylate synthase (TS) levels, which may altered genetic polymorphisms in TS gene (TYMS). This study aims to elucidate the influence TYMS MTX therapeutic (regarding both clinical response and toxicity) Portuguese RA patients. Methods Clinicopathological data from 233 Caucasian were collected, outcomes defined genotyped for following polymorphisms: 1) 28 base pairs (bp)...
Methotrexate (MTX) is widely used for rheumatoid arthritis (RA) treatment. Single nucleotide polymorphisms (SNPs) could be as predictors of patients' therapeutic outcome variability. Therefore, this study aims to evaluate the influence SNPs in genes encoding MTX membrane transport proteins order predict clinical response MTX. Clinicopathological data from 233 RA patients treated with were collected, defined, and genotyped 23 SNPs. Genotype haplotype analyses performed using multivariate...
Objective. Methotrexate (MTX), the most used drug in rheumatoid arthritis (RA) treatment, showing variability clinical response, is often associated with genetic polymorphisms. This study aimed to elucidate role of methylenetetrahydrofolate reductase ( MTHFR ) C677T and aminoimidazole carboxamide adenosine ribonucleotide transformylase ATIC T675C polymorphisms clinicopathological variables response MTX Portuguese RA patients. Methods. Study included 233 patients treated for at least six...
Aim: Evaluate the potential of selected SNPs as predictors methotrexate (MTX) therapeutic outcome. Patients & methods: In total, 35 in 14 genes involved MTX intracellular pathways and Phase II reactions were genotyped 233 rheumatoid arthritis (RA) patients treated with MTX. Binary logistic regressions performed by genotype/haplotype-based approaches. Non-Response- Toxicity-Genetic Risk Indexes (Non-RespGRI ToxGRI) created. Results: nonresponse was associated to eight genotypes three...
Background: Gastric cancer (GC) is a major health burden worldwide, with half of patients developing metastases within 5 years after treatment, urging novel biomarkers for diagnosis and efficient therapeutic targeting. Sialyl-Lewis A (SLeA), terminal glycoepitope glycoproteins glycolipids, offers tremendous potential towards this objective. It rarely expressed in healthy tissues blood cells, while it present highly metastatic cell lines metastases. SLeA also involved E-selectin mediated...
Aim: The aim of our study was to characterize the association clinicopathological variables and SLC19A1/RFC-1 G80A polymorphism in methotrexate (MTX)-related toxicity Portuguese patients with rheumatoid arthritis. Patients & methods: included 233 consecutively recruited arthritis under MTX treatment. SLC19A1 evaluated by PCR-RFLP. Results: Statistical analysis revealed that 80G carriers had increased risk gastrointestinal (odds ratio [OR]: 2.61, p = 0.019) regular folic acid supplementation...