A5066319573- Salivary Gland Disorders and Functions
- Advanced Proteomics Techniques and Applications
- Oral microbiology and periodontitis research
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- Antimicrobial Peptides and Activities
- Muscle Physiology and Disorders
- Muscle metabolism and nutrition
- Glycosylation and Glycoproteins Research
- Adipose Tissue and Metabolism
- Biochemical Analysis and Sensing Techniques
- Metabolomics and Mass Spectrometry Studies
- Exercise and Physiological Responses
- Bladder and Urothelial Cancer Treatments
- Peptidase Inhibition and Analysis
- Biochemical effects in animals
- Cardiovascular Effects of Exercise
- Extracellular vesicles in disease
- Estrogen and related hormone effects
- Antioxidant Activity and Oxidative Stress
- Electron Spin Resonance Studies
- Lignin and Wood Chemistry
- Cancer Research and Treatments
- Nutrition and Health in Aging
- Advanced Glycation End Products research
University of Aveiro
2015-2025
Rede de Química e Tecnologia
2004-2025
Meharry Medical College
2022
University of Minho
2016
Escola Superior de Saúde do Alcoitão
2013-2014
RMIT University
2013-2014
Celulose Beira Industrial (Portugal)
2014
Deleted Institution
2014
Selangor Business School
2014
University of Évora
2012
Abstract The determination of salivary biomarkers as a means monitoring general health and for the early diagnosis disease is increasing interest in clinical research. Based on linkage between proteins systemic diseases, aim this work was identification saliva using proteomics. Salivary were separated two‐dimensional (2‐D) gel electrophoresis over pH range 3–10, digested, then analyzed by matrix assisted laser desorption/ionization‐time flight (MALDI‐TOF)‐TOF mass spectrometry (MS) tandem...
Results of a comprehensive study on the chemical structure lignin from plantation Eucalyptus globulus Labill are presented. Lignin has been isolated by modified mild acidolysis method and thoroughly characterized functional group analysis, series degradation techniques (nitrobenzene oxidation, permanganate thioacidolysis, Py-GC-MS), (1)H (13)C NMR spectroscopy. Plantation was found to be S/G type with an extremely high proportion syringyl (S) units (82-86%) minor p-hydrophenyl propane (H)...
Tannins are well-known food polyphenols that interact with proteins, namely, salivary proteins. This interaction is an important factor in relation to their bioavailability and considered the basis of several properties tannins, development astringency. It has been generally accepted astringency due tannin-induced complexation and/or precipitation proline-rich proteins (PRPs) oral cavity. However, this thought provide protection against dietary tannins. Neverthless, there no concrete...
Skeletal muscle is a highly specialized tissue that contains two distinct mitochondria subpopulations, the subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria. Although it established these mitochondrial subpopulations differ functionally in several ways, limited information exists about proteomic differences underlying functional differences. Therefore, objective of this study was to biochemically characterize SS IMF isolated from rat red gastrocnemius skeletal muscle. We separated...
In the present study, a proteomic approach was applied to evaluate influence of salivary protein composition on in vitro dental pellicle formation and its possible correlation with caries. Whole saliva, collected from caries‐free caries‐susceptible subjects, analyzed by two‐dimensional electrophoresis, spots were identified mass spectrometry. Data analysis showed statistically significant between quantity acidic proline‐rich proteins (PRPs), lipocalin, cystatin SN S, samples group subjects...
Many studies have investigated the protective effects of oleuropein and hydroxytyrosol against cell injury, but few aglycones 3,4-dihydroxyphenylethanol-elenolic acid (3,4-DHPEA-EA) dialdehyde (3,4-DHPEA-EDA). The present work studied compared capacity these four compounds, found at high concentrations in olive oil, to protect red blood cells (RBCs) from oxidative injury. vitro stress RBCs was induced by water-soluble radical initiator 2,2'-azo-bis(2-amidinopropane) dihydrochloride. RBC...
Little is known on the expression of tumour‐associated carbohydrate antigen sialyl‐Tn (STn), in bladder cancer. We report here that 75% high‐grade tumours, presenting elevated proliferation rates and high risk recurrence/progression expressed STn. However, it was mainly found non‐proliferative areas tumour, namely cells invading basal muscle layers. STn also tumour‐adjacent mucosa, which suggests its dependence a field effect tumour. Furthermore, not by normal urothelium, demonstrating...
Biomedical sciences, and in particular biomarker research, demand efficient glycoprotein enrichment platforms. Herein magnetic nanoprobes (MNP), after being coated with three broad-spectrum lectins-concanavalin A (ConA), wheat germ agglutinin (WGA), Maackia amurensis lectin (MA)-were utilized to selectively capture glycoproteins from human body fluids. Additionally, a new methodology, based on protection of the lectins their target sugars prior coupling MNPs, was proposed overcome...
Progression to hormone‐independent growth leading endocrine therapy resistance occurs in a high proportion of patients with estrogen receptor alpha (ERα) and progesterone receptors (PR) positive breast cancer. We others have previously shown that estrogen‐ progestin‐induced tumor requires ERα PR interaction at their target genes. Here, we show fibroblast factor 2 (FGF2)‐induces cell proliferation through activation the MYC enhancer proximal promoter. inhibitors, antiestrogens or...
Exosomes are small extracellular vesicles (EVs) present in human biofluids that can transport specific disease-associated molecules. Consequently blood-derived exosomes have emerged as important peripheral biomarker sources for a wide range of diseases, among them Alzheimer's disease (AD). Although there is no effective cure AD, an accurate diagnosis, relying on easily accessible biofluids, still necessary to discriminate this from other dementias, test potential therapies and even monitor...
Dental caries is a complex disease, characterized by demineralization of tooth structure. With protective role, several salivary phosphopeptides appear to be involved in remineralization processes, delaying the loss In this work we have correlated peptide saliva composition with dental susceptibility through analysis and hydroxyapatite-adsorbed peptides samples. Saliva samples were obtained from two groups, caries-free cariessusceptible group, analysed using HPLC-MS sequential extraction 6 m...
Abstract The major components of industrial thin (SSL) and thick (THSL) liquors from acidic magnesium–based sulphite pulping Eucalyptus globulus wood have been characterized. analysis sugars in SSL revealed the predominance xylose, which was present form sugar monomer (ca. 70%) as xylo-oligosaccharides 30%). Lignosulphonates (LS) were most abundant organic fraction spent liquor 50% dry matter) consisting sulphonated oligomers (SO3H ≈ 20% w/w) low molecular weight (Mw 1000–1300 Da)...
Gastric cancer is preceded by a carcinogenesis pathway that includes gastritis caused Helicobacter pylori infection, chronic atrophic may progress to intestinal metaplasia (IM), dysplasia, and ultimately gastric carcinoma of the more common subtype. The identification glycosylation changes in circulating serum proteins patients with precursor lesions high interest represents source putative new biomarkers for early diagnosis intervention. This study applies glycoproteomic approach identify...
In the present study, we applied iTRAQ-based quantitative approach to explore salivary proteome and peptidome profile in selected subjects with type 1 diabetes, without microvascular complications, aiming identify disease-related markers. From a total of 434 distinct proteins, bactericidal/permeability-increasing protein-like pancreatic adenocarcinoma up-regulated factor were found higher levels saliva all patients while increased content other proteins like alpha-2-macroglobulin, defensin...
Despite the importance of saliva in regulation oral cavity homeostasis, few studies have been conducted to quantitatively compare different mammal species. Aiming define a proteome signature mammals' saliva, an in-depth SDS-PAGE–LC coupled MS/MS (GeLC–MS/MS) approach was used characterize from primates (human), carnivores (dog), glires (rat and rabbit), ungulates (sheep, cattle, horse). high variability number distinct proteins identified per species, most protein families were shared by...