A5102821567- Alzheimer's disease research and treatments
- Extracellular vesicles in disease
- Cholinesterase and Neurodegenerative Diseases
- Spectroscopy Techniques in Biomedical and Chemical Research
- MicroRNA in disease regulation
- Bioinformatics and Genomic Networks
- Metabolomics and Mass Spectrometry Studies
- Computational Drug Discovery Methods
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Spectroscopy and Chemometric Analyses
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Dementia and Cognitive Impairment Research
- Prion Diseases and Protein Misfolding
- Therapeutic Uses of Natural Elements
- Cardiovascular Disease and Adiposity
- Genomics and Chromatin Dynamics
- S100 Proteins and Annexins
- Psychology and Mental Health
- Medicinal Plants and Neuroprotection
- 14-3-3 protein interactions
- Endoplasmic Reticulum Stress and Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Geriatric Care and Nursing Homes
- Palliative Care and End-of-Life Issues
University of Aveiro
2016-2025
Universidade do Porto
2022
The potential of exosomes as biomarker resources for diagnostics, prognostics and even therapeutics is an area intense research. Despite the various approaches available, there no consensus with respect to best methodology isolating provide substantial yields reliable quality. Differential centrifugation most commonly used method but it time-consuming requires large sample volumes, thus alternative methods are urgently needed. In this study two precipitation-based one column-based approach...
Alzheimer's disease is a challenge in modern healthcare due to its complex etiology and increasing prevalence. Despite advances, further understanding of pathophysiology needed, particularly the role Aβ neurotoxic peptide. Fourier transform infrared spectroscopy (FTIR) has shown potential as screening tool for several pathologies, including disease. Nonetheless, limited research explored direct effects on neurons extracellular vesicles metabolic profiles. Hence, this study aims investigate...
Exosomes are small extracellular vesicles (EVs) present in human biofluids that can transport specific disease-associated molecules. Consequently blood-derived exosomes have emerged as important peripheral biomarker sources for a wide range of diseases, among them Alzheimer's disease (AD). Although there is no effective cure AD, an accurate diagnosis, relying on easily accessible biofluids, still necessary to discriminate this from other dementias, test potential therapies and even monitor...
Alzheimer's disease (AD) diagnosis is based on psychological and imaging tests but can also include monitoring cerebrospinal fluid (CSF) biomarkers. However, CSF based-neurochemical approaches are expensive invasive, limiting their use to well-equipped settings. In contrast, blood-based biomark ers minimally cost-effective, a widely accessible alternative. Blood-derived exosomes have recently emerged as reliable AD biomarker source, carrying disease-specific cargo. Fourier-transformed...
J. Neurochem. (2010) 113 , 761–771. Abstract Aβ is proteolytically produced from the Alzheimer’s amyloid precursor protein (APP). Major properties attributed to include neurotoxic effects that contribute disease neurodegeneration. However, can also affect APP processing and trafficking that, in neurons, anterogradelly transported via microtubules a kinesin‐associated manner. Herein we show induce accumulation of intracellular sAPP primary neuronal cultures. Subcellular fractionation studies...
Two histopathological hallmarks of Alzheimer's disease (AD), the tau rich neurofibrillary tangles and senile plaques, predominating in amyloid-β (Aβ), have fueled research distinct directions. Evidence suggests that Aβ triggers imbalanced acti
Abstract The amyloidogenic peptide, Aβ, provokes a series of events affecting distinct cellular pathways regulated by protein phosphorylation. Aβ inhibits phosphatases in dose-dependent manner, thus it is expected that the phosphorylation state specific proteins would be altered response to Aβ. In fact several Alzheimer’s disease related proteins, such as APP and TAU, exhibit pathology associated hyperphosphorylated states. A systems biology approach was adopted phosphoproteome, primary...
Abstract Protein aggregation is remarkably associated with several neuropathologies, including Alzheimer´s (AD) and Parkinson´s disease (PD). The first characterized by hyperphosphorylated tau protein Aβ peptide deposition, thus forming intracellular neurofibrillary tangles extracellular senile plaques, respectively; while, in PD, α-synuclein aggregates deposits as Lewy bodies. Considerable research has focused on developing models to be explored tools. In the present work, four vitro for...
The potential of exosomes as biomarker resources for diagnostics and even therapeutics has intensified research in the field, including context Alzheimer´s disease (AD). search biomarkers peripheral biofluids is advancing mainly due to easy access it offers. In study presented here, emphasis was given bioinformatic identification putative exosomal candidates AD. proteomes cerebrospinal fluid (CSF), serum plasma, were obtained from three databases (ExoCarta, EVpedia Vesiclepedia),...
Proteolytic processing of the amyloid-β protein precursor (AβPP) occurs via alternative pathways, culminating with production AβPP intracellular domain (AICD). AICD can translocate to nucleus and regulate transcription, but its activit
<b><i>Background/Aims:</i></b> Diagnosing dementia is challenging in many primary care settings, given the limited human resources and lack of current diagnostic tools. With this mind, a care-based cohort was established Aveiro district Portugal. <b><i>Methods:</i></b> A total 568 participants were evaluated using cognitive tests APOE genotyping. <b><i>Results:</i></b> The findings revealed prevalence 12%. strong...
Phosphorylation plays a key role in Alzheimer's disease (AD) pathogenesis, impacting distinct processes such as amyloid-beta (Aβ) peptide production and tau phosphorylation. Impaired phosphorylation events contribute to senile plaques neurofibrillary tangles' formation, two major histopathological hallmarks of AD. Blood-derived extracellular particles (bdEP) can represent disease-related source phosphobiomarker candidates, hence, this pilot study, bdEP Control AD cases were analyzed by...
Alzheimer's disease (AD) diagnosis is difficult, and new accurate tools based on peripheral biofluids are urgently needed. Extracellular vesicles (EVs) emerged as a valuable source of biomarker profiles for AD, since their cargo disease-specific these can be easily isolated from accessible biofluids, blood. Fourier Transform Infrared (FTIR) spectroscopy employed to analyze EVs obtain the spectroscopic different regions spectra, simultaneously characterizing carbohydrates, nucleic acids,...