A5006723975- Salivary Gland Disorders and Functions
- Oral microbiology and periodontitis research
- dental development and anomalies
- Cleft Lip and Palate Research
- Craniofacial Disorders and Treatments
- Advanced Proteomics Techniques and Applications
- Dental materials and restorations
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Dental Health and Care Utilization
- Dental Implant Techniques and Outcomes
- Bone and Dental Protein Studies
- Connexins and lens biology
- Dental Research and COVID-19
- Anatomy and Medical Technology
- Protein Hydrolysis and Bioactive Peptides
- Digital Imaging in Medicine
- Oral and Maxillofacial Pathology
- Dental Erosion and Treatment
- Fluoride Effects and Removal
- Oral and gingival health research
- Machine Learning in Bioinformatics
- Extracellular vesicles in disease
- Endodontics and Root Canal Treatments
Cooperativa de Ensino Superior Politécnico e Universitário
2006-2023
Abstract The determination of salivary biomarkers as a means monitoring general health and for the early diagnosis disease is increasing interest in clinical research. Based on linkage between proteins systemic diseases, aim this work was identification saliva using proteomics. Salivary were separated two‐dimensional (2‐D) gel electrophoresis over pH range 3–10, digested, then analyzed by matrix assisted laser desorption/ionization‐time flight (MALDI‐TOF)‐TOF mass spectrometry (MS) tandem...
In the present study, a proteomic approach was applied to evaluate influence of salivary protein composition on in vitro dental pellicle formation and its possible correlation with caries. Whole saliva, collected from caries‐free caries‐susceptible subjects, analyzed by two‐dimensional electrophoresis, spots were identified mass spectrometry. Data analysis showed statistically significant between quantity acidic proline‐rich proteins (PRPs), lipocalin, cystatin SN S, samples group subjects...
Abstract Human acquired enamel pellicle is the result of a selective interaction salivary proteins and peptides with tooth surface. In present work, characterization as well type interactions established surface was performed. Peptides from in vivo bovine implants human oral cavity were sequentially extracted using guanidine trifluoroacetic acid solutions fractions obtained analysed by LC‐MS LC‐MS/MS. Based on data, six phosphorylated identified an intact form, strongly adsorbed to Data...
Abstract Proline‐rich proteins (PRPs) are the most complex family of salivary peptides with distinct isoforms and PTMs. Up to date, only serine phosphorylation at positions 8, 17, 22 have been experimentally observed on acidic PRP (aPRPs), position 8 basic PRP1 2. The presence a glucoronyl group Ser17 was also noticed aPRP. main goal this study identify new PTMs PRPs using LC‐MALDI‐TOF/TOF. Through peptidome characterization 20 different subjects from Control, Diabetic, Head Neck Cancer...
Abstract The present study proposes a strategy for human in vivo acquired enamel pellicle (AEP) peptidome characterisation based on sequential extraction with guanidine and TFA followed by MALDI‐TOF/TOF identification. Three different nanoscale analytical approaches were used: samples subjected to tryptic digestion nano‐HPLC mass spectrometry (MS MS/MS) analysis. Undigested analysed LC‐MS (both linear reflector modes) LC‐MS/MS analysis, on‐plate majority of the identifications corresponded...
ABSTRACT Diabetic patients show a high susceptibility to oral diseases of inflammatory, catabolic and chronic nature with potential impact on saliva composition. In this study, our purpose was characterize type 1 diabetes‐induced alterations in the salivary peptidome aiming find prospective biomarkers for diabetes health evaluation. Peptidomic analysis from controls ( n = 5) diabetic were performed by liquid chromatography followed mass spectrometry. The proteolytic activity...
Salivary peptides are involved in a wide range of functions constituting the first line defence oral cavity and precursors dental pellicle formation. The presence mucins saliva makes difficult analysis proteic content. This is due mainly to aggregation phenomenon between other high molecular weight glycoproteins salivary proteins. Considering importance biological functions, we have evaluated influence four different extraction methodologies on separation identification these proteins by...
Background.Mandibular second premolar (M2P) agenesis results in the primary molar (2pm) retention, infraocclusion, a reduced alveolar height and width, supraeruption of antagonists, or movement adjacent teeth.Infraocclusion affects survival retained 2pm to greater extent than root resorption.Objectives.The aim study was evaluate lifespan as substitute, with quality occlusal adaptation, cases M2P low-income population determine if attitude just vigilance could be best clinical option whenever...
This article was originally published in Proteomics 2010, 10 , 3732–3742, DOI 10.1002/pmic.200900261
Traditional load-to-failure tests fail to recreate clinical failures of all-ceramic restorations. Experimental fabrication, similar prosthetic laboratory and procedures, best predicts future performance. A hybrid ceramic adherend, mechanically a human tooth, was tested by comparing the shear bond strength (SBS) fracture mode four restorative materials adhered with dual-cure adhesive cement. Surface energy, (SBS), were assessed. Vita Enamic (ENA), Suprinity (SUP), Y-TPZ (Y-ZT), nanohybrid...
Maxillary lateral incisor agenesis (MLIA), treated orthodontically by space opening, requires complimentary aesthetic rehabilitation. Resin-bonded bridges (RBBs) can be equated as interim rehabilitation until skeletal maturity is achieved to place an implant-supported crown or definitive in case of financial restrictions implant contraindications. Scientific evidence the best material must confirmed specific clinical situations. Computer-aided design and computer-aided manufacturing...