A5088906026- Advanced Proteomics Techniques and Applications
- Oral microbiology and periodontitis research
- Cardiovascular Function and Risk Factors
- Cardiac Valve Diseases and Treatments
- Salivary Gland Disorders and Functions
- Mass Spectrometry Techniques and Applications
- Protease and Inhibitor Mechanisms
- Antimicrobial Peptides and Activities
- Metabolomics and Mass Spectrometry Studies
- Cardiac Fibrosis and Remodeling
- Cardiovascular Issues in Pregnancy
- Cardiovascular Effects of Exercise
- Oral and gingival health research
- Bioinformatics and Genomic Networks
- Cardiomyopathy and Myosin Studies
- Peptidase Inhibition and Analysis
- Aortic aneurysm repair treatments
- Infective Endocarditis Diagnosis and Management
- Biotin and Related Studies
- Machine Learning in Bioinformatics
- Cardiac pacing and defibrillation studies
- Oral Health Pathology and Treatment
- Atrial Fibrillation Management and Outcomes
- Cardiovascular Disease and Adiposity
- Heart Failure Treatment and Management
Universidade do Porto
2016-2025
University of Aveiro
2014-2021
Universitat Autònoma de Barcelona
2013
Despite the importance of saliva in regulation oral cavity homeostasis, few studies have been conducted to quantitatively compare different mammal species. Aiming define a proteome signature mammals' saliva, an in-depth SDS-PAGE–LC coupled MS/MS (GeLC–MS/MS) approach was used characterize from primates (human), carnivores (dog), glires (rat and rabbit), ungulates (sheep, cattle, horse). high variability number distinct proteins identified per species, most protein families were shared by...
Vascular calcification (VC) is a frequent condition in chronic kidney disease (CKD) and well-established risk factor for the development of cardiovascular (CVD). Gut dysbiosis may contribute to CVD inflammation CKD patients. Nonetheless, role gut blood microbiomes CKD-associated VC remains unknown. Therefore, this pilot study aimed explore link between patients on peritoneal dialysis (CKD-PD). Our results showed relative changes specific taxa CKD-PD with without VC, namely Coprobacter,...
Deregulation of tRNAs, aminoacyl-tRNA synthetases and tRNA modifying enzymes are common in cancer, raising the hypothesis that protein synthesis efficiency accuracy (mistranslation) compromised tumors. We show here human colon tumors xenograft produced mice by two epithelial cancer cell lines mistranslate 2- to 4-fold more frequently than normal tissue. To clarify if mistranslation plays a role tumor biology, we expressed mutant Ser-tRNAs misincorporate Ser-at-Ala (frequent error) Ser-at-Leu...
Several studies have demonstrated that administration of doxorubicin (DOXO) results in cardiotoxicity, which eventually progresses to dilated cardiomyopathy. The present work aimed evaluate the early myocardial changes DOXO-induced cardiotoxicity. Male New Zealand White rabbits were injected intravenously with DOXO twice weekly for 8 wk [DOXO-induced heart failure (DOXO-HF)] or an equivolumetric dose saline (control). Echocardiographic evaluation was performed, and samples collected cellular...
Chronic periodontitis (CP) is a complex immuno-inflammatory disease that results from preestablished gingivitis. We investigated potential differences in salivary peptidome health and CP.Saliva was collected nine CP patients ten healthy subjects, which five were enriched following endoProteoFASP approach, separated identified by nanoHPLC-MALDI-TOF/TOF. Protease prediction carried out silico with Proteasix. Parallel gelatin collagen (I) zymographies performed to study proteolytic activity...
Heart failure (HF) is among the leading causes of morbidity and mortality worldwide. Several conditions trigger left ventricular chronic pressure or volume overload, hypertrophy, systolic diastolic dysfunction, to cardiac remodeling a rapid progression toward HF. Therapeutic interventions elicit reverse (RR), highly variable myocardial response that ranges from none total structural/functional recovery. However, HF patients present several comorbidities medications mask comprehensive...
Heart failure with preserved ejection fraction (HFpEF) is a syndrome characterized by impaired cardiovascular reserve in which therapeutic options are scarce. Our aim was to evaluate the inodilator levosimendan ZSF1 obese rat model of HFpEF. Twenty-week-old male Wistar-Kyoto (WKY), lean (ZSF1 Ln) and rats chronically treated for 6-weeks either (1 mg/kg/day, Ob + Levo) or vehicle Veh) underwent peak-effort testing, pressure-volume (PV) haemodynamic evaluation echocardiography (n = 7 each)....
To identify new protein targets for PCa detection, first, a shotgun discovery experiment was performed to characterize the urinary proteome of patients. This revealed 18 differentially abundant proteins in Second, selected were clinically tested by immunoblot, and soluble E-cadherin fragment detected first time urine Third, proteogenome landscape these patients characterized, revealing 1665 mutant isoforms. Statistical analysis 6 isoforms Analysis likely effects mutations on function PPIs...
This study describes the impact of cardiovascular risk factors (CVR) in pregnancy-induced remodeling and postpartum reverse (up to 1 yr) by applying advanced statistic methods (multivariate generalized linear mixed-effects models) a prospective cohort pregnant women. Aiming extrapolate pathological conditions, this invaluable “human model” allowed us demonstrate that arterial hypertension is critical CVR for worse RR ST2/IL33-receptors CRP are potential biomarkers hypertrophy reversal.
Coronary artery disease (CAD) and the frequently coexisting aortic valve stenosis (AVS) are heart diseases accounting for most cardiac surgeries. These share many risk factors, such as age, diabetes, hypertension, or obesity, similar pathogenesis, including endothelial disruption, lipid immune cell infiltration, inflammation, fibrosis, calcification. Unsuspected CAD AVS sometimes detected opportunistically through echocardiography, coronary angiography, magnetic resonance. Routine biomarkers...