A5065164000- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- AI in cancer detection
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Cervical Cancer and HPV Research
- Pain Mechanisms and Treatments
- Immune Cell Function and Interaction
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Machine Learning in Bioinformatics
- Genetics, Aging, and Longevity in Model Organisms
- Proteoglycans and glycosaminoglycans research
- Vector-borne infectious diseases
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Immunotherapy and Immune Responses
- Chronic Lymphocytic Leukemia Research
- Image Enhancement Techniques
- Metabolism, Diabetes, and Cancer
- Protease and Inhibitor Mechanisms
- Acute Lymphoblastic Leukemia research
- Renal cell carcinoma treatment
- Cancer, Hypoxia, and Metabolism
Medical University of Vienna
2024
Institute of Molecular Biotechnology
2022-2024
Griffith University
2021-2024
Austrian Academy of Sciences
2022-2024
University of Michigan
2024
University of Applied Sciences Upper Austria
2024
BOKU University
2024
University of British Columbia
2024
Fresti (Portugal)
2022-2023
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2016
Postoperative pain affects most patients after major surgery and can transition to chronic pain. The considerable side effects limited efficacy of current treatments underline the need for new therapeutic options. We observed increased amounts metabolites BH4 serotonin skin injury. Mast cells were primary postoperative sources Gch1 , rate-limiting enzyme in synthesis, itself an obligate cofactor production by tryptophan hydroxylase (Tph1). Mice deficient mast or cell–specific Tph1 showed...
Dysregulation of tumor suppressor protein E-cadherin is an early molecular event in cancer. O-mannosylation profile a newly-described post-translational modification crucial for its adhesive functions homeostasis. However, the role O-mannosyl glycans E-cadherin-mediated cell adhesion cancer and their interplay with N-glycans remains largely unknown. We herein demonstrated that human gastric carcinomas exhibiting non-functional display reduced expression concomitantly increased branched...
Solute carrier (SLC) transporters govern most of the chemical exchange across cellular membranes and are integral to metabolic regulation, which in turn is linked function identity. Despite their key role, individual functions members SLC superfamily were not evaluated systematically. We determined transcriptional profiles upon overexpression knock-out or wild-type isogenic cell backgrounds. Targeted metabolomics provided a fingerprint 189 intracellular metabolites, while transcriptomics...
B-cell precursor acute lymphoblastic leukemia (BCP-ALL) with mixed-lineage gene rearrangement (MLL-r) is a poor-prognosis subtype for which additional therapeutic targets are urgently needed. Currently no multi-omics data set primary MLL r patient cells exists that integrates transcriptomics, proteomics and glycomics to gain an inclusive picture of theranostic targets. Methods: We have integrated i) obtain the first BCP-ALL identify molecular signatures distinguish leukemic from normal...
N-glycosylation is one of the most common protein modifications in eukaryotes, with immense importance at molecular, cellular, and organismal level. Accurate reliable N-glycan analysis essential to obtain a systems-wide understanding fundamental biological processes. Due structural complexity glycans, their still highly challenging. Here we make publicly available consistent N-glycome dataset 20 different mouse tissues demonstrate multimodal data workflow that allows for unprecedented depth...
Integrin alpha 2 (ITGA2) promotes cancer metastasis through selective adhesion to ECM proteins; however, the specific contribution of integrin glycosylation remains uncertain. We provide evidence that ITGA2 is a highly glycosylated transmembrane protein expressed in ovarian tissue and cell lines. In-depth glycoproteomics identified predominant N- O-glycosylation sites harboring substantially divergent profiles. Generated putative N-glycosite mutants halted collagen laminin binding cells...
Liquid-based cytology (LBC) plays a crucial role in the effective early detection of cervical cancer, contributing to substantially decreasing mortality rates. However, visual examination microscopic slides is challenging, time-consuming, and ambiguous task. Shortages specialized staff equipment are increasing interest developing artificial intelligence (AI)-powered portable solutions support screening programs. This paper presents novel approach based on RetinaNet model with ResNet50...
Abstract C-type lectins are a large family of proteins with essential functions in both health and disease. In cancer, some have been found to promote inhibit tumor growth, but many the still remain uncharacterised context. Therefore, there is growing interst further elucidating mechanisms which control growth. Here, we report key role CLEC18 progression clear cell renal carcinoma (ccRCC). The conserved across entire Chordata phylum recent gene duplication events humans. We that CLEC18A...
Deep learning approaches require a large amount of data to be transferred centralized entities. However, this is often not feasible option in healthcare, as it raises privacy concerns over sharing sensitive information. Federated Learning (FL) aims address issue by allowing machine without transferring the entity. FL has shown great potential ensure digital healthcare while maintaining performance. Despite this, there lack research on impact different types heterogeneity results. In study,...
Glycosylation is the most common post-translational modification of proteins, and glycosylation changes at cell surfaces are frequently associated with malignant epithelia including head neck squamous carcinoma (HNSCC). In HNSCC, 5-year survival remains poor, averaging around 50% globally: this partly related to late diagnosis. Specific protein signatures on keratinocytes have promise as diagnostic prognostic biomarkers therapeutic targets. Nevertheless, HNSCC-specific glycome date largely...
Glycomics and sialiomics of isolated synaptosomes reveal distinct glycosylation surface proteins localized in the active zone synapses.
Microscopic techniques in low-to-middle income countries are constrained by the lack of adequate equipment and trained operators. Since light microscopy delivers crucial methods for diagnosis screening numerous diseases, several efforts have been made scientific community to develop low-cost devices such as 3D-printed portable microscopes. Nevertheless, these present some drawbacks that directly affect image quality: capture samples is done via mobile phones; more affordable lenses usually...
Postoperative pain affects most patients after major surgery and can transition to chronic pain. Here, we discovered that postoperative hypersensitivity correlated with markedly increased local levels of the metabolite BH4. Gene transcription reporter mouse analyses skin injury identified neutrophils, macrophages mast cells as primary sources GTP cyclohydrolase-1 (
N-glycosylation is one of the most common protein modifications in eukaryotes, with immense importance at molecular, cellular, and organismal level. Accurate reliable N-glycan analysis essential to obtain a systems-wide understanding fundamental biological processes. Due structural complexity glycans, their still highly challenging. Here we make publicly available consistent N-glycome dataset 20 different mouse tissues demonstrate multimodal data workflow that allows for unprecedented depth...
Abstract Clear cell renal carcinoma (ccRCC) is the kidney malignancy with highest incidence and mortality rates. Despite high patient burden, there are no biomarkers for rapid diagnosis public health surveillance. Urine would be an ideal source of ccRCC due to low invasiveness, easy accessibility, kidney’s intrinsic role in filtering urine. In present work, by combining proteomics, lipidomics metabolomics, we detected urogenital metabolic dysregulation patients increased lipid metabolism,...
Reduced responsiveness of precursor B-acute lymphoblastic leukemia (BCP-ALL) to chemotherapy can be first detected in the form minimal residual disease cells that persist after 28 days initial treatment. The ability these resist is partly due microenvironment bone marrow, which promotes cell growth and provides protection, particularly under conditions stress. It unknown if how glycocalyx such remodelled during development tolerance drug treatment, even though glycosylation most abundant...
Breast cancer is the most common among women. However, use of immune checkpoint inhibitors, that have revolutionized treatment multiple cancers, unfortunately remain largely ineffective in breast patients. Here, we report comprehensive glycoproteome map tumor cells, pointing to a key role sialic acid modifications mammary cancer. Genetic and pharmacologic inhibition sialylation repolarizes microenvironment, leading reduction myeloid-derived suppressor cells significant increase Tcf7+ memory...
Summary N-glycosylation is one of the most common protein modifications in eukaryotes, with immense importance at molecular, cellular, and organismal level. Accurate reliable N-glycan analysis essential to obtain a systems-wide understanding fundamental biological processes. Due structural complexity glycans, their still highly challenging. Here we make publicly available consistent N-glycome dataset 20 different mouse tissues demonstrate multimodal data workflow that allows for...
Abstract B-cell precursor acute lymphoblastic leukemia (BCP-ALL) with mixed-lineage gene rearrangement (MLL-r) is a poor-prognosis subtype for which additional therapeutic targets are urgently needed. Currently no multi omics data set primary MLL r patient cells exists that integrates transcriptomics, proteomics and glycomics to gain an inclusive picture of theranostic targets. Methods We have integrated i) obtain the first BCP-ALL identify molecular signatures distinguish leukemic from...