A5050811775- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Influenza Virus Research Studies
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Protein Structure and Dynamics
- Clostridium difficile and Clostridium perfringens research
- Viral Infections and Immunology Research
- Peptidase Inhibition and Analysis
- Bacteriophages and microbial interactions
- Erythrocyte Function and Pathophysiology
- Microbial Natural Products and Biosynthesis
- Ubiquitin and proteasome pathways
- Coccidia and coccidiosis research
- Capital Investment and Risk Analysis
- Cell Adhesion Molecules Research
- Eosinophilic Disorders and Syndromes
- Hepatitis Viruses Studies and Epidemiology
- Antifungal resistance and susceptibility
- Genomics and Phylogenetic Studies
- Protein purification and stability
- Cancer-related gene regulation
- Antimicrobial Resistance in Staphylococcus
- Fungal and yeast genetics research
- Carbohydrate Chemistry and Synthesis
Griffith University
2011-2024
Alten (Italy)
2019
University of Milan
2017
Tecnologie Avanzate (Italy)
1999-2001
Fondazione IRCCS Istituto Nazionale dei Tumori
1995
Histo-blood group antigens (HBGAs) have been proposed as rotavirus receptors. H type-1 and Lewisb reported to bind VP8* from major human genotypes P[4], P[6] P[8], while a rarer P[14] recognizes A-type HBGAs. However, the role significance of HBGA receptors in pathogenesis remains uncertain. Here we report that HAL1166 related P[9] K8 HBGAs, although neither virus engages HBGA-specific α1,2-linked fucose moiety. Notably, rotaviruses DS-1 (P[4]) RV-3 (P[6]) also use HBGAs for infection, with...
CD52, a glycophosphatidylinositol (GPI)-anchored glycoprotein, is released in soluble form following T cell activation and binds to the Siglec (sialic acid-binding Ig-like lectin)-10 receptor on cells suppress their function. We show that binding of CD52-Fc Siglec-10 suppression requires damage-associated molecular pattern (DAMP) protein, high-mobility group box 1 (HMGB1). bound specifically proinflammatory Box B domain HMGB1, this turn promoted CD52 N-linked glycan, α-2,3 sialic acid...
The gene slpA, encoding the S-layer precursor protein in virulent Clostridium difficile strains C253 and 79--685, was identified. carries a C-terminal highly conserved anchoring domain, similar to one found Cwp66 adhesin (previously characterized strain 79--685), an SLH variable N-terminal domain mediating cell adherence. genes proteins are present genetic locus carrying 17 open reading frames, 11 of which encode two-domain architecture, likely include surface-anchored proteins.
Novel 3-C-alkylated-Neu5Ac2en derivatives have been designed to target the expanded active site cavity of influenza virus sialidases with an open 150-loop, currently seen in X-ray crystal structures A group-1 (N1, N4, N5, N8), but not group-2 (N2, N9), sialidases. The compounds show selectivity for inhibition H5N1 and pdm09 H1N1 over N2 sialidase, providing evidence relative 150-loop flexibility these In a complex N8 C3 substituent 3-phenylally-Neu5Ac2en occupies 150-cavity while central...
Influenza virus infection continues to cause significant, often severe, respiratory illness worldwide. A validated target for the development of anti-influenza agents is surface protein sialidase. In current study, we have discovered a highly potent inhibitor influenza sialidase, based on novel sialosyl sulfonate template. The synthesised 3-guanidino α-sulfonate, sulfonozanamivir analogue, inhibits viral replication in vitro at nanomolar level, comparable that drug zanamivir. Using X-ray...
Enterovirus 71 (EV71) is a major etiological agent of hand, foot, and mouth disease, for which there no antiviral therapy. We have developed densely sulfated disaccharide heparan sulfate (HS) analogues that are potent small molecule inhibitors EV71 infection, binding to the viral capsid acting as decoy receptors block early events virus replication. The simplified structures, more than defined HS disaccharides with significant anticoagulant activity, offer promise anti-EV71 agents.
Abstract Siglec-2 undergoes constitutive endocytosis and is a drug target for autoimmune diseases B cell-derived malignancies, including hairy cell leukaemia, marginal zone lymphoma, chronic lymphocytic leukaemia non-Hodgkin’s lymphoma (NHL). An alternative to current antibody-based therapies the use of liposomal nanoparticles loaded with cytotoxic drugs decorated ligands. We have recently designed first ligands (9-biphenylcarboxamido-4- meta -nitrophenyl-carboxamido-Neu5Acα2Me, 9-BPC-4- m...
CMP-sialic acid transporter: We report an in-depth, multidisciplinary, structural study that has identified the amino residues intimately involved in transporter (CST) substrate specificity. Our data provide a significant contribution towards better understanding structure-function relationship of this important family transporters and rational design CST inhibitors.
Surface layers (S-layers) are regularly ordered protein subunits found as the outermost cell envelope component of many bacteria. Most S-layers composed a single or glycoprotein species with molecular weight varying between 40 and 200 kDa. Clostridium difficile is most common cause antibiotic associated diarrhea (AAD) pseudomembranous colitis (PMC) in humans. Detection S-layer some C. strains, preliminary characterization two glycoproteins, P36 P47, involved composition one these strains (C....
Fibrillarin (FBL) is an essential and evolutionarily highly conserved S-adenosyl methionine (SAM) dependent methyltransferase. It the catalytic component of a multiprotein complex that facilitates 2'-O-methylation ribosomal RNAs (rRNAs), modification for accurate efficient protein synthesis in eukaryotic cells. was recently established human FBL (hFBL) critical Nipah, Hendra, respiratory syncytial virus infections. In addition, overexpression hFBL contributes towards tumorgenesis associated...