A5071612952- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and biosynthesis
- Advanced Chemical Sensor Technologies
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- RNA modifications and cancer
- Signaling Pathways in Disease
- Mitochondrial Function and Pathology
- Folate and B Vitamins Research
- Spectroscopy and Chemometric Analyses
- Liver Disease Diagnosis and Treatment
- ATP Synthase and ATPases Research
- Cardiovascular Disease and Adiposity
- HIV-related health complications and treatments
- Identification and Quantification in Food
- Renal cell carcinoma treatment
- Autophagy in Disease and Therapy
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Alcohol Consumption and Health Effects
- Coconut Research and Applications
- RNA Research and Splicing
- Pancreatic function and diabetes
- Neurogenetic and Muscular Disorders Research
- Plant biochemistry and biosynthesis
University of Graz
2022-2025
Nawi Graz
2024
Medical University of Graz
2018-2023
University Hospital of Zurich
2023
University of Zurich
2023
Graz University of Technology
2023
ZHAW Zurich University of Applied Sciences
2010
Respiratory complex I is a redox-driven proton pump, accounting for large part of the electrochemical gradient that powers mitochondrial adenosine triphosphate synthesis. Complex dysfunction associated with severe human diseases. Assembly one-megadalton in inner membrane requires assembly factors and chaperones. We have determined structure from aerobic yeast Yarrowia lipolytica by electron cryo-microscopy at 3.2-Å resolution. A ubiquinone molecule was identified access path to active site....
Nonalcoholic fatty liver disease (NAFLD) is a common metabolic dysfunction leading to hepatic steatosis. However, NAFLD's global impact on the lipidome poorly understood. Using high-resolution shotgun mass spectrometry, we quantified molar abundance of 316 species from 22 major lipid classes in biopsies 365 patients, including nonsteatotic patients with normal or excessive weight, diagnosed NAFL (nonalcoholic liver) NASH steatohepatitis), and bearing mutations NAFLD-related protein factors....
Hepatic triacylglycerol accumulation and insulin resistance are key features of NAFLD. However, NAFLD development progression rather triggered by the aberrant generation lipid metabolites signaling molecules including diacylglycerol (DAG) lysophosphatidylcholine (lysoPC). Recent studies showed decreased expression carboxylesterase 2 (CES2) in liver NASH patients hepatic DAG was linked to low CES2 activity obese individuals. The mouse genome encodes several Ces2 genes with Ces2a showing...
Significance Phosphoenolpyruvate carboxykinase (PEPCK) has a unique position in cell metabolism by allowing the flexible use of nutrients for anabolic biosynthetic reactions. PEPCK activity just recently been described cancer cells. It shown to promote survival under nutrient deprivation, typical feature solid cancers, as well growth. To decipher metabolic downstream pathways is critical better understanding adaptive responses low stress. Here, we show that mitochondrial (PEPCK-M) mediates...
Chronically elevated circulating fatty acid levels promote lipid accumulation in nonadipose tissues and cause lipotoxicity. Adipose triglyceride lipase (ATGL) critically determines the release of acids from white adipose tissue, accumulating evidence suggests that inactivation ATGL has beneficial effects on lipotoxicity-driven disorders including insulin resistance, steatohepatitis, heart disease, classifying as a promising drug target. Here, we report development biological characterization...
Rett syndrome (RTT) is defined as a rare disease caused by mutations of the methyl-CpG binding protein 2 (MECP2). It one most common causes genetic mental retardation in girls, characterized normal early psychomotor development, followed severe neurologic regression. Hitherto, RTT lacks specific biomarker, but altered lipid homeostasis has been found model mice well patients. We performed LC-MS/MS lipidomics analysis to investigate cerebrospinal fluid (CSF) and plasma composition patients...
Physical inactivity is one of the leading causes chronic metabolic disease including obesity. Increasing physical activity (PA) has been shown to improve cardiometabolic and musculoskeletal health be associated with a distinct gut microbiota composition in trained athletes. However, impact PA on inconclusive for individuals performing their day-to-day life. This study examined role hand-grip strength microbiome middle-aged adults (40-65 years, n = 350) normal (18.5-24.9 kg/m2 ) overweight...
Abstract Spinal muscular atrophy (SMA) is a rare neuromuscular disease caused by biallelic mutations in the SMN1 gene, leading to progressive muscle weakness due degeneration of anterior horn cells. Since 2017, SMA patients can be treated with anti-sense oligonucleotide Nusinersen, which promotes alternative splicing SMN2 regular intrathecal injections. In this prospective study, we applied metabolomic, lipidomic, and proteomic analysis examine sequential CSF samples from 13 controls. This...
In the highly dynamic field of metabolomics, we have developed a method for analysis hydrophilic metabolites in various biological samples. Therefore, used interaction chromatography (HILIC) separation, combined with high-resolution mass spectrometer (MS) aim separating and analyzing wide range compounds. We 41 reference standards different chemical properties to develop an optimal chromatographic separation. MS was performed set pooled samples human cerebrospinal fluid (CSF), plasma. The...
Matching both the construction of a recombinant strain and process design with characteristics target protein has potential to significantly enhance bioprocess performance, robustness, reproducibility. The factors affecting physiological state Pichia pastoris Mut(+) (methanol utilization-positive) strains their cell membranes were quantified at individual level using combination staining fluorescent dyes flow cytometric enumeration. Cell vitalities found range from 5 95% under various...
Styrian pumpkin seed oil is a conditioned green-colored renowned for nutty smell and taste. Due to α-linolenic acid (ALA) contents below 1% of total fatty acids the prospect nutritional health claims based on its potential oxidation products, we investigated fate ALA product oxylipins in course down-stream processing seeds oils. Lipidomic analyses with Lipid Data Analyzer 2.8.1 revealed: Processing did not change (1) main composition oils, (2) amounts triacylglycerol species, (3) structures...
The activation of stress response pathways in synovial fibroblasts (SF) is a hallmark rheumatoid arthritis (RA). CBP and p300 are two highly homologous histone acetyl transferases writers activating 3 lysine 27 acetylation (H3K27ac) marks. Furthermore, they serve as co-factors for transcription factors acetylate many non-histone proteins. Here we showed that but not protein expression was down regulated by TNF 4-hydroxynonenal, mimic inflammation oxidative the microenvironment. We used...
Atherosclerosis, the leading cause of cardiovascular disease, cannot be sufficiently explained by established risk factors, including cholesterol. Elevated plasma homocysteine (Hcy) is an independent factor for atherosclerosis and closely linked to mortality. However, its role in has not been fully clarified yet. We have previously shown that rabbits fed a diet deficient B vitamins choline (VCDD), which are required Hcy degradation, exhibit accumulation macrophages lipids aorta, aortic...
Calibration is an important source of variability in liquid chromatography mass spectrometry (LC-MS) methods for insulin-like growth factor 1 (IGF-1). This study investigated the impact different calibrator matrices on IGF-1 measurements by LC-MS. Moreover, comparability immunoassays and LC-MS was assessed.Calibrators from 12.5 to 2009 ng/ml were prepared spiking WHO international Standard (ID 02/254 NIBSC, UK) into following matrices: native human plasma, fresh charcoal-treated plasma...
Abstract Clear cell renal carcinoma (ccRCC) is the kidney malignancy with highest incidence and mortality rates. Despite high patient burden, there are no biomarkers for rapid diagnosis public health surveillance. Urine would be an ideal source of ccRCC due to low invasiveness, easy accessibility, kidney’s intrinsic role in filtering urine. In present work, by combining proteomics, lipidomics metabolomics, we detected urogenital metabolic dysregulation patients increased lipid metabolism,...
Abstract Aims Carotenoids are a class of hydrophobic substances that important as food and feed colorants antioxidants. The pathway for β-carotene synthesis has been expressed in various yeast species, albeit with rather low yields titers. inefficient conversion phytoene to lycopene is often regarded bottleneck the pathway. In this study, we aimed at improvement production Saccharomyces cerevisiae by specifically engineering enzymatic reactions producing converting phytoene. Methods Results...
Monoglyceride lipase (MGL) hydrolyzes monoacylglycerols (MG) to glycerol and one fatty acid. Among the various MG species, MGL also degrades 2-arachidonoylglycerol, most abundant endocannabinoid potent activator of cannabinoid receptors 1 2. We investigated consequences deficiency on platelet function using systemic (Mgl−/−) platelet-specific Mgl-deficient (platMgl−/−) mice. Despite comparable morphology, loss was associated with decreased aggregation reduced response collagen activation....
Abstract Atherosclerosis, the leading cause of cardiovascular disease, cannot be sufficiently explained by established risk factors such as cholesterol. Elevated plasma homocysteine (Hcy) is an independent factor for atherosclerosis and closely linked to mortality. However, its role in has not been fully clarified. We have previously shown that rabbits fed a diet deficient B vitamins choline (VCDD), required Hcy degradation, exhibit accumulation macrophages lipids aorta, impairment...
Individual functions of members the bromodomain (BRD) and extra-terminal (BET) protein family underlying anti-inflammatory effects BET inhibitors in rheumatoid arthritis (RA) are incompletely understood. Here, we aimed to analyze regulatory BRD3, an understudied member family, RA synovial fibroblasts (FLS). BRD3 was silenced FLS prior stimulation with TNF. Alternatively, were treated I-BET. Transcriptomes analyzed by RNA sequencing (RNAseq), followed pathway enrichment analysis. We confirmed...