A5068088446- Pancreatic function and diabetes
- Lipid metabolism and biosynthesis
- Cellular transport and secretion
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Lysosomal Storage Disorders Research
- Cannabis and Cannabinoid Research
- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Glycosylation and Glycoproteins Research
- Autophagy in Disease and Therapy
- Carbohydrate Chemistry and Synthesis
- Calcium signaling and nucleotide metabolism
- Biochemical and Molecular Research
- Lipid metabolism and disorders
University of Graz
2020-2024
Graz University of Technology
2016
Chronically elevated circulating fatty acid levels promote lipid accumulation in nonadipose tissues and cause lipotoxicity. Adipose triglyceride lipase (ATGL) critically determines the release of acids from white adipose tissue, accumulating evidence suggests that inactivation ATGL has beneficial effects on lipotoxicity-driven disorders including insulin resistance, steatohepatitis, heart disease, classifying as a promising drug target. Here, we report development biological characterization...
Abstract Bis(monoacylglycero)phosphate (BMP) is a major phospholipid constituent of intralumenal membranes in late endosomes/lysosomes, where it regulates the degradation and sorting lipid cargo. Recent observations suggest that Batten disease - associated protein CLN5 functions as lysosomal BMP synthase. Here, we show transacylation reactions catalyzed by cytosolic secreted enzymes enhance synthesis independently CLN5. The transacylases identified this study are capable acylating precursor...
To date, the only enzyme known to be responsible for hydrolysis of cholesteryl esters and triacylglycerols in lysosome at acidic pH is lysosomal acid lipase (LAL). Lipid malabsorption small intestine (SI), accompanied by macrophage infiltration, one most common pathological features LAL deficiency. However, exact role intestinal lipid metabolism still unknown. We collected three parts SI (duodenum, jejunum, ileum) from mice with a global (LAL KO) or intestine-specific deletion (iLAL...
Abstract Biomimetic synthesis routes towards the important natural d ‐mannosyl donor guanosine 5′‐diphospho‐ ‐mannose (GDP‐Man) rely on kinase‐catalyzed nucleotide triphosphate (NTP)‐dependent phosphorylations of (Man), to give 6‐phosphate or α‐ 1‐phosphate (αMan 1‐P) as an intermediate product. A GDP‐Man not requiring kinase/NTP system would be practical and cost‐effective. Here, we have developed a multienzyme cascade GDP‐Man, characterized in that αMan 1‐P was obtained by...
Bis(monoacylglycero)phosphate (BMP), also known as lysobisphosphatidic acid, is a phospholipid that promotes lipid sorting in late endosomes/lysosomes by activating hydrolases and transfer proteins. Changes the cellular BMP content therefore reflect an altered metabolic activity of endolysosomal system. Surprisingly, little about physiological regulation BMP. In this study, we investigated effects nutritional factors on profiles whole tissues parenchymal nonparenchymal cells. Tissue samples...
Bis(monoacylglycero)phosphate (BMP) is a major phospholipid constituent of intralumenal membranes in late endosomes/lysosomes, where it regulates the degradation and sorting lipid cargo. Recent observations suggest that Batten disease-associated protein CLN5 functions as lysosomal BMP synthase. Here, we show transacylation reactions catalyzed by cytosolic secreted enzymes enhance synthesis independently CLN5. The transacylases identified this study are capable acylating precursor...