A5073748004- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Developmental Biology and Gene Regulation
- Retinal Development and Disorders
- RNA regulation and disease
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Chromatin Remodeling and Cancer
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
- Neurogenesis and neuroplasticity mechanisms
- Hippo pathway signaling and YAP/TAZ
- Fetal and Pediatric Neurological Disorders
- RNA modifications and cancer
- Photoacoustic and Ultrasonic Imaging
- Cerebrospinal fluid and hydrocephalus
- Congenital heart defects research
- Cell Image Analysis Techniques
- CRISPR and Genetic Engineering
- Retinal and Macular Surgery
- Proteoglycans and glycosaminoglycans research
- 3D Printing in Biomedical Research
- Celiac Disease Research and Management
- Animal Genetics and Reproduction
- Axon Guidance and Neuronal Signaling
Institute of Molecular Biotechnology
2019-2024
Austrian Academy of Sciences
2022-2024
Max Planck Institute of Molecular Cell Biology and Genetics
2016-2022
TU Dresden
2016-2017
Tata Institute of Fundamental Research
2014-2016
Max Planck Society
2015
Organ formation is a multi-scale event that involves changes at the intracellular, cellular and tissue level. Organogenesis often starts with of characteristically shaped organ precursors. However, mechanisms driving precursor are not clear. Here, using zebrafish, we investigate epithelial rearrangements responsible for development hemispherical retinal neuroepithelium (RNE), part optic cup. We show in addition to basal shrinkage RNE cells, active migration connected cells into crucial...
The establishment and maintenance of apical-basal polarity is a fundamental step in brain development, instructing the organization neural progenitor cells (NPCs) developing cerebral cortex. Particularly, basally located extracellular matrix (ECM) crucial for this process. In vitro, epithelial polarization can be achieved via endogenous ECM production, or exogenous supplementation. While neuroepithelial development recapitulated organoids, effects different sources tissue morphogenesis...
Mutations in ARID1B, a member of the mSWI/SNF complex, cause severe neurodevelopmental phenotypes with elusive mechanisms humans. The most common structural abnormality brain ARID1B patients is agenesis corpus callosum (ACC), characterized by absence an interhemispheric white matter tract that connects distant cortical regions. Here, we find neurons expressing SATB2, determinant callosal projection neuron (CPN) identity, show impaired maturation ARID1B+/− neural organoids. Molecularly,...
During development of the human cerebral cortex, multipotent neural progenitors generate excitatory neurons and glial cells. Investigations transcriptome epigenome have revealed important gene regulatory networks underlying this crucial developmental event. However, posttranscriptional control expression protein abundance during corticogenesis remains poorly understood. We addressed issue by using telencephalic brain organoids grown a dual reporter cell line to isolate performed class...
Light sheet fluorescence microscopy (LSFM) is gaining more and popularity as a method to image embryonic development. The main advantages of LSFM compared confocal systems are its low phototoxicity, gentle mounting strategies, fast acquisition with high signal noise ratio the possibility imaging samples from various angles (views) for long periods time. Imaging multiple views unleashes full potential LSFM, but at same time it can create terabyte-sized datasets. Processing such datasets...
The epidermis is a stratified epithelium, which forms barrier to maintain the internal milieu in metazoans. Being outermost tissue, growth of has be strictly coordinated with embryo. key parameters that determine tissue are cell number and size. So far, it remained unclear how size epidermal cells maintained whether contributes towards homeostasis. We have used genetic analysis combination cellular imaging show zebrafish goosepimples/myosin Vb regulates plasma membrane homeostasis involved...
Microvillus inclusion disease (MVID) is a life-threatening enteropathy characterised by malabsorption and incapacitating fluid loss due to chronic diarrhoea. Histological analysis has revealed that enterocytes in MVID patients exhibit reduction of microvilli, presence microvillus bodies intestinal villus atrophy, whereas genetic linkage identified mutations myosin Vb gene as the main cause MVID. In order understand cellular basis associated formation bodies, an animal model develops ex utero...
Light sheet fluorescence microscopy (LSFM) is gaining more and popularity as a method to image embryonic development. The main advantages of LSFM compared confocal systems are its low phototoxicity, gentle mounting strategies, fast acquisition with high signal noise ratio the possibility imaging samples from various angles (views) for long periods time. Imaging multiple views unleashes full potential LSFM, but at same time it can create terabyte-sized datasets. Processing such datasets...
Abstract Establishment and maintenance of apical-basal polarity is a fundamental step in brain development, instructing the organization neural progenitor cells (NPCs) developing cerebral cortex. Particularly, basally located extracellular matrix (ECM) crucial for this process. In vitro, epithelial polarization can be achieved via endogenous ECM production, or exogenous supplementation. While neuroepithelial development recapitulated organoids, effects different sources tissue morphogenesis...
Abstract Mutations in ARID1B , a member of the mSWI/SNF complex, cause severe neurodevelopmental phenotypes with elusive mechanisms humans. The most common structural abnormality brain patients is agenesis corpus callosum (ACC). This condition characterized by partial or complete absence (CC), an interhemispheric white matter tract that connects distant cortical regions. Using human neural organoids, we identify vulnerability callosal projection neurons (CPNs) to haploinsufficiency,...
Abstract The expansion of the mammalian brain is associated with specific developmental processes; however, not much known about how evolutionary changes participated in acquisition human traits during early stages. Here we investigated whether enhancers active phylotypic stage show human-specific genomic divergence which could contribute to forebrain. Notably, identified an enhancer containing a accelerated region (HAR) located Chromosome 14q12, enriched neurodevelopmental genes, such as...
Abstract Diverse modes of cell migration shape organisms in health and disease much research has focused on the role intracellular extracellular components different phenomena. What is less explored, however, how arrangement underlying matrix that many cells move upon vivo influences migration. Combining novel transgenic lines image analysis pipelines, reveals during zebrafish optic cup formation use cryptopodia-like protrusions to migrate collectively actively over a topologically changing...
During development of the human cerebral cortex, multipotent neural progenitors generate excitatory neurons and glial cells. Investigations transcriptome epigenome have revealed important gene regulatory networks underlying this crucial developmental event. However, post-transcriptional control expression protein abundance during corticogenesis remains poorly understood. We addressed issue by using telencephalic brain organoids grown a dual reporter cell line to isolate performed class...
Abstract Organ formation is a multi-scale event that involves changes at the intracellular, cellular and tissue level. Organogenesis often starts with of characteristically shaped organ precursors. However, mechanisms driving precursor are not clear. Here, using zebrafish, we investigate epithelial rearrangements responsible for development hemispherical retinal neuroepithelium (RNE), part optic cup. We show in addition to basal shrinkage RNE cells, active migration connected cells into...
Background: Microvillus inclusion disease (MVID) is a life threatening enteropathy characterised by perpetual diarrhoea. Recent analysis has revealed that enterocytes in MVID patients exhibit reduction of microvilli, presence microvillus bodies and intestinal villus atrophy. Genetic linkage identified mutations myosin Vb gene as the main cause MVID. An animal model develops ex-utero tractable genetically well microscopy would be highly useful to study cellular basis formation bodies....