A5008029658- Cervical Cancer and HPV Research
- Cancer Genomics and Diagnostics
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Biotin and Related Studies
- Healthcare Systems and Practices
- Cellular transport and secretion
- Health, Medicine and Society
- Microtubule and mitosis dynamics
- Autophagy in Disease and Therapy
- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Advances in Oncology and Radiotherapy
- Innovations in Medical Education
- Endoplasmic Reticulum Stress and Disease
- Renal and related cancers
- Ubiquitin and proteasome pathways
- Cerebrovascular and genetic disorders
- Hematopoietic Stem Cell Transplantation
- Signaling Pathways in Disease
- Social Policies and Family
- S100 Proteins and Annexins
- Immune Cell Function and Interaction
- Protein Degradation and Inhibitors
- CRISPR and Genetic Engineering
Inserm
2020-2025
Université de Lille
2020-2025
Centre Hospitalier Universitaire de Lille
2020-2025
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2020-2025
Princess Margaret Cancer Centre
2015-2024
University Health Network
2015-2024
Lille University Management
2024
Centre Hospitalier Universitaire de Poitiers
2018-2023
Université de Poitiers
2018-2023
Centre Hospitalier Universitaire de Tours
2016-2021
The identification of ubiquitin E3 ligase substrates has been challenging, due in part to low-affinity, transient interactions, the rapid degradation targets and inability identify proteins from poorly soluble cellular compartments. SCFβ-TrCP1 SCFβ-TrCP2 are well-studied ligases that target for proteasomal degradation, play important roles Wnt, Hippo, NFκB signaling. Combining 26S proteasome inhibitor (MG132) treatment with proximity-dependent biotin labeling (BioID) semiquantitative mass...
Zika virus (ZIKV) is a membrane enveloped Flavivirus with positive strand RNA genome, transmitted by Aedes mosquitoes. The geographical range of ZIKV has dramatically expanded in recent decades resulting increasing numbers infected individuals, and the spike infections been linked to significant increases both Guillain-Barré syndrome microcephaly. Although large number host proteins have physically and/or functionally other Flaviviruses, very little known about virus-host protein...
Carbonic anhydrase IX (CAIX) is a hypoxia inducible factor 1-induced, cell surface pH regulating enzyme with an established role in tumor progression and clinical outcome. However, the molecular basis of CAIX-mediated remains unclear. Here, we have utilized proximity dependent biotinylation (BioID) to map CAIX 'interactome' breast cancer cells order identify physiologically relevant CAIX-associating proteins potential roles progression. High confidence identified include metabolic...
Centriolar satellites are small electron-dense granules that cluster in the vicinity of centrosomes. Satellites have been implicated multiple critical cellular functions including centriole duplication, centrosome maturation, and ciliogenesis, but their precise composition assembly properties remained poorly explored. Here, we perform vivo proximity-dependent biotin identification (BioID) on 22 human satellite proteins, to identify 2,113 high-confidence interactions among 660 unique...
Abstract The worldwide SARS-CoV-2 outbreak poses a serious challenge to human societies and economies. proteins orchestrate complex pathogenic mechanisms that underlie COVID-19 disease. Thus, understanding how viral polypeptides rewire host protein networks enables better-founded therapeutic research. In complement existing proteomic studies, in this study we define the first proximal interaction network of proteins, at whole proteome level cells. Applying proximity-dependent biotinylation...
Salmonella utilizes a type 3 secretion system to translocate virulence proteins (effectors) into host cells during infection
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Abstract The skin is exposed to viral pathogens, but whether they contribute the oncogenesis of cancers has not been systematically explored. Here we investigated 19 tumor types by analyzing off-target reads from commonly available next-generation sequencing data for pathogens. We identified human papillomavirus 42 (HPV42) in 96% (n = 45/47) digital papillary adenocarcinoma (DPA), an aggressive cancer occurring on fingers and toes. show that HPV42, so far considered a nononcogenic,...
Lung cancer is the leading cause of mortality worldwide, with squamous cell carcinoma (SQCC) being second most common form. SQCCs are thought to originate in bronchial basal cells through an injury response smoking, which results this stem population committing hyperplastic rather than mucinous and ciliated fates. Copy number gains SOX2 region 3q26–28 occur 94% SQCCs, appear act both early late disease progression by stabilizing initial promoting growth invasive carcinoma. Thus, anti-SOX2...
Depolarized mitochondria can be degraded via mitophagy, a selective form of autophagy. The RAB GTPase RAB7A was recently shown to play key role in this process. regulates late endocytic trafficking under normal growth conditions but is translocated the mitochondrial surface following depolarization. However, how activity regulated during mitophagy not understood. Here, using proximity-dependent biotinylation approach (miniTurbo), we identified C5orf51 as specific interactor GDP-locked RAB7A....
Abstract An accurate spatial representation of protein-protein interaction networks is needed to achieve a realistic and biologically relevant interactomes. Here, we leveraged the information included in Proximity-Dependent Biotin Identification (BioID) interactomes SARS-CoV-2 proteins calculate weighted distances model organization SARS-CoV-2-human interactome three dimensions (3D) within cell-like volume. Cell regions with viral occupancy were highlighted, along coordination exploiting...
There is limited data regarding what motivations are behind the choice of oncology (both medical and radiation oncology) as a specialty among students. Therefore, aim this study to identify factors that motivate students choose specialty. Medical classes 2022 2023 in Universities Lille Amiens (North France) were enrolled quantitative online survey. Chi-square automatic interaction detection (CHAID) binary multiple linear regressions performed determine Among 563 respondents (response rate:...
Centrosome structure, function, and number are finely regulated at the cellular level to ensure normal mammalian development. Here, we characterize PPP1R35 as a novel bona fide centrosomal protein demonstrate that it is critical for centriole elongation. Using quantitative super-resolution microscopy mapping live-cell imaging show resident located in proximal lumen above cartwheel, region of has eluded detailed characterization. Loss function results decreased centrosome shortened centrioles...
Cilia and flagella are microtubule-based cellular projections with important sensory motility functions. Their absence or malfunction is associated a growing number of human diseases collectively referred to as ciliopathies. However, the fundamental mechanisms underpinning cilia biogenesis functions remain only partly understood. Here, we show that depleting LUZP1 its interacting protein, EPLIN, increases levels MyosinVa at centrosome primary formation. We further localizes both actin...
Abstract A number of unique proteases localize to specific sub‐compartments the mitochondria, but functions these enzymes are poorly defined. Here, in vivo proximity‐dependent biotinylation (BioID) is used map interactomes seven localized mitochondrial intermembrane space (IMS). In total, 802 high confidence proximity interactions with 342 proteins identified. While all co‐localized IMS markers OPA1 and CLPB, 230 interacting partners just one or two protease bait proteins, highlighting...
Abstract It has been recently shown that many proteins are lacking from reference databases used in mass spectrometry analysis, due to their translation templated on alternative open reading frames. This questions our current understanding of gene annotation and drastically expands the theoretical proteome complexity. The functions these (AltProts) still remain largely unknown. We have developed a large-scale unsupervised approach based cross-linking (XL-MS) followed by shotgun proteomics...
The Golgi-specific Brefeldin-A resistance factor 1 (GBF1) is the only large GEF that regulates Arf activation at
Maturation of lymphoid cells is controlled by the action stage and lineage-restricted transcription factors working in concert with general chromatin remodeling machinery to regulate gene expression. To better understand this functional interplay, we used Biotin Identification human embryonic kidney identify proximity interaction partners for GATA3, TCF7 (TCF1), SPI1, HLF, IKZF1, PAX5, ID1, ID2. The shared among included ARID1a, a BRG1-associated factor complex component. CUT&RUN analysis...
Merkel cell carcinoma (MCC) is an aggressive skin cancer with a high mortality rate. polyomavirus causes 80% of MCCs, encoding the viral oncogenes small T and truncated large (tLT) antigens. These proteins impair RB1-dependent G1/S checkpoint blockade subvert host epigenome to promote cancer. Whole-proteome analysis proximal interactomics identified tLT-dependent deregulation DNA damage response (DDR). Our investigation revealed, our knowledge, previously unreported interaction between tLT...
Abstract Salmonella utilizes translocated virulence proteins (termed effectors) to promote host cell invasion. The effector SopD contributes invasion by promoting scission of the plasma membrane, generating -containing vacuoles. is expressed in all lineages and plays important roles animal models infection, but its targets are unknown. Here we show that can bind inhibit small GTPase Rab10, through a C-terminal activating protein (GAP) domain. During Rab10 effectors MICAL-L1 EHBP1 recruited...