A5074766208- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Protein Tyrosine Phosphatases
- Protease and Inhibitor Mechanisms
- Mast cells and histamine
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Wnt/β-catenin signaling in development and cancer
- Cancer Genomics and Diagnostics
- HER2/EGFR in Cancer Research
- Cytokine Signaling Pathways and Interactions
- Galectins and Cancer Biology
- Signaling Pathways in Disease
- Cancer-related Molecular Pathways
- 14-3-3 protein interactions
- RNA modifications and cancer
- Cellular Mechanics and Interactions
- Lipid Membrane Structure and Behavior
- Proteoglycans and glycosaminoglycans research
- Melanoma and MAPK Pathways
- S100 Proteins and Annexins
Institut de Recherche en Cancérologie de Montpellier
2012-2024
Inserm
2012-2024
Université de Montpellier
2010-2024
Centre National de la Recherche Scientifique
2010-2024
Universitair Ziekenhuis Brussel
2023
Ghent University Hospital
1987-2023
Institut Regional du Cancer de Montpellier
2016-2017
Centre de Recherche en Biologie cellulaire de Montpellier
2008-2012
Centre National pour la Recherche Scientifique et Technique (CNRST)
2005-2012
La Ligue Contre le Cancer
2012
Sphingosine-1-phosphate (SPP), a bioactive lipid, acts both intracellularly and extracellularly to cause pleiotropic biological responses. Recently, we identified SPP as ligand for the G protein–coupled receptor Edg-1 (Lee, M.-J., J.R. Van Brocklyn, S. Thangada, C.H. Liu, A.R. Hand, R. Menzeleev, Spiegel, T. Hla. 1998. Science. 279:1552–1555). binds with remarkable specificity only sphinganine-1-phosphate displaced radiolabeled SPP, while other sphingolipids did not. Binding of resulted in...
Rearrangements of the anaplastic lymphoma kinase (ALK) gene in non-small cell lung cancer (NSCLC) represent a novel molecular target small subset tumors. Although ALK rearrangements are usually assessed by immunohistochemistry (IHC) and fluorescence situ hybridization (FISH), approaches have recently emerged as relevant alternatives routine laboratories. Here, we evaluated use two different amplicon-based next-generation sequencing (NGS) methods (AmpliSeq Archer®FusionPlex®) to detect...
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The aspartyl-protease cathepsin D (cath-D) is overexpressed and hypersecreted by epithelial breast cancer cells stimulates their proliferation. As tumor epithelial–fibroblast cell interactions are important events in progression, we investigated whether cath-D overexpression affects also fibroblast behavior. We demonstrate a requirement of for invasive growth using three-dimensional (3D) coculture assay with secreting or not pro-cath-D. Ectopic expression cath-D–deficient fibroblasts 3D...
The mechanisms and receptors involved in phagocytosis by nonhematopoietic cells are not well understood. involvement of the alpha 3 beta 1 integrin extracellular matrix human breast cancer was studied. possible role this suggested since but 2 subunits concentrated at membrane sites where local degradation fluorescently labeled gelatin occurs. Strikingly, anti-alpha monoclonal antibodies (mAbs) stimulate films, beads, Matrigel films a quantitative assay. Stimulation uptake mAb is dose...
Interactions between cancer cells and fibroblasts are crucial in progression. We have previously shown that the aspartic protease cathepsin D (cath-D), a marker of poor prognosis breast is overexpressed highly secreted by cells, triggers mouse embryonic fibroblast outgrowth via paracrine loop. Here, we show requirement cath-D for human mammary using three-dimensional co-culture assay with do or not secrete pro-cath-D. Interestingly, proteolytically-inactive pro-cath-D remains mitogenic,...
Abstract The effects of the anti‐estrogens 4‐hydroxytamoxifen (OHTam), ICI 164,384 and 182,780 were tested on MCF‐7/LCC2 breast‐carcinoma cell line, which grows significantly in presence OHTam serves as a model for studying anti‐estrogen resistance estrogen‐receptor‐positive breast cancer. Cell proliferation cathepsin‐D secretion strongly inhibited by either or alone combination with 17‐p‐estradiol (E2) OHTam. did not affect pS2 mRNA levels, but antagonized stimulatory E2 these 2 mRNAs. was...
Abstract We showed previously that the spleen tyrosine kinase Syk is expressed by mammary epithelial cells and it suppresses malignant growth of breast cancer cells. The exact molecular mechanism its tumor-suppressive activity remains, however, to be identified. Here, we show colocalizes copurifies with centrosomal component γ-tubulin exhibits a catalytic within centrosomes. Moreover, localization depends on intact activity. Centrosomal expression persistent in interphase but promptly drops...
The metastatic process requires changes in tumor cell adhesion properties, motility and remodeling of the extracellular matrix. erbB2 proto-oncogene is overexpressed approximately 30% breast cancers a major prognostic parameter when present invasive disease. A ligand for receptor has not yet been identified but it can be activated by heterodimerization with heregulin (HRG)-stimulated erbB3 erbB4 receptors. HRGs are family polypeptide growth factors that have shown to play role embryogenesis,...
In mammalian cells, three Cdc25 phosphatases A, B, C coordinate cell cycle progression through activating dephosphorylation of Cyclin-dependent kinases. Whereas Cdc25B is believed to trigger entry into mitosis, Cdc25C thought act at a later stage mitosis and in the nucleus. We report that fraction localises centrosomes cycle-dependent fashion, as late S phase throughout G(2) mitosis. Moreover, colocalises with Cyclin B1 prophase Fluorescence Recovery after Photobleaching experiments reveal...
Spheroidal cell aggregates were prepared from four tumorigenic human breast lines (HBL-100 and three MCF-7 variants). Cells these allowed to migrate towards lanes of basement membrane components coated on a glass substratum. Matrigel (reconstituted membrane) permanently arrested the migration one line, while others was permitted. Amongst several purified constituents only laminin, not collagen type IV or fibronectin, found cause same arrest migration. Within laminin molecule pepsin P1,...
Tangeretin is a methoxyflavone from citrus fruits, which inhibits growth of human mammary cancer cells and cytolysis by natural killer cells. Attempting to unravel the flavonoid's action mechanism, we found that it inhibited extracellular‐signal‐regulated kinases 1/2 (ERK1/2) phosphorylation in dose‐ time‐dependent way. In T47D this inhibition was optimally observed after priming with estradiol. The spectrum intracellular signalling kinase narrow comparison structural congeners showed ERK...
While first discovered in immunoreceptor signaling, the Syk protein kinase behaves as a tumor and metastasis suppressor epithelial cells. Its reduced expression breast other carcinomas is correlated with decreased survival increased risk, but its action mechanism remains largely unknown. Using phosphoproteomics we found that phosphorylated E-cadherin α-, β-, p120-catenins on multiple tyrosine residues concentrate at intercellular junctions. Increased activation enhanced E-cadherin/catenin...