A5047768373- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Porphyrin and Phthalocyanine Chemistry
- Photodynamic Therapy Research Studies
- Cellular Mechanics and Interactions
- Supramolecular Self-Assembly in Materials
- Cell Adhesion Molecules Research
- Chemical Synthesis and Analysis
- Cardiomyopathy and Myosin Studies
- Platelet Disorders and Treatments
- Nanoparticle-Based Drug Delivery
- Phytochemistry and biological activities of Ficus species
- Metal complexes synthesis and properties
- Lipid Membrane Structure and Behavior
- Muscle Physiology and Disorders
- Lanthanide and Transition Metal Complexes
- Essential Oils and Antimicrobial Activity
- Luminescence and Fluorescent Materials
- Magnetism in coordination complexes
- Nonlinear Optical Materials Studies
- Dendrimers and Hyperbranched Polymers
- Sesquiterpenes and Asteraceae Studies
- Supramolecular Chemistry and Complexes
- Monoclonal and Polyclonal Antibodies Research
Centre National de la Recherche Scientifique
2015-2025
Université de Montpellier
2015-2025
École Nationale Supérieure de Chimie de Montpellier
2015-2025
Institut des Biomolécules Max Mousseron
2016-2025
Université de Rennes
2023
Inserm
1990-2022
École Pratique des Hautes Études
2005-2022
École Polytechnique Fédérale de Lausanne
2021
Labex (Sweden)
2021
Bipar
2015-2020
The HIV-1 Tat protein is secreted by infected cells. Extracellular can affect bystander uninfected T cells and induce numerous biological responses such as apoptosis cytokine secretion. likely involved in several immune disorders during AIDS. Nevertheless, it not known whether triggers cell directly upon binding to signaling receptors at the plasma membrane or after delivery cytosol. pathway that enables reach cytosol also unclear. Here we visualized within T-cell-coated pits endosomes....
In addition to the already described ligand L4a, two pyclen-based lanthanide chelators, L4b and L4c, bearing specific picolinate two-photon antennas (tailor-made for each targeted metal) one acetate arm arranged in a dissymmetrical manner, have been synthesized, form complete family of luminescent bioprobes: [EuL4a], [SmL4a], [YbL4b], [TbL4c], [DyL4c]. Additionally, symmetrically regioisomer L4a′ was also synthesized as well its [EuL4a′] complex highlight astonishing positive impact...
Abstract Periodic mesoporous organosilica nanoparticles emerge as promising vectors for nanomedicine applications. Their properties are very different from those of well‐known silica there is no source their synthesis. So far, they have only been synthesized small bis‐silylated organic precursors. However, studies employing large stimuli‐responsive precursors reported on such hybrid systems yet. Here, the synthesis porphyrin‐based a octasilylated metalated porphyrin precursor described...
Dynamic covalent chemistry affords porphyrin–peptide cages which are found to be active photosensitizers for photodynamic therapy using green-light irradiation.
Interactions between cancer cells and fibroblasts are crucial in progression. We have previously shown that the aspartic protease cathepsin D (cath-D), a marker of poor prognosis breast is overexpressed highly secreted by cells, triggers mouse embryonic fibroblast outgrowth via paracrine loop. Here, we show requirement cath-D for human mammary using three-dimensional co-culture assay with do or not secrete pro-cath-D. Interestingly, proteolytically-inactive pro-cath-D remains mitogenic,...
We report periodic mesoporous ionosilica nanoparticles (PMINPs) as versatile nano-objects for imaging, photodynamic therapy (PDT), and efficient adsorption delivery of small interfering RNA (siRNA) into breast cancer cells. In order to endow these with PDT siRNA photochemical internalization (PCI) properties, a porphyrin derivative was integrated the framework. For this purpose, we synthesized PMINPs via hydrolysis-cocondensation procedures from oligosilylated ammonium precursors. The...
Biopolymers have significant pharmaceutical applications, and their blending has favorable characteristics for properties compared to the sole components. In this work, sodium alginate (SA) as a marine biopolymer was blended with poly(vinyl) alcohol (PVA) form SA/PVA scaffolds through freeze-thawing technique. Additionally, polyphenolic compounds in Moringa oleifera leaves were extracted by different solvents, it found that extracts 80% methanol had highest antioxidant activity. Different...
Inducing the degradation of pathological soluble antigens could be key to greatly enhancing efficacy therapeutic monoclonal antibodies (mAbs), extensively used in treatment autoimmune and inflammatory disorders or cancer. Lysosomal targeting has gained increasing interest recent years due its pharmaceutical applications far beyond lysosomal diseases, as a way address proteins lysosome for eventual degradation. Mannose 6-phosphonate derivatives (M6Pn), called AMFA, are unique glycovectors...
The concept of using two-photon excitation in the NIR for spatiotemporal control biological processes holds great promise. However, its use delivery nucleic acids has been very scarcely described and reported procedures are not optimal as they often involve potentially toxic materials irradiation conditions. This work prepares a simple system made biocompatible porous silicon nanoparticles (pSiNP) safe siRNA photocontrolled gene silencing cells upon excitation. PSiNP linked to an azobenzene...
Amphiphilic dynamic covalent polymer vectors formed in situ through RNA-templating deliver mRNA cells.
Abstract Bone regeneration is one of the most effective methods for treating bone defects. In this work, tricarboxylic cellulose/sodium alginate loaded with hydroxyapatite (HA) and/or graphene oxide (GO) was coagulated by calcium ions to create beads as scaffolds. first, cellulose oxidized water-soluble (TCC) 2,2,6,6‐tetramethylpiperidine-1-oxyl (TEMPO), periodate, and chlorite oxidation. HA extracted from eggshells via microwave treatment, GO synthesized using Hummer method. The structural...
Fragment self-assembly was used for producing clusters with a variety of scaffolds and ligands, an effective siRNA vector identified.
Flash photolysis of thermally stable, photolabile ‘caged’ precursors permits rapid and precise changes ligand concentration at their site action. This approach was used to determine the concentration‐dependence time course NO‐mediated relaxation aortic smooth muscle, by use two NO donors, trichloronitrosylruthenium (Ru(NO)Cl 3 ) dipotassium pentachloronitrosylruthenate (K 2 Ru(NO)Cl 5 ). At concentrations up 500 μ M , both compounds were non‐toxic before photolysis, produced by‐products on...
Dynamic covalent polymers made from modified amino acids complex nucleic and deliver siRNA in living cells.
While first discovered in immunoreceptor signaling, the Syk protein kinase behaves as a tumor and metastasis suppressor epithelial cells. Its reduced expression breast other carcinomas is correlated with decreased survival increased risk, but its action mechanism remains largely unknown. Using phosphoproteomics we found that phosphorylated E-cadherin α-, β-, p120-catenins on multiple tyrosine residues concentrate at intercellular junctions. Increased activation enhanced E-cadherin/catenin...
Dynamic covalent libraries enable exploring complex chemical systems from which bioactive assemblies can adaptively emerge through template effects. In this work, we studied dynamic made of complementary bifunctional cationic peptides, yielding a diversity species macrocycles to polymers. Although polymers are typically expressed only at high concentration, found that siRNA acts as in the formation low concentration process guided by electrostatic binding. Using glycosylated building block,...