A5101855407- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Protein Tyrosine Phosphatases
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- RNA and protein synthesis mechanisms
- Autophagy in Disease and Therapy
- Receptor Mechanisms and Signaling
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Protein Kinase Regulation and GTPase Signaling
- Electron Spin Resonance Studies
- Endoplasmic Reticulum Stress and Disease
- Advanced Biosensing Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Nuclear Structure and Function
- Cellular transport and secretion
- Analytical Chemistry and Sensors
- Cell death mechanisms and regulation
Université de Montpellier
2012-2024
Centre National de la Recherche Scientifique
2014-2024
Institut de Recherche en Cancérologie de Montpellier
2023-2024
Inserm
1999-2024
Institut de Génétique Moléculaire de Montpellier
2012-2022
La Ligue Contre le Cancer
2018-2021
MRC Protein Phosphorylation and Ubiquitylation Unit
2014
University of Dundee
2014
Centre de Recherche en Biologie cellulaire de Montpellier
2000-2012
King's College London
2003-2006
Single molecule FISH (smFISH) allows studying transcription and RNA localization by imaging individual mRNAs in single cells. We present smiFISH (single inexpensive FISH), an easy to use flexible visualization quantification approach that uses unlabelled primary probes a fluorescently labelled secondary detector oligonucleotide. The gene-specific are can therefore be synthesized at low cost, thus allowing more per mRNA resulting substantial increase detection efficiency. is also since...
Translation is an essential step in gene expression. In this study, we used improved SunTag system to label nascent proteins and image translation of single messenger ribonucleoproteins (mRNPs) human cells. Using a dedicated reporter RNA, observe that mRNPs stochastically turns on off while they diffuse through the cytoplasm. We further measure ribosome density 1.3 per kilobase elongation rate 13-18 amino acids second. Tagging endogenous POLR2A revealed similar rates ribosomal densities...
Cyclin A2 plays a key role in cell cycle regulation. It is essential embryonic cells and the hematopoietic lineage yet dispensable fibroblasts. In this paper, we demonstrate that A2–depleted display cortical distribution of actin filaments increased migration. These defects are rescued by restoration wild-type A2, which directly interacts with RhoA, or mutant unable to associate Cdk. vitro, potentiates exchange activity RhoA-specific guanine nucleotide factor. Consistent this, depletion...
Abstract Local translation allows for a spatial control of gene expression. Here, we use high-throughput smFISH to screen centrosomal protein-coding genes, and describe 8 human mRNAs accumulating at centrosomes. These localize different stages during cell cycle with remarkable choreography, indicating finely regulated translational program Interestingly, drug treatments reporter analyses reveal common translation-dependent localization mechanism requiring the nascent protein. Using ASPM...
While a significant amount is known about the biochemical signaling pathways of Rho family GTPase Cdc42, better understanding how these networks are coordinated in cells required. In particular, predominant subcellular sites where GTP-bound Cdc42 binds to its effectors, such as p21-activated kinase 1 (PAK1) and N-WASP, homolog Wiskott-Aldritch syndrome protein, still undetermined. Recent fluorescence resonance energy transfer (FRET) imaging experiments using activity biosensors show...
Abstract RNA localization is a crucial process for cellular function and can be quantitatively studied by single molecule FISH (smFISH). Here, we present an integrated analysis framework to analyze sub-cellular localization. Using simulated images, design validate set of features describing different patterns including polarized distribution, accumulation in cell extensions or foci, at the membrane nuclear envelope. These are largely invariant levels, work multiple lines, measure strength...
Invadosomes are F-actin-based structures involved in extracellular matrix degradation, cell invasion, and metastasis formation. Analyzing their proteome is crucial to decipher molecular composition, understand mechanisms, find specific elements target them. However, the analysis of invadosomes challenging, because it difficult maintain integrity during isolation. In addition, classical purification methods often suffer from contaminations, which may impair data validation. To ensure...
RNA localization and local translation are important for numerous cellular functions. In mammals, a class of mRNAs localize to cytoplasmic protrusions in an APC-dependent manner, with roles during cell migration. Here, we investigated this mechanism. We found that the KIF1C motor interacts is required their localization. Live imaging revealed rapid, active transport single over long distances requires both microtubules KIF1C. Two-color directly transported by motors, 3′UTR being sufficient...
The resumption of meiosis in Xenopus arrested oocytes is triggered by progesterone, which leads to polyadenylation and translation Mos mRNA, then activation MAPK pathway. While protein kinase has been reported be essential for re-entry into Xenopus, can undergo germinal vesicle breakdown (GVBD) independently activation, leading us question what the target might if still required. We now demonstrate that indeed necessary, although independent cascade, conversion inactive pre-MPF active MPF....
The c-Mos proto-oncogene product plays an essential role during meiotic divisions in vertebrate eggs. In Xenopus, it is required for progression of oocyte maturation and arrest unfertilized Its degradation after fertilization to early embryogenesis. this study we investigated the mechanisms involved degradation. We present vivo evidence ubiquitin-dependent activated found that not directly dependent on anaphase-promoting factor activator Fizzy/cdc20 but requires cyclin demonstrate B/cdc2...
Cyclin A2 is a key actor in cell cycle regulation. Its degradation mid-mitosis relies on the ubiquitin-proteasome system (UPS). Using high resolution microscopic imaging, we find that cyclin persists beyond metaphase. Indeed, identify novel A2-containing compartment forms dynamic foci. FRET and FLIM analyses show ubiquitylation takes place predominantly these foci before spreading throughout cell. Moreover, inhibition of autophagy proliferating cells induce stabilisation subset, while...
Cellular micro(mi)RNAs are able to recognize viral RNAs through imperfect micro-homologies. Similar the miRNA-mediated repression of cellular translation, this recognition is thought tether RNAi machinery, in particular Argonaute 2 (AGO2) on messengers and eventually modulate virus replication. Here, we unveil another pathway by which AGO2 can interact with retroviral mRNAs. We show that interacts Group Specific Antigen (GAG) core proteins preferentially binds unspliced RNA packaging...
Abstract Cyclin A2 belongs to the core cell cycle regulators and participates in control of both S phase mitosis. However, several observations suggest that it is also endowed with other functions, our recent data shed light on its involvement cytoskeleton dynamic motility. From transcription gene posttranslational modifications, cyclin regulation reveals complexity regulatory network shaping progression. We summarize current knowledge this regulator discuss findings raising possibility...
While first discovered in immunoreceptor signaling, the Syk protein kinase behaves as a tumor and metastasis suppressor epithelial cells. Its reduced expression breast other carcinomas is correlated with decreased survival increased risk, but its action mechanism remains largely unknown. Using phosphoproteomics we found that phosphorylated E-cadherin α-, β-, p120-catenins on multiple tyrosine residues concentrate at intercellular junctions. Increased activation enhanced E-cadherin/catenin...
In pituitary GH3B6 cells, signaling involving the protein kinase C (PKC) multigene family can self-organize into a spatiotemporally coordinated cascade of isoform activation. Indeed, thyrotropin-releasing hormone (TRH) receptor activation sequentially activated green fluorescent (GFP)-tagged or endogenous PKCβ1, PKCα, PKCε, and PKCδ, resulting in their accumulation at entire plasma membrane (PKCβ -δ) selectively cell-cell contacts (PKCα -ε). The duration ranged from 20 s for PKCα to min...