A5027214250- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Hepatitis C virus research
- Advanced Mathematical Modeling in Engineering
- Navier-Stokes equation solutions
- Hepatitis B Virus Studies
- Nuclear Structure and Function
- Oral and Maxillofacial Pathology
- Cancer Diagnosis and Treatment
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- Endodontics and Root Canal Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Computational Fluid Dynamics and Aerodynamics
- Ginseng Biological Effects and Applications
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- MicroRNA in disease regulation
- Natural product bioactivities and synthesis
Institut de Biologie de l'École Normale Supérieure
2018-2023
Université Paris Sciences et Lettres
2020-2023
Centre National de la Recherche Scientifique
2020-2023
Inserm
2020-2023
Pohang University of Science and Technology
2011-2020
Kyung Hee University
2014-2020
École Normale Supérieure - PSL
2018
Eulji University
2007
Eulji University Hospital
2007
Abstract Local translation allows for a spatial control of gene expression. Here, we use high-throughput smFISH to screen centrosomal protein-coding genes, and describe 8 human mRNAs accumulating at centrosomes. These localize different stages during cell cycle with remarkable choreography, indicating finely regulated translational program Interestingly, drug treatments reporter analyses reveal common translation-dependent localization mechanism requiring the nascent protein. Using ASPM...
Abstract Exon junction complexes (EJCs) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division is linked to human neurodevelopmental disorders. In quiescent mNSC immortalized retinal pigment epithelial (RPE1) cells, centrioles form a basal body ciliogenesis. Here, we report that EJCs accumulate at bodies of or RPE1 cells decline when these differentiate resume growth. A high-throughput smFISH screen...
Eukaryotic translation initiation commences at the codon near 5' end of mRNA by a 40S ribosomal subunit, and recruitment ribosome to an is facilitated factors interacting with m(7)G cap and/or poly(A) tail. The recruited then transferred AUG help factors. To understand mechanism which finds codon, we investigated role eIF4G in finding translational codon. An artificial polypeptide fused MS2 was localized downstream reporter gene through MS2-binding sites inserted 3' UTR mRNA. Translation...
c-Src, a non-receptor protein tyrosine kinase, activates NF-κB and STAT3, which in turn triggers the transcription of anti-apoptosis- cell cycle-related genes. c-Src regulates proliferation, motility programmed death. And elevated level activated is related with solid tumor generation. Translation mRNA directed by an IRES element mediates persistent translation under stress conditions when most mRNAs inhibited phosphorylation alpha subunit eIF2 carrying initiator tRNA (tRNAi) to 40S...
Successful viral infection requires intimate communication between virus and host cell, a process that absolutely various proteins. However, current efforts to discover novel proteins as therapeutic targets for are difficult. Here, we developed an integrative-genomics approach predict human genes involved in the early steps of hepatitis C (HCV) infection. By integrating HCV protein associations, co-expression data, tight junction-tetraspanin web specific networks, identified required...
Osteopontin (OPN) is one of important molecular targets in cancer progression, metastasis as a calcium-binding, extracellular-matrix-associated protein the small integrin-binding ligand and, N-linked glycoprotein. In present study, anti-metastatic mechanism ethanol extracts Ocimum sanctum (EEOS) was elucidated on OPN enhanced NCI-H460 non- cell lung cells. Cell viability measured by MTT assay. Adhesion and invasion assays were carried out to see that EEOS inhibited adhesion treated...
Though epigallocatechin-3-gallate (EGCG), a major compound of green tea, has anti-diabetes, anti-obesity, anti-inflammatory, and antitumor effects, the underlying molecular mechanism EGCG was not fully understood so far. Here sensitizing effect to tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL) examined in colorectal cancers. Cotreatment TRAIL synergistically enhanced cytotoxicity sub G1 accumulation, increased number terminal deoxynucleotidyl transferase-dT-mediated dUTP...
MicroRNAs (miRNAs) are small noncoding RNAs that mediate post-transcriptional gene silencing by binding to complementary target mRNAs and recruiting the miRNA-containing ribonucleoprotein complexes mRNAs. However, molecular basis of this is unclear. Here, we show human Ago2 associates with cap-binding protein complex association mediated eIF4GI, a scaffold required for translation initiation. Using cap photo-crosslinking method, closely structure. Taken together, these data suggest eIF4GI...
Though herbal medicines have been used for cancer prevention and treatment, their scientific evidences still remain unclear so far. Thus, complementary alternative medicine (CAM) project has actively executed to reveal the in USA other countries. In present study, we elucidated antitumor mechanism of Chijongdan, an oriental prescription Rhus verniciflua, processed Panax ginseng, Persicaria tinctoria Realgar, that traditionally applied treatment Korea. Chijongdan was prepared with extracts...
Intrahepatic cholangiocarcinoma is a malignant neoplasm arising from the biliary epithelium, which frequently invades adjacent organs or metastasizes to other visceral such as lungs, bones, adrenals, and brain.However, distant skeletal muscle metastasis of has never been described before best our knowledge and, furthermore, Budd-Chiari syndrome secondary intrahepatic also extremely rare.Here we present first case overall c h o l n g i r m p e s t B u d -C syndrome.A 44-year-old man admitted...
Abstract During mitosis, translation of most mRNAs is strongly repressed; none the several explanatory hypotheses suggested can fully explain molecular basis this phenomenon. Here we report that cyclin-dependent CDK11/p58—a serine/threonine kinase abundantly expressed during M phase—represses overall by phosphorylating a subunit (eIF3F) factor eIF3 complex essential for initiation mRNAs. Ectopic expression CDK11/p58 repressed cap-dependent translation, and knockdown nullified translational...
We study the stationary compressible Navier--Stokes equations in a domain containing an interior cut. The cut is internal boundary having vertices with $2\pi$ angle at tip points. It non-Lipschitz boundary. prove existence and piecewise regularity by splitting corner singularity functions tips show that continuity equation solved density function jump discontinuity across curve emanating from rear tip. results vector corresponding to gradient of density. integral through has curvature blows...
Abstract Exon junction complexes (EJC) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division is linked to human neurodevelopmental disorders. In quiescent mNSC immortalized retinal pigment epithelial (RPE1) cells, centrioles form a basal body ciliogenesis. Here, we report that EJCs accumulate at bodies of or RPE1 cells decline when these differentiate resume growth. A high-throughput smFISH screen...
Summary Local translation allows a spatial control of gene expression. Here, we performed dual protein/mRNA localization screen, using smFISH on 523 human cell lines expressing GFP-tagged genes. A total 32 mRNAs displayed specific cytoplasmic localizations, and observed local at unexpected locations, including protrusions, edges, endosomes, Golgi, the nuclear envelope centrosomes, latter being cycle dependent. Quantitation mRNA distribution automatic pattern classification revealed high...
Abstract Local translation allows for a spatial control of gene expression. Here, we used high-throughput smFISH to screen centrosomal protein-coding genes, and describe 8 human mRNAs accumulating at centrosomes. These localize different stages during cell cycle with remarkable choreography, indicating finely regulated translational program Interestingly, drug treatments reporter analyses revealed common translation-dependent localization mechanism requiring the nascent protein. Using ASPM...
Local translation allows a spatial control of gene expression. Here, we performed dual protein/mRNA localization screen, using smFISH on 521 human cell lines expressing GFPtagged genes. A total 32 mRNAs displayed specific cytoplasmic localizations, and observed local at unexpected locations, including protrusions, edges, endosomes, Golgi, the nuclear envelope centrosomes, latter being cell-cycle dependent. Surprisingly, mRNA frequently required ongoing translation, indicating widespread...