A5071773272- Renal and related cancers
- Congenital heart defects research
- Developmental Biology and Gene Regulation
- Urological Disorders and Treatments
- Pluripotent Stem Cells Research
- Genetic and Kidney Cyst Diseases
- Pediatric Urology and Nephrology Studies
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Renal cell carcinoma treatment
- Birth, Development, and Health
- Reproductive Biology and Fertility
- Signaling Pathways in Disease
- Animal Genetics and Reproduction
- Genetics and Neurodevelopmental Disorders
- Pregnancy and preeclampsia studies
- Ovarian cancer diagnosis and treatment
- Neurogenesis and neuroplasticity mechanisms
- Prostate Cancer Treatment and Research
- Macrophage Migration Inhibitory Factor
- Hedgehog Signaling Pathway Studies
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Cytokine Signaling Pathways and Interactions
- dental development and anomalies
McGill University
2012-2024
Université Laval
1993-2023
McGill University Health Centre
2012-2021
William Osler Health System
2014
Research Institute of Molecular Pathology
1997-2008
Vienna Biocenter
2001-2005
In-Q-Tel
2004
Hôtel-Dieu de Québec
2000
Queen Fabiola Children's University Hospital
1988
Hôpital des Enfants
1988
The mammalian kidney develops in three successive steps from the initial pronephros via mesonephros to adult metanephros. Although nephric lineage is specified during induction, no single regulator, including transcription factor Pax2 or Pax8, has yet been identified control this phase of development. In paper, we demonstrate that mouse embryos lacking both and Pax8 are unable form any later structures. these double-mutant embryos, intermediate mesoderm does not undergo...
The mammalian pro- and mesonephros are transient embryonic kidneys essential for urogenital system development. nephric (Wolffian) duct, which is a central constituent of both structures, elongates caudally along stereotypical path to reach the hindlimb level where it induces metanephros (adult kidney) formation, while remaining duct gives rise male genital tract (epidydimis, vas deferens). transcription factors Pax2 Pax8 initiation In cDNA microarray screen genes specifically expressed in...
Pax genes are important regulators of kidney development. In the mouse, homozygous <i>Pax2</i> inactivation results in renal agenesis, a phenotype that has largely precluded analysis <i>Pax</i> gene function during metanephric To address this later function, development was analyzed embryos were compound heterozygous for and <i>Pax8</i>, closely related member family. Both coexpressed differentiating nephrons collecting ducts. At morphological level,...
Metanephric kidney induction critically depends on mesenchymal-epithelial interactions in the caudal region of nephric (or Wolffian) duct. Central to this process, GDNF secreted from metanephric mesenchyme induces ureter budding by activating Ret receptor expressed duct epithelium. A failure regulate pathway is believed be responsible for a large proportion developmental anomalies affecting urogenital system. Here, we show that duct-specific inactivation transcription factor gene Gata3 leads...
Abstract Background Pax2;5;8 transcription factors play diverse roles in vertebrate and invertebrate organogenesis, including the development of inner ear. Past research has suggested various cochlear defects some vestibular Pax2 null mice but details interaction with other Pax family members ear remain unclear. Results We show that Pax2;8 double do not develop an past otocyst stage little to no sensory as well limited transient neuronal development, thus indicating these two are essential...
ABSTRACT Pax2 and Pax5 arose by gene duplication at the onset of vertebrate evolution have since diverged in their developmental expression patterns. They are expressed different organs mouse embryo except for coexpression midbrain-hindbrain boundary (MHB), which functions as an organizing center to control midbrain cerebellum development. During MHB development, is initiated prior transcription, Pax2–/– embryos fail generate posterior cerebellum, whereas Pax5–/– mice exhibit only minor...
Abstract The transcription factor Pax8 is expressed in the developing thyroid gland, inner ear, kidney, and mid‐hindbrain region. mutant mice die only postnatally due to a gland defect. Here we report generation expression analysis of cre allele. Cre recombinase activity was detected known domains cre/+ cre/cre embryos carrying Z/AP transgene, which expresses alkaline phosphatase after Cre‐mediated excision lacZ sequences. Alkaline additionally adrenal facial, vestibulocochlear, cuneate...
Acute lung injury during bacterial infection is associated with neutrophilic inflammation, epithelial cell apoptosis, and disruption of the alveolar-capillary barrier. TLR4 required for in animals exposed to LPS initiates proinflammatory responses part via transcription factor NF-κB. Ligation also a proapoptotic response by activating IFN-β STAT1-dependent genes. We recently demonstrated that mammalian target rapamycin (mTOR), key controller growth survival, can physically interact STAT1...
Urinary tract development depends on a complex series of events in which the ureter moves from its initial branch point nephric duct (ND) to final insertion site cloaca (the primitive bladder and urethra). Defects this maturation process can result malpositioned ureters hydronephrosis, common cause renal disease children. Here, we report that ND into is an unrecognized but crucial step required for proper positioning Ret signaling. Analysis mutant mice at birth reveals hydronephrosis...
Owing to technical advances in single-cell biology, the appreciation of cellular heterogeneity has increased, which aided our understanding organ function, homeostasis, and disease progression. The oviduct (also known as fallopian tube) is distalmost portion female reproductive tract. It essential for reproduction proposed origin high-grade serous ovarian carcinoma (HGSOC). In mammals, morphologically segmented along ovary-uterus axis into four evolutionally conserved regions. unclear,...
Congenital anomalies affecting the ureter-bladder junction are frequent in newborns and often associated with other developmental defects. However, molecular morphological processes underlying these malformations still poorly defined. In this study, we identified leukocyte antigen-related (LAR) family protein tyrosine phosphatase, receptor type, S F (Ptprs Ptprf [also known as Lar], respectively), crucially important for distal ureter maturation craniofacial morphogenesis mouse. Embryos...
β-Catenin/Wnt signaling is essential during early inductive stages of kidney development, but its role postinductive nephron development and maturation not well understood. In this study, we used Pax8Cre mice to target β-catenin deficiency renal epithelial cells at the late S-shaped body stage developing collecting ducts. The conditional knockout formed abnormal kidneys had reduced function. were hypoplastic with a thin cortex; superficial layer tubules was missing. A high proportion...
Despite the high occurrence of congenital abnormalities lower urinary tract in humans, molecular, cellular and morphological aspects their development are still poorly understood. Here, we use a conditional knockout approach to inactivate within nephric duct (ND) lineage two effectors Hippo pathway, Yap Taz. Deletion leads hydronephrotic kidneys with blind-ending megaureters at birth. In mutants, ND successfully migrates towards, contacts, cloaca. However, close analysis reveals that tip...
The kidney and upper urinary tract develop through reciprocal interactions between the ureteric bud surrounding mesenchyme. Ureteric branching forms arborized collecting duct system of kidney, while tips promote nephron formation from dedicated progenitor cells. While cells are relatively well characterized, origin progenitors has received little attention so far. It is established that induced nephric duct, an epithelial derived intermediate mesoderm embryo. However, cell state transitions...
ABSTRACT The primary cilium decorates most eukaryotic cells and regulates tissue morphogenesis maintenance. Structural or functional defects of result in ciliopathies, congenital human disorders affecting multiple organs. Pathogenic variants the ciliogenesis planar cell polarity effectors (CPLANE) genes FUZZY, INTU WDPCP disturb ciliogenesis, causing severe ciliopathies humans mice. Here, we show that loss Fuzzy mice results cilia, accompanied by increased RhoA activity excessive actin...