A5044035281- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Microbial Inactivation Methods
- Ovarian cancer diagnosis and treatment
- Renal and related cancers
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Virus Research Studies
- Animal Genetics and Reproduction
- Tissue Engineering and Regenerative Medicine
- Reproductive System and Pregnancy
- Genetic and Kidney Cyst Diseases
- Biochemical and biochemical processes
- Cancer Cells and Metastasis
- Genetics, Aging, and Longevity in Model Organisms
- Sperm and Testicular Function
- Kruppel-like factors research
- Virus-based gene therapy research
- RNA regulation and disease
- Reproductive Physiology in Livestock
- RNA Interference and Gene Delivery
- Phytochemicals and Antioxidant Activities
- Essential Oils and Antimicrobial Activity
- Assisted Reproductive Technology and Twin Pregnancy
McGill University
2020-2023
Centre For Human Genetics
2021-2023
McGill University Health Centre
2020-2021
Occupational Cancer Research Centre
2021
Owing to technical advances in single-cell biology, the appreciation of cellular heterogeneity has increased, which aided our understanding organ function, homeostasis, and disease progression. The oviduct (also known as fallopian tube) is distalmost portion female reproductive tract. It essential for reproduction proposed origin high-grade serous ovarian carcinoma (HGSOC). In mammals, morphologically segmented along ovary-uterus axis into four evolutionally conserved regions. unclear,...
Abstract The oviduct/fallopian tube is a tube-like structure that extends from the uterus to ovary. It an essential reproductive organ provides environment for internal fertilization and preimplantation development. However, our knowledge of its regional cellular heterogeneity still limited. Here, we examined anatomical complexity mouse oviducts using modern imaging techniques fluorescence reporter lines. We found there are consistent coiling patterns turning points in coiled oviduct serve...
The integrated stress response (ISR) is critical for cell survival under stress. In to diverse environmental cues, eIF2α becomes phosphorylated, engendering a dramatic change in mRNA translation. activation of ISR plays pivotal role the early embryogenesis, but eIF2-dependent translational landscape pluripotent embryonic stem cells (ESCs) largely unexplored. We employ multi-omics approach consisting ribosome profiling, proteomics, and metabolomics wild-type (eIF2α
Abstract Ovarian cancer is the most lethal gynecologic to date. High-grade serous ovarian carcinoma (HGSOC) accounts for cases, and it frequently diagnosed at advanced stages. Here, we developed a novel strategy generate somatic mouse models using combination of in vivo electroporation CRISPR-Cas9–mediated genome editing. Mutation tumor suppressor genes associated with HGSOC two different combinations (Brca1, Tp53, Pten without Lkb1) resulted successfully generation HGSOC, albeit latencies...
The epithelium lining the oviduct or fallopian tube consists of multiciliated and secretory cells, which support fertilization preimplantation development, however, its homeostasis remains poorly understood. CD133/Prom1 expression has been used as a marker to identify adult stem cell populations in various organs often associated with cancer cells that have stem-like properties. Using an antibody targeted CD133 Cre recombinase-based lineage tracing strategy, we found is not stem/progenitor...
Epithelial cells lining the oviduct/fallopian tube are essential in reproduction and have been identified as cell-of-origin high-grade serous ovarian carcinoma (HGSOC). This protocol describes generation of organoids from mouse oviduct epithelial cells, providing a powerful vitro tool to study homeostasis malignant transformation. We also outline for whole-mount immunofluorescence 3D confocal imaging. In addition, we describe approaches viral transduction investigate gene function organoid...
Summary Owing to technical advances in single cell biology, the appreciation of cellular heterogeneity has increased, which aided our understanding organ function, homeostasis and disease progression. The oviduct (also known as fallopian tube humans) is distal-most portion female reproductive tract. It essential for reproduction proposed origin high grade serous ovarian carcinoma (HGSOC). In mammals, morphologically segmented along ovary-uterus axis into four evolutionally conserved regions....
The female reproductive organs present with the earliest aging characteristics, such as a decline in fertility and estrous cyclicity. While age-related changes ovary are well-documented, it is unclear if any age-associated occur other organs, oviduct/fallopian tube. recent recognition of distal end fallopian tube tissue origin high-grade serous tubal ovarian carcinomas (HGSCs), that its patient demographic strongly biased to postmenopausal women, motivated us investigate this organ. At aged...
ABSTRACT The oviduct/fallopian tube is a tube-like structure that extends from the uterus to ovary. It an essential reproductive tissue provides environment for internal fertilization and preimplantation development. However, our knowledge of its regional cellular heterogeneity still limited. Here, we examined anatomical complexity mouse oviducts using modern imaging techniques fluorescence reporter lines. We found there are basic coiling patterns turning points in coiled oviduct can serve...
Owing to technical advances in single cell biology, the appreciation of cellular heterogeneity has increased, which aided our understanding organ function, homeostasis and disease progression. The oviduct (also known as fallopian tube humans) is distal-most portion female reproductive tract. It essential for reproduction proposed origin high grade serous ovarian carcinoma (HGSOC). In mammals, morphologically segmented along ovary-uterus axis into four evolutionally conserved regions. unknown...
The oviduct, called the fallopian tube in humans, is a that extends from uterus and connects to ovary. oviduct an essential organ for fertilization preimplantation embryo development. It consists of five anatomically distinct regions namely infundibulum (INF), ampulla (AMP), ampulla–isthmus junction, isthmus (ISM), uterotubal junction. Each region has mucosal fold patterns unique pair multi-ciliated cells (MCCs) secretory cells, suggesting functions [1, 2]. plays several important roles...
<div>Abstract<p>Ovarian cancer is the most lethal gynecologic to date. High-grade serous ovarian carcinoma (HGSOC) accounts for cases, and it frequently diagnosed at advanced stages. Here, we developed a novel strategy generate somatic mouse models using combination of <i>in vivo</i> electroporation CRISPR-Cas9–mediated genome editing. Mutation tumor suppressor genes associated with HGSOC in two different combinations (<i>Brca1, Tp53, Pten</i> without...
<div>Abstract<p>Ovarian cancer is the most lethal gynecologic to date. High-grade serous ovarian carcinoma (HGSOC) accounts for cases, and it frequently diagnosed at advanced stages. Here, we developed a novel strategy generate somatic mouse models using combination of <i>in vivo</i> electroporation CRISPR-Cas9–mediated genome editing. Mutation tumor suppressor genes associated with HGSOC in two different combinations (<i>Brca1, Tp53, Pten</i> without...
<p>Supplementary Table2</p>
<p>Supplementary Figure1</p>
<p>Summary of tumour genotyping</p>
<p>Supplementary Table2</p>
<p>Supplementary Table4</p>
<p>Summary of tumour genotyping</p>
<p>Supplementary Table3</p>
<p>Supplementary Table</p>
<p>Supplementary Figure1</p>
<p>Supplementary Table</p>
<p>Supplementary Table4</p>