A5045290622- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Image Processing Techniques and Applications
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- Optical Imaging and Spectroscopy Techniques
- Metabolism, Diabetes, and Cancer
- Cardiac electrophysiology and arrhythmias
- Advanced Electron Microscopy Techniques and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Cardiovascular Effects of Exercise
- Electrowetting and Microfluidic Technologies
- Microfluidic and Bio-sensing Technologies
- Medical Imaging Techniques and Applications
- Calcium signaling and nucleotide metabolism
- Cancer Cells and Metastasis
- Advanced X-ray Imaging Techniques
- Lipid Membrane Structure and Behavior
- Advanced Optical Imaging Technologies
- Data Visualization and Analytics
- Biosensors and Analytical Detection
- Cardiac Arrhythmias and Treatments
- Electron and X-Ray Spectroscopy Techniques
- Advanced Biosensing Techniques and Applications
University of Toronto
2017-2025
The University of Sydney
2025
Donnelly College
2024
Institute for Biomedical Engineering
2024
York University
2019-2020
Soochow University
2012
Salmonella utilizes a type 3 secretion system to translocate virulence proteins (effectors) into host cells during infection
Although microscopes and image-analysis software for electron cryomicroscopy (cryo-EM) have improved dramatically in recent years, specimen-preparation methods lagged behind. Most strategies still rely on blotting microscope grids with paper to produce a thin film of solution suitable vitrification. This approach loses more than 99.9% the applied sample requires several seconds, leading problematic air-water interface interactions macromolecules resulting complicating time-resolved studies....
Fouling at liquid-solid interfaces is a pernicious problem for wide range of applications, including those that are implemented by digital microfluidics (DMF). There several strategies have been used to combat surface fouling in DMF, the most common being inclusion amphiphilic surfactant additives droplets be manipulated. Initial studies relied on Pluronic additives, and more recently, Tetronic used, which has allowed manipulation complex samples like serum whole blood. Here, we report our...
The ability to sense and respond osmotic fluctuations is critical for the maintenance of cellular integrity. We used gene co-essentiality analysis identify an unappreciated relationship between TSC22D2, WNK1, NRBP1 in regulating cell volume homeostasis. All these genes have paralogs are functionally buffered osmo-sensing control. Within seconds hyperosmotic stress, TSC22D, WNK, NRBP family members physically associate into biomolecular condensates, a process that dependent on intrinsically...
NADPH/NADP+ redox state supports numerous reactions related to cell growth and survival; yet the full impact is difficult appreciate due organelle compartmentalization of NADPH NADP+. To study glucose-stimulated production in pancreatic beta-cell organelles, we targeted Apollo-NADP+ sensor by first selecting most pH-stable version single-color sensor. We subsequently mTurquoise2-Apollo-NADP+ various organelles confirmed activity cytoplasm, mitochondrial matrix, nucleus, peroxisome. Finally,...
Septin5 interacts with SNARE proteins to regulate exocytosis in neurons, but its role pancreatic β-cells is unknown. Here, we report that abundant rodent and human β-cells, deletion of which dramatically enhances biphasic glucose-stimulated insulin secretion, including type 2 diabetes (T2D). Super-resolution imaging shows preferentially assembled microtubule-plasma membrane contact sites a microtubule-dependent manner, provides discrete harbor for secretory granule anchoring. By decreasing...
Selenium (Se) is linked to physiological homeostasis. Male mice (n = 8/group) were fed control (AIN93G) or diets enriched in sodium selenite (NaSe, 5.6 ppm), methylselenocysteine (Met, 4.7 diphenyl diselenide (DPDS, 14.2 nanoselenium (NanoSe, 2.7 ppm); dietary Se ascertained by inductively-coupled plasma mass spectrometry. At 4 weeks testes, sperm, thyroids, blood and stool collected assess histoarchitecture, circulating hormones (thyroxine, T4; triiodothyronine, T3; thyroid stimulating...
Abstract The intercalated disc (ICD) is a unique membrane structure that indispensable to normal heart function, yet its structural organization not completely understood. Previously, we showed the ICD-bound transmembrane protein 65 (Tmem65) was required for connexin43 (Cx43) localization and function in cultured mouse neonatal cardiomyocytes. Here, investigate functional cellular effects of Tmem65 reductions on myocardium model by injecting CD1 pups (3–7 days after birth) with recombinant...
Abstract Precision-cut-tissues (PCTs), which preserve many aspects of a tissue’s microenvironment, are typically imaged using conventional sample dishes and chambers. These can require large amounts reagent and, when used for flow-through experiments, the shear forces applied on tissues often ill-defined. Their physical design also makes it difficult to image volumes repetitively smaller regions interest in living slice. We report here versatile microfluidic device capable holding mouse or...
Abstract Nematode parasites of humans, livestock and crops dramatically impact human health welfare. Alarmingly, parasitic nematodes animals have rapidly evolved resistance to anthelmintic drugs, traditional nematicides that protect are facing increasing restrictions because poor phylogenetic selectivity. Here, we exploit multiple motor outputs the model nematode C. elegans towards nematicide discovery. This work yielded compounds selectively kill and/or immobilize diverse parasites. We...
Pancreatic islets respond metabolically to glucose by closing KATP channels resulting in Ca2+-influx and insulin secretion. Previous work has revealed the importance of glycolytic flux triggering However, it is unclear whether triggered ('first phase') secretion further amplified Ca2+-stimulation mitochondrial NADH production and/or oxidative phosphorylation (OxPhos). Although commercially available tools have been developed explore islet metabolism, these methods often overlook variability...
Abstract A series of PPV films with different fluorescent emission colors are prepared using a facile approach. Modified from the conventional Wessling route, labile sulfonium groups in polymer precursor (pre‐PPV) partly substituted to various degrees by relatively stable methoxy groups, followed regular thermal elimination step obtain PPV. Different chemical structures among these confirmed IR and photophysical studies. Two film‐preparative processes compared studied SEM confocal...
SUMMARY The ability to sense and respond osmotic fluctuations is critical for the maintenance of cellular integrity. Myriad redundancies have evolved across all facets osmosensing in metazoans, including among water ion transporters, regulators morphology, macromolecular crowding sensors, hampering efforts gain a clear understanding how cells rapid loss. In this study, we harness power gene co-essentiality analysis genome-scale CRISPR-Cas9 screening identify an unappreciated relationship...
Abstract Although microscopes and image analysis software for electron cryomicroscopy (cryo-EM) have improved dramatically in recent years, specimen preparation methods lagged behind. Most strategies still rely on blotting microscope grids with paper to produce a thin film of solution suitable vitrification. This approach loses more than 99.9% the applied sample requires several seconds, leading problematic air-water interface interactions macromolecules resulting complicating time-resolved...
<h2>Abstract</h2> We present the design of a low-cost, portable telecentric digital holographic microscope (P-TDHM) that utilizes off-the-shelf components. describe system's hardware and software elements evaluate its performance by imaging samples ranging from nano-printed targets to live HeLa cells, HEK293 <i>Dolichospermum</i> via both in-line off-axis modes. Our results demonstrate system can acquire high quality quantitative phase images with nanometer axial sub-micron lateral...