A5025134193- Transgenic Plants and Applications
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Toxin Mechanisms and Immunotoxins
- Glycosylation and Glycoproteins Research
- Plant nutrient uptake and metabolism
- Plant responses to elevated CO2
- Plant Stress Responses and Tolerance
- Plant Water Relations and Carbon Dynamics
- Greenhouse Technology and Climate Control
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Autophagy in Disease and Therapy
- Plant Reproductive Biology
- Plant biochemistry and biosynthesis
- Viral Infections and Outbreaks Research
- Lipid metabolism and biosynthesis
- Plant Gene Expression Analysis
- Virus-based gene therapy research
- Seed Germination and Physiology
- Plant-Microbe Interactions and Immunity
- Plant Physiology and Cultivation Studies
- CRISPR and Genetic Engineering
- Studies on Chitinases and Chitosanases
- Plant and fungal interactions
Vienna Biocenter
2018-2024
Vienna BioCenter Core Facilities
2018-2024
BOKU University
2009-2013
Many therapeutic proteins are glycosylated and require terminal sialylation to attain full biological activity. Current manufacturing methods based on mammalian cell culture allow only limited control of this important posttranslational modification, which may lead the generation products with low efficacy. Here we report in vivo protein plants, have been shown be well suited for efficient complex glycoproteins. This was achieved by introduction an entire biosynthetic pathway Nicotiana...
It is well established that proper N-glycosylation significantly influences the efficacy of monoclonal antibodies (mAbs). However, specific immunological relevance individual mAb-associated N-glycan structures currently largely unknown, because heterogeneous profiles mAbs when produced in mammalian cells. Here we report on generation a plant-based expression platform allowing efficient production with homogeneous β1,4-galactosylated structure, major species present serum IgG. This was...
Interactions between the Fc segment of IgG and FcγRs on a variety cells are likely to play an important role in anti-HIV activity Abs. Because nature glycan structure domain is critical determinant Fc-FcγR binding, proper glycosylation may contribute Ab-mediated protection. We have generated five different glycoforms broadly HIV-1-neutralizing mAb 2G12 wild-type glycoengineered plants Chinese hamster ovary cells. Plant-derived exhibited highly homogeneous profiles with single dominant...
Glycoengineering is increasingly being recognized as a powerful tool to generate recombinant glycoproteins with customized N-glycosylation pattern. Here, we demonstrate the modulation of plant glycosylation pathway toward formation human-type bisected and branched complex N-glycans. Glycoengineered Nicotiana benthamiana lacking plant-specific (i.e. β1,2-xylose core α1,3-fucose) was used transiently express human erythropoietin (hEPO) transferrin (hTF) together modified versions...
There is a need for flexible and affordable plant phenotyping solutions basic research breeding. We demonstrate our open source imaging processing solution ('PhenoBox'/'PhenoPipe') provide construction plans, code documentation to rebuild the system. Use of PhenoBox exemplified by studying infection model grass Brachypodium distachyon head smut fungus Ustilago bromivora, comparing phenotypic responses maize with solopathogenic maydis (corn smut) strain effector deletion strains, salt stress...
Acclimation and adaptation of metabolism to a changing environment are key processes for plant survival reproductive success. In the present study, 241 natural accessions Arabidopsis (Arabidopsis thaliana) were grown under two different temperature regimes, 16 °C 6 °C, growth parameters recorded, together with metabolite profiles, investigate genome × effects on metabolome variation. The plasticity metabolism, which was captured by metabolic distance measures, varied considerably between...
Recombinant human erythropoietin (rhEPO), a glycohormone, is one of the leading biopharmaceutical products. The production rhEPO currently restricted to mammalian cell expression systems because rhEPO's highly complex glycosylation pattern, which major determinant for drug-efficacy. Here we evaluate ability plants produce different glycoforms rhEPO. cDNA constructs were delivered Nicotiana benthamiana (N. benthamiana) and transiently expressed by viral based system. Expression levels up 85...
Background Engineered arenavirus vectors have recently been developed to leverage the body’s immune system in fight against chronic viral infections and cancer. Vectors based on Pichinde virus (artPICV) lymphocytic choriomeningitis (artLCMV) encoding a non-oncogenic fusion protein of human papillomavirus (HPV)16 E6 E7 are currently being tested patients with HPV16+ cancer, showing favorable safety tolerability profile unprecedented expansion tumor-specific CD8 + T cells. Although strong...
We investigated early vegetative growth of natural
Recent studies have demonstrated that IgG-Fc fragments (Fcabs) can be engineered to form antigen-binding sites with antibody properties. Thus they may serve as an attractive alternative conventional antibodies in therapeutic applications. The critical influence of Fc glycosylation on effector functions IgGs is well documented; however, whether this applies Fcabs not known. Here we used human cells, wild type, and glycoengineered plants generate four different glycoforms H10-03-6, Fcab...
In all eukaryotes N-glycosylation is the most prevalent protein modification of secretory and membrane proteins. Although capacity individual steps N-glycan processing pathway have been well studied in model plant Arabidopsis thaliana, little attention has paid to characterization glycosylation status We report here structural analysis N-glycans present on endogenous thioglucoside glucohydrolases (myrosinases) TGG1 TGG2 from A. thaliana. All nine sites four are occupied by oligomannosidic...
Abstract In the present study, 241 natural accessions of Arabidopsis thaliana were grown under two different temperature regimes, 16 °C and 6 °C, growth parameters recorded together with metabolite profiles to investigate variation in metabolic responses rates. Primary metabolism rates significantly differed between both conditions. Relative showed high correlations specific pools. Metabolic distances based on whole built from principal component centroids setups. Genomic prediction using...
Roots are the hidden parts of plants, anchoring their above-ground counterparts in soil. They responsible for water and nutrient uptake interacting with biotic abiotic factors The root system architecture (RSA) its plasticity crucial resource acquisition consequently correlate plant performance while being highly dependent on surrounding environment, such as soil properties therefore environmental conditions. Thus, especially crop plants regarding agricultural challenges, it is essential to...
Abstract RNA viruses co-opt the host endomembrane system and organelles to build replication complexes for infection. How responds these membrane perturbations is poorly understood. Here, we explore autophagic response of Arabidopsis thaliana three that hijack different cellular compartments. Autophagy significantly induced within systemically infected tissues, its disruption rendering plants highly sensitive Contrary being an antiviral defense mechanism as previously suggested, quantitative...
ABSTRACT Mobile microRNAs (miRNAs) serve as local and long-distance signals in developmental patterning stress responses plants. However, mechanisms governing the non-cell autonomous activities of miRNAs remain elusive. Here, we show that mutations disrupt microtubule dynamics are specifically defective for actions mobile miRNAs, including miR165/6 is produced endodermis moves to vasculature pattern xylem cell fates Arabidopsis roots. We KTN1, a subunit microtubule-severing enzyme, required...
Abstract Somatic cells acclimate to changes in the environment by temporary reprogramming. Much has been learned about transcription factors that induce these cell-state switches both plants and animals, but how rapidly modulate their proteome remains elusive. Here, we show rapid induction of autophagy during reprogramming triggered phytohormones, immune danger signals. Quantitative proteomics following sequential revealed is required for timely decay previous cellular states tweaking new...
We investigated early vegetative growth of natural Arabidopsis thaliana accessions in cold, non-freezing temperatures, similar to temperatures these plants naturally encounter fall at northern latitudes. found that from latitudes produced larger seedlings than southern latitudes, partly as a result seed size. However, their subsequent when exposed colder was slower. The difference too large be explained by random population differentiation, and is thus suggestive local adaptation, notion...
Abstract Roots are the hidden parts of plants, anchoring their above ground counterparts in soil. They responsible for water and nutrient uptake, as well interacting with biotic abiotic factors The root system architecture (RSA) its plasticity crucial resource acquisition consequently correlate plant performance, while being highly dependent on surrounding environment, such soil properties therefore environmental conditions. Thus, especially crop plants regarding agricultural challenges, it...