A5005535409- Glycosylation and Glycoproteins Research
- Cancer Treatment and Pharmacology
- Breast Cancer Treatment Studies
- Polysaccharides and Plant Cell Walls
- Lymphoma Diagnosis and Treatment
- Advanced Breast Cancer Therapies
- Ovarian cancer diagnosis and treatment
- Lung Cancer Treatments and Mutations
- HER2/EGFR in Cancer Research
- Carbohydrate Chemistry and Synthesis
- Estrogen and related hormone effects
- Food Allergy and Anaphylaxis Research
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Gut microbiota and health
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Bone health and treatments
- Allergic Rhinitis and Sensitization
- Neutropenia and Cancer Infections
- Galectins and Cancer Biology
- Helicobacter pylori-related gastroenterology studies
- Chronic Lymphocytic Leukemia Research
- Probiotics and Fermented Foods
- Transgenic Plants and Applications
Université de Lille
2014-2025
Centre National de la Recherche Scientifique
1994-2024
Unité de Glycobiologie Structurale et Fonctionnelle
2014-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020
Université Lille Nord de France
2014-2016
Canadian Nautical Research Society
2014
BOKU University
2005-2013
Imperial College London
2010
Singleton Hospital
2001-2005
Laboratoire de Chimie
2005
Many therapeutic proteins are glycosylated and require terminal sialylation to attain full biological activity. Current manufacturing methods based on mammalian cell culture allow only limited control of this important posttranslational modification, which may lead the generation products with low efficacy. Here we report in vivo protein plants, have been shown be well suited for efficient complex glycoproteins. This was achieved by introduction an entire biosynthetic pathway Nicotiana...
Two monoclonal antibodies that define distinct plasma cell-associated antigens, termed PCA-1 and PCA-2, were developed against human cell leukemia cells. These antigens are strongly expressed on myelomas, leukemia, plasmacytoma tumor cells, but not detected other lymphoid malignancies of B, T, null, or myeloid origin. PCA-2 either normal T B lymphocytes, weakly granulocytes monocytes. When pokeweed mitogen is used to induce lymphocyte differentiation, when determinants lost plasmacytoid...
In plants, the only known outer-chain elongation of complex N-glycans is formation Lewis a [Fuc alpha1-4(Gal beta1-3)GlcNAc-R] structures. This process involves sequential attachment beta1,3-galactose and alpha1,4-fucose residues by beta1,3-galactosyltransferase alpha1,4-fucosyltransferase. However, exact mechanism underlying epitopes in plants poorly understood, largely because one involved enzymes, beta1,3-galactosyltransferase, has not yet been identified characterized. Here, we report...
We examined the analysis of nucleotides and nucleotide sugars by chromatography on porous graphitic carbon with mass spectrometric detection, a method that evades contamination MS instrument ion pairing reagent. At first, adenosine triphosphate (ATP) other exhibited very poor chromatographic behavior new columns could hardly be eluted from previously cleaned trifluoroacetic acid. Satisfactory performance both older could, however, achieved treatment reducing agent and, unexpectedly,...
UDP-glucose dehydrogenase (UGD) plays a key role in the nucleotide sugar biosynthetic pathway, as its product UDP-glucuronic acid is common precursor for arabinose, xylose, galacturonic acid, and apiose residues found cell wall. In this study we characterize an Arabidopsis thaliana double mutant ugd2,3 that lacks two of four UGD isoforms. This was obtained from cross ugd2 ugd3 single mutants, which do not show phenotypical differences compared with WT. contrast, has strong dwarfed phenotype...
Most processed, e.g. fucosylated, N-glycans on insect glycoproteins terminate in mannose, yet the relevant modifying enzymes require prior action of N-acetylglucosaminyltransferase I. This led to hypothesis that a hexosaminidase acts during course N-glycan maturation. To determine whether Drosophila melanogaster genome indeed encodes such an enzyme, cDNA corresponding fused lobes (fdl), putative beta-N-acetylglucosaminidase with potential transmembrane domain, was cloned. When expressed...
PURPOSE: Tamoxifen is the most commonly prescribed adjuvant therapy for women with breast cancer. It has agonist activity on endometrium and associated an increased risk of endometrial The aim this study was to evaluate whether screening transvaginal ultrasound (TV USS) or without hysteroscopy worthwhile. PATIENTS AND METHODS: A total 487 cancer, 357 treated tamoxifen 130 controls, were screened TV USS, thickness measured. Women thickened underwent outpatient hysteroscopy. RESULTS: Length...
PURPOSE: To compare 6% miltefosine solution (Miltex; Asta Medica, Frankfurt, Germany), a new topical cytostatic drug, with placebo as palliative treatment for cutaneous metastases from breast cancer. PATIENTS AND METHODS: In double-blind, placebo-controlled, multicenter phase III study, total of 52 patients inoperable progressive skin lesions histologically or cytologically confirmed cancer, not manageable by radiotherapy systemic treatment, superficial flat (estimated depth invasion ≤ 1 cm)...
Art v 1, the major allergen of mugwort (Artemisia vulgaris) pollen contains galactose and arabinose. As sera some allergic patients react with natural but not recombinant 1 produced in bacteria, glycosylation may play a role IgE binding human reactions. Chemical enzymatic degradation, mass spectrometry, 800 MHz (1)H (13)C nuclear magnetic resonance spectroscopy indicated proline-rich domain to be glycosylated two ways. We found large hydroxyproline-linked arabinogalactan composed short...
Art v 1, the major pollen allergen of composite plant mugwort (Artemisia vulgaris) has been identified recently as a thionin-like protein with bulky arabinogalactan-protein moiety. A close relative mugwort, ragweed (Ambrosia artemisiifolia) is an important source in North America, and, since 1990, become growing health concern Europe well. Weed pollen-sensitized patients demonstrated IgE reactivity to apparently 29–31 kDa. This reaction could be inhibited by 1. The purified consisted...
Adhesion of Helicobacter pylori to the gastric mucosa is a necessary prerequisite for pathogenesis H. pylori–related diseases. In this study, we investigated GalNAcβ1-4GlcNAc motif (also known as N,N'-diacetyllactosediamine [lacdiNAc]) carried by MUC5AC mucins target bacterial binding human mucosa. The expression LacdiNAc was correlated with localization, and all strains tested adhered significantly motif. Proteomic analysis mutant construction allowed identification yet uncharacterized...
A paradigm regarding rhamnogalacturonans II (RGII) is their strictly conserved structure within a given plant. We developed and employed fast structural characterization method based on chromatography mass spectrometry, allowing analysis of RGII side chains from microgram amounts cell wall. found that structures are much more diverse than so far described. In chain wild-type plants, up to 45% the l-fucose substituted by l-galactose, state seemingly uncorrelated with dimerization capacity....
Hyaluronidase (E.C. 3.2.1.35), one of the three major allergens yellow jacket venom, is a glycoprotein 45 kDa that largely responsible for cross-reactivity wasp and bee venoms with sera allergic patients. The asparagine-linked carbohydrate often appears to constitute common IgE-binding determinant. Using combination MALDI MS HPLC 2-aminopyridine-labelled glycans, we found core-difucosylated paucimannosidic glycans be species in 43-45 band Vespula vulgaris also corresponding bands from five...
A retrospective analysis of 144 patients with stage I-II non-Hodgkin's lymphoma (NHL) treated between June 1968 and December 1980 was performed. Patients were staged by bone marrow biopsy, chest radiography, blood chemistries, either bipedal lymphangiography, computerized axial tomography, or surgical exploration the abdomen. subclassified extent disease; minimal disease defined as less than 10 cm involved one two contiguous sites, while exceeding these limitations considered to have...
Previous studies have reported that plants contain negligible amounts of free or protein-bound N-acetylneuraminic acid (Neu5Ac). This is a major disadvantage for the use as biopharmaceutical expression system, since N-glycans with terminal Neu5Ac residues are important biological activities and half-lives recombinant therapeutic glycoproteins in humans. For synthesis Neu5Ac-containing N-glycans, to acquire ability synthesize its nucleotide-activated derivative, cytidine...
One Health seeks to integrate and balance the health of humans, animals, environmental systems. These three spheres are intricately interconnected through microbiomes, which universally present exchange microbes genes, influencing not only human animal but also key environmental, agricultural, biotechnological processes. Preventing emergence pathogens as well monitoring controlling composition microbiomes microbial effectors including virulence factors, toxins, antibiotics, non-ribosomal...
Abstract Staphylococcus aureus is a predominant cause of chronic lung infections. While the airway environment rich in highly sialylated mucins, interaction S. with sialic acid poorly characterized. Using USA300 as well clinical isolates, we demonstrate that quorum-sensing dysfunction, hallmark adaptation, correlates greater ability to consume free acid, providing growth advantage an air-liquid interface model and vivo. Furthermore, RNA-seq experiment reveals triggers transcriptional...
This study was designed to evaluate new bone resorption and tumour markers as possible alternatives serial plain radiographs for the assessment of response treatment. Thirty-seven patients with newly diagnosed metastases from breast cancer, randomized receive oral pamidronate or placebo tablets in addition anticancer treatment within context a multicentre EORTC trial, who were both assessable radiographic had serum urine samples collected more than 1 month studied. The metabolism measured...
The presence of an α4-fucosyltransferase in plants was first deduced from the characterization Lewis-a glycoepitopes some N-glycans. plant gene encoding recently cloned Beta vulgaris. In present paper we provide evidence for A. thaliana by measurement this glycosyltransferase activity a purified microsomal preparation and immunolocalization Lea epitopes on glycans N-linked to glycoproteins located Golgi apparatus cell surface. corresponding AtFT4 (AY026941) characterized. A unique copy found...
Background: Breast cancer patients with four or more positive axillary lymph nodes who are treated conventional adjuvant therapy have a poor prognosis. In uncontrolled studies, high-dose chemotherapy produced much better results than therapy. We compared the benefits of single cycle and in high-risk breast prospective, unblinded, randomized trial. Methods: Between February 23, 1995, June 29, 1999, 605 had were randomly assigned to treatment (307 298 therapy). The regimen was cycles...