A5072956968- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Vibrio bacteria research studies
- Antifungal resistance and susceptibility
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Fecal contamination and water quality
- Wastewater Treatment and Nitrogen Removal
- Metabolomics and Mass Spectrometry Studies
- Antimicrobial Peptides and Activities
- CRISPR and Genetic Engineering
- Aquaculture Nutrition and Growth
- Bacillus and Francisella bacterial research
- Insect Utilization and Effects
- Plant Pathogenic Bacteria Studies
- Bacterial Genetics and Biotechnology
- Cystic Fibrosis Research Advances
Centre National de la Recherche Scientifique
2020-2024
Institut Necker Enfants Malades
2020-2024
Inserm
2020-2024
Université Paris Cité
2022-2024
University of Chinese Academy of Sciences
2016-2018
Institute of Oceanology
2016-2018
Chinese Academy of Sciences
2016-2018
South China Sea Institute Of Oceanology
2016-2018
Antibiotic-resistant Staphylococcus aureus strains constitute a major public health concern worldwide and are responsible for both care- community-associated infections. Here, we establish robust easy-to-implement model of oral S. infection using Drosophila melanogaster larvae that allowed us to follow the fate at whole-organism level as well host immune responses. Our study demonstrates triggers H2O2 production by via Duox enzyme, thereby promoting antimicrobial peptide through activation...
Abstract Staphylococcus aureus is a predominant cause of chronic lung infections. While the airway environment rich in highly sialylated mucins, interaction S. with sialic acid poorly characterized. Using USA300 as well clinical isolates, we demonstrate that quorum-sensing dysfunction, hallmark adaptation, correlates greater ability to consume free acid, providing growth advantage an air-liquid interface model and vivo. Furthermore, RNA-seq experiment reveals triggers transcriptional...
Abstract Bacterial pathogens adapt and replicate within host cells, while cells develop mechanisms to eliminate them. Using a dual proteomic approach, we characterized the intra-macrophage proteome of facultative intracellular pathogen, Francisella novicida . More than 900 proteins were identified in infected macrophages after 10-h infection. Biotin biosynthesis-related upregulated, emphasizing role biotin-associated genes replication. Conversely, encoded by pathogenicity island (FPI)...
Vibrio alginolyticus is a ubiquitous Gram-negative bacterium which normally distributed in the coastal and estuarine environments. It has been suggested to be an opportunistic pathogen both marine animals humans, Here, completed genome sequence of V. ZJ-T was determined by Illumina high-throughput sequencing.
Colanic acid (CA) is a group I extracellular polysaccharide (EPS) that contributes to resistance against adverse environments in many members of the Enterobacteriaceae. In present study, genetic locus EPSC putatively involved CA biosynthesis was identified Vibrio alginolyticus ZJ-51, which undergoes colony morphology variation between translucent/smooth (ZJ-T) and opaque/rugose (ZJ-O). ZJ-T carries 21 ORFs resembles cluster Escherichia coli K-12. The deletion led decreased EPS biofilm...
Abstract Background Staphylococcus aureus dominates the lung microbiota of children with cystic fibrosis (CF) and persistent clones are able to establish chronic infection for years, having a direct deleterious impact on function. However, in this context, exact contribution S. decline respiratory function CF is not elucidated. Methods To investigate disease, we undertook analysis sequential isogenic isolates recovered from 15 young patients. Results Using an air-liquid model, observed...
目的分析创伤弧菌（Vibrio vulnificus）全基因组框架序列，挖掘感兴趣的遗传位点，探索其是否具有CRISPR系统及其特征。 方法在病虾体内分离获得了一株创伤弧菌TF3，通过Illumina Miseq测序得到基因组框架序列。经注释分析发现其基因组中存在一个CRISPR-Cas系统，命名为CRISPR-CasTF3，进一步分析发现CRISPR-CasTF3位于一个基因岛上，将该基因岛命名为MGIVvuTF3。对CRISPR-CasTF3及MGIVvuTF3的特征和来源进行了分析。...
Abstract Antibiotics multi-resistant Staphylococcus aureus strains constitute a major public health concern worldwide and are responsible of both healthcare- community-associated infections. Here we have established robust simple S. oral infection model, using Drosophila melanogaster larva, which allowed to follow fate at the whole organism level as well host immune responses. Fluorescence microscopy Light sheet 3D imaging revealed bacterial clustering posterior midgut that displays neutral...