A5008058725- Genetic Neurodegenerative Diseases
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Epilepsy research and treatment
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Peptides and Activities
- 3D Printing in Biomedical Research
- Molecular Biology Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Cancer Cells and Metastasis
- Ion channel regulation and function
- Adenosine and Purinergic Signaling
- RNA regulation and disease
- Muscle Physiology and Disorders
- Amino Acid Enzymes and Metabolism
- Insect Utilization and Effects
Inserm
2020-2023
Sorbonne Université
2021-2023
Centre de Recherche en Myologie
2021-2023
Institut des Maladies Génétiques Imagine
2023
Centre de Recherche Saint-Antoine
2022
Institut de Myologie
2021-2022
Université Paris Cité
2020-2021
Centre National de la Recherche Scientifique
2020-2021
Structure fédérative de recherche Necker
2020-2021
Hôpital Necker-Enfants Malades
2020
Antibiotic-resistant Staphylococcus aureus strains constitute a major public health concern worldwide and are responsible for both care- community-associated infections. Here, we establish robust easy-to-implement model of oral S. infection using Drosophila melanogaster larvae that allowed us to follow the fate at whole-organism level as well host immune responses. Our study demonstrates triggers H2O2 production by via Duox enzyme, thereby promoting antimicrobial peptide through activation...
Abstract Brain dysfunction in myotonic dystrophy type 1 (DM1), the prototype of toxic RNA disorders, has been mainly attributed to neuronal misprocessing, while little attention given non-neuronal brain cells. Here, using a transgenic mouse model DM1 that expresses mutant various cell types (neurons, astroglia, and oligodendroglia), we demonstrate astrocytes exhibit impaired ramification polarization vivo defects adhesion, spreading, migration. RNA-dependent toxicity phenotypes are also...
Myotonic dystrophy type 1 (DM1) is a neuromuscular disorder caused by non-coding CTG repeat expansion in the DMPK gene. This mutation generates toxic CUG RNA that interferes with processing of target genes multiple tissues. Despite debilitating neurological impairment, pathophysiological cascade molecular and cellular events central nervous system (CNS) has been less extensively characterized than pathogenesis muscle/cardiac dysfunction. Particularly, contribution different cell types to DM1...
An organoid is a three-dimensional multicellular structure that shows realistic micro-anatomy of an organ. This in vitro model mimics the in vivo environment, architecture and multi-lineage differentiation the original organs allows to answer many interesting biological questions. For these reasons, they are widely used in stem cell, regenerative medicine, toxicology, pharmacology, host-microbe interactions research. In order study organoids, microscopy...
Myotonic dystrophy type 1 (DM1) is a severe neuromuscular disease mediated by toxic gain of function mutant RNAs. The neuropsychological manifestations affect multiple domains cognition and behavior, but their etiology remains elusive. Transgenic DMSXL mice carry the DM1 mutation, show behavioral abnormalities, express low levels GLT1, critical regulator glutamate concentration in synaptic cleft. However, impact homeostasis on neurotransmission unknown. We confirmed reduced uptake...
Extracellular glutamate levels are maintained low by efficient transporters, whose dysfunction can cause neuronal hyperexcitability, excitotoxicity, and neurological disease. While many methods estimate uptake in vitro/ex vivo, a limited number of techniques address transport vivo. Here, we used vivo microdialysis two-in-one approach combining reverse dialysis isotopic to measure ability zero-flow (ZF) quantify extracellular levels. The complementarity both is discussed on methodological...
Abstract Antibiotics multi-resistant Staphylococcus aureus strains constitute a major public health concern worldwide and are responsible of both healthcare- community-associated infections. Here we have established robust simple S. oral infection model, using Drosophila melanogaster larva, which allowed to follow fate at the whole organism level as well host immune responses. Fluorescence microscopy Light sheet 3D imaging revealed bacterial clustering posterior midgut that displays neutral...
Abstract Brain dysfunction in myotonic dystrophy type 1 (DM1), the prototype of toxic RNA disorders, has been mainly attributed to neuronal misprocessing, while little attention given non-neuronal brain cells. Using a transgenic mouse model DM1 that expresses mutant various cell types, we demonstrate astrocytes exhibit impaired ramification and polarization vivo defects adhesion, spreading migration. RNA-dependent toxicity phenotypes was also found human transfected glial In line with...