A5055721343- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Antibiotic Resistance in Bacteria
- Orthopedic Infections and Treatments
- Antimicrobial agents and applications
- Mycobacterium research and diagnosis
- Biochemical and Structural Characterization
- thermodynamics and calorimetric analyses
- Tuberculosis Research and Epidemiology
- Antimicrobial Resistance in Staphylococcus
- Antifungal resistance and susceptibility
- Microbial infections and disease research
- Oral microbiology and periodontitis research
- Advanced Fluorescence Microscopy Techniques
- Bone Tissue Engineering Materials
- Fungal Infections and Studies
- Infective Endocarditis Diagnosis and Management
- Infections and bacterial resistance
- Bacterial Identification and Susceptibility Testing
- Graphene and Nanomaterials Applications
- Infectious Diseases and Mycology
- Viral Infections and Outbreaks Research
- Protein Hydrolysis and Bioactive Peptides
- Clostridium difficile and Clostridium perfringens research
University of Pisa
2013-2025
Centre de Nanosciences et de Nanotechnologies
2023
Berlin-Brandenburger Centrum für Regenerative Therapien
2017-2021
Charité - Universitätsmedizin Berlin
2016-2020
Humboldt-Universität zu Berlin
2017-2020
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2019-2020
Freie Universität Berlin
2017-2020
Istituto Nanoscienze
2012-2019
National Enterprise for NanoScience and NanoTechnology
2015-2019
Abstract In contrast to the many reports of successful real-world cases personalized bacteriophage therapy (BT), randomized controlled trials non-personalized products have not produced expected results. Here we present outcomes a retrospective observational analysis first 100 consecutive BT difficult-to-treat infections facilitated by Belgian consortium in 35 hospitals, 29 cities and 12 countries during period from 1 January 2008 30 April 2022. We assessed how often positive clinical...
Summary The chromosome of Mycobacterium tuberculosis encodes five type VII secretion systems (ESX‐1–ESX‐5). While the role ESX‐1 and ESX‐3 in M. has been elucidated, predictions for function ESX‐5 system came from data obtained marinum , where it transports PPE PE_PGRS proteins modulates innate immune responses. To define this study, we have constructed H37Rv knockout/deletion mutants, inactivating eccA 5 eccD rv1794 esxM genes or ppe25‐pe19 region. Whereas Mtb ko displayed no obvious...
Antimicrobial peptides (AMPs) are increasingly being considered as novel agents against biofilms. The development of AMP-based anti-biofilm strategies strongly relies on the design sequences optimized to target specific features sessile bacterial/fungal communities. Although several AMP databases have been created and successfully exploited for design, all these use data collected tested planktonic microorganisms. Here, an open-access, manually curated database AMPs specifically assayed...
Persister cells (PCs) are a subset of dormant, phenotypic variants regular bacteria, highly tolerant to antibiotics. Generation PCs in vivo may account for the recalcitrance most chronic infections antimicrobial treatment and demands identification new agents able target such cells. The present study explored possibility obtain vitro Pseudomonas aeruginosa Staphylococcus aureus at high efficiency through chemical treatment, test their susceptibility structurally different peptides (AMPs) two...
Abstract Mycobacterium tuberculosis (Mtb) strains are classified into different phylogenetic lineages (L), three of which (L2/L3/L4) emerged from a common progenitor after the loss MmpS6/MmpL6-encoding Mtb-specific deletion 1 region (TbD1). These TbD1-deleted “modern” responsible for globally-spread epidemics, whereas TbD1-intact “ancestral” tend to be restricted specific geographical areas, such as South India and East Asia (L1) or Africa (L7). By constructing characterizing panel...
Abstract Background Prosthetic joint infection (PJI) caused by Pseudomonas aeruginosa represents a severe complication in orthopedic surgery. We report the case of patient with chronic PJI from P. successfully treated personalized phage therapy (PT) combination meropenem. Methods A 62-year-old woman was affected right hip prosthesis since 2016 . The Pa53 (I day 10 mL q8h, then 5 q8h via drainage for 2 weeks) association meropenem (2gr q12h iv) after surgical procedure. 2-year clinical follow...
Abstract Antimicrobial resistance poses a growing threat to public health globally. Multidrug resistant Pseudomonas (P.) aeruginosa is detected in many infected wounds and very challenging treat with antibiotics. An alternative antibiotics use bacteriophages, highly specific viruses able kill even bacteria. This work incorporates anti‐ P. Neko phages into monoaxial coaxial electrospun fibers explore their potential for treating wounds. Phages are blended polyvinyl alcohol (PVA) solution...
Antimicrobial peptides (AMPs) are an abundant and wide class of molecules produced by many tissues cell types in a variety mammals, plant animal species. Linear alpha-helical antimicrobial among the most widespread membrane-disruptive AMPs nature, representing particularly successful structural arrangement innate defense. Recently, have received increasing attention as potential therapeutic agents, owing to their broad activity spectrum reduced tendency induce resistance. The introduction...
Abstract We have previously demonstrated that a soluble form of the human NK cell natural cytotoxicity receptor p44, binds to surface M ycobacterium tuberculosis ( MTB ). Herein, we investigated interaction wall components CWC ) with p44 or T oll‐like 2 TLR 2) and role in direct activation cells upon stimulation . By using several purified bacterial an ELISA , was able bind core mycolyl‐arabinogalactan‐peptidoglycan mAGP as well mycolic acids MA arabinogalactan AG ), while bound...
Systemic administration of antibiotics can cause severe side-effects such as liver and kidney toxicity, destruction healthy gut bacteria, well multidrug resistance. Here, we present a bio-orthogonal chemistry-based strategy toward local prodrug concentration activation. The is based on the inverse electron-demand Diels-Alder chemistry between trans-cyclooctene tetrazine involves biomaterial that concentrate activate multiple doses systemic antibiotic therapy prodrugs at site. We demonstrate...
Staphylococcus epidermidis plays a major role in biofilm-related medical device infections. Herein the anti-biofilm activity of human liver-derived antimicrobial peptide hepcidin 20 (hep20) was evaluated against polysaccharide intercellular adhesin (PIA)-positive and PIA-negative clinical isolates S. epidermidis. Hep20 markedly inhibited biofilm formation bacterial cell metabolism PIA-positive strains, but decrease biomass only partially correlated with viable bacteria. Confocal microscope...
Gram-negative (GN) rods cause about 10% periprosthetic joint infection (PJI) and represent an increasing challenge due to emergence of antimicrobial resistance. Escherichia coli Pseudomonas aeruginosa are among the most common GN-PJI ciprofloxacin is first-line antibiotic. Due fluoroquinolone resistance, we evaluated in vitro activity fosfomycin, ciprofloxacin, gentamicin, alone combinations, against E. P. biofilms. Conventional microbiological tests isothermal microcalorimetry were applied...
To determine the antimicrobial activity against streptococcal biofilm in species mostly isolated from implant-associated infections and examine effect of enzyme treatment on different antibiotics.The activities fosfomycin, rifampicin, benzylpenicillin, daptomycin, gentamicin, levofloxacin, proteinase K their combinations planktonic and/or biofilm-embedded standard laboratory strains Streptococcus agalactiae, pyogenes oralis were investigated vitro by methods isothermal microcalorimetry.MIC...
Acinetobacter baumannii is a leading cause of healthcare-associated infections worldwide. Its various intrinsic and acquired mechanisms antibiotic resistance make the therapeutic challenge even more serious. One promising alternative treatments that increasingly highlighted phage therapy, use bacteriophages to treat bacterial infections. Two phages active against nosocomial carbapenem-resistant A. strain 6077/12, vB_AbaM_ISTD, vB_AbaM_NOVI, were isolated from Belgrade wastewaters, purified,...
Sessile bacteria growing on surfaces are more resistant to standard antibiotics than their planktonic counterpart. Due antimicrobial properties, bacteriophages have re-emerged as a promising approach treat bacterial biofilm-associated infections. Here, we evaluated the ability of two commercially available phage formulations, Staphylococcal bacteriophage (containing monophage Sb-1) and PYO (a polyphage), in preventing eradicating an vitro biofilm methicillin-resistant Staphylococcus aureus...
Due to the widespread resistance of bacteria available drugs, discovery new classes antibiotics is urgently needed, and naturally occurring antimicrobial peptides (AMPs) are considered promising candidates for future therapeutic use. Amphibian skin one richest sources such AMPs. In present study we compared in vitro bactericidal activities five AMPs from three different species anurans against multidrug-resistant clinical isolates belonging often involved nosocomial infections...
One Health seeks to integrate and balance the health of humans, animals, environmental systems. These three spheres are intricately interconnected through microbiomes, which universally present exchange microbes genes, influencing not only human animal but also key environmental, agricultural, biotechnological processes. Preventing emergence pathogens as well monitoring controlling composition microbiomes microbial effectors including virulence factors, toxins, antibiotics, non-ribosomal...
Background/Objectives: The ability of bacteria to form biofilms makes them more tolerant traditional antibiotics. Given the lack new antibiotic development, drug repurposing offers a strategy for discovering treatments. Auranofin (AF), gold-based compound indicated treatment rheumatoid arthritis, shows promising antibacterial activity. This study investigates antimicrobial and antibiofilm activity AF its two derivatives in which thiosugar ligand is replaced by acetylcysteine (AF-AcCys) or...