A5031699708- Bacteriophages and microbial interactions
- Burn Injury Management and Outcomes
- Wound Healing and Treatments
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Disaster Response and Management
- Drug-Induced Adverse Reactions
- Microbial infections and disease research
- Bacillus and Francisella bacterial research
- Trauma and Emergency Care Studies
- Pluripotent Stem Cells Research
- Biomedical Ethics and Regulation
- Plant Virus Research Studies
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Genetics, Bioinformatics, and Biomedical Research
- Dialysis and Renal Disease Management
- Oral Health Pathology and Treatment
- Acute Kidney Injury Research
- Rabies epidemiology and control
- Optical Coherence Tomography Applications
- Aluminum toxicity and tolerance in plants and animals
- Cardiac and Coronary Surgery Techniques
- Injury Epidemiology and Prevention
- Evolution and Genetic Dynamics
Grand Charleroi Hospital
2019-2025
Queen Astrid Military Hospital
2010-2024
Cliniques Universitaires Saint-Luc
2012
Directorate-General Joint Research Centre
2009
We describe the small-scale, laboratory-based, production and quality control of a cocktail, consisting exclusively lytic bacteriophages, designed for treatment Pseudomonas aeruginosa Staphylococcus aureus infections in burn wound patients. Based on succesive selection rounds three bacteriophages were retained from an initial pool 82 P. 8 S. specific prevalent strains Burn Centre Queen Astrid Military Hospital Brussels, Belgium. This phages 14/1 (Myoviridae) PNM (Podoviridae) phage ISP was...
At present there are strong indications that Pseudomonas aeruginosa exhibits an epidemic population structure; clinical isolates indistinguishable from environmental isolates, and they do not exhibit a specific (disease) habitat selection. However, some important issues, such as the worldwide emergence of highly transmissible P. clones among cystic fibrosis (CF) patients spread persistence multidrug resistant (MDR) strains in hospital wards with high antibiotic pressure, remain contentious....
Abstract In contrast to the many reports of successful real-world cases personalized bacteriophage therapy (BT), randomized controlled trials non-personalized products have not produced expected results. Here we present outcomes a retrospective observational analysis first 100 consecutive BT difficult-to-treat infections facilitated by Belgian consortium in 35 hospitals, 29 cities and 12 countries during period from 1 January 2008 30 April 2022. We assessed how often positive clinical...
The worldwide emergence of 'superbugs' and a dry antibiotic pipeline threaten modern society with return to the preantibiotic era. Phages – viruses bacteria could help fight antibiotic-resistant bacteria. Phage therapy was first attempted in 1919 by Felix d'Herelle commercially developed 1930s before being replaced antibiotics most western world. current crisis fueled renaissance phage therapy. inherent potential phages as natural biological bacterium controllers can only be put use if...
Bacteriophages could be used along with burn wound care products to enhance antimicrobial pressure during treatment. However, some of the components topical antimicrobials that are traditionally for prevention and treatment infection might affect activity phages. Therefore, it is imperative determine counteraction therapeutic phage preparations by before application in patients. Five phages, representatives two morphological families (Myoviridae Podoviridae) active against 3 common bacterial...
Multidrug resistant Acinetobacter baumannii and its closely related species A. pittii nosocomialis, all members of the calcoaceticus-baumannii (Acb) complex, are a major cause hospital acquired infection. In burn wound center Queen Astrid military in Brussels, 48 patients were colonized or infected with Acb complex over 52-month period. We report molecular epidemiology these organisms, their clinical impact infection control measures taken. A representative set 157 isolates was analyzed...
Since 1987, keratinocytes have been cultured at the Queen Astrid Military Hospital. These used routinely as auto and allografts on more than 1,000 patients, primarily to accelerate healing of burns chronic wounds. Initially method Rheinwald Green was prepare epithelial autografts, starting from skin samples burn patients using animal-derived feeder layers media containing products. More recently we systematically optimised our production system accommodate scientific advances legal changes....
The transplantation of conventional human cell and tissue grafts, such as heart valve replacements skin for severely burnt patients, has saved many lives over the last decades. late eighties saw emergence engineering with focus on development biological substitutes that restore or improve function. In nineties, at height hype, industry incited policymakers to create a European regulatory environment, which would facilitate strong single market engineered products their starting materials...
The excessive and improper use of antibiotics has led to an increasing incidence bacterial resistance. In Europe the yearly number infections caused by multidrug resistant bacteria is more than 400.000, each year resulting in 25.000 attributable deaths. Few new are pipeline pharmaceutical industry. Early 20th century, bacteriophages were described as entities that can control populations. Although bacteriophage therapy was developed practiced former Soviet republics, clinical setting...
Viable donor skin is still considered the gold standard for temporary covering of burns. Since 1985, Brussels military bank supplies cryopreserved viable cadaveric therapeutic use. Unfortunately, can not be sterilised, which increases risk disease transmission. On other hand, every effort should made to ensure that largest possible part donated processed into high-performance grafts. Cryopreserved allografts fail bacterial or fungal screening are reworked 'sterile' non-viable glycerolised...
A European response plan to burn mass casualty incidents has been jointly developed by the Commission and Burn Association. Upon request for assistance an affected country, outlines a mechanism coordinated international assistance, aiming alleviate burden of care in country offer adequate specialized all patients who can benefit from it. To that aim, Assessment Teams are deployed assess triage patients. Their transportation priority recommendations used distribute outnumbering casualties...
Objective: To describe a case of partial nephrogenic diabetes insipidus in burned patient after prolonged delivery low inspired concentrations sevoflurane via an Anesthetic Conserving Device. Data Sources: Clinical observation. Study Selection: Case report. Extraction: Relevant clinical information. Synthesis: A 34-year-old man was admitted with burns covering 52% his total body surface area. Mechanical ventilation provided during sedation continuous infusions sufentanil and midazolam....
In the last years, publications have questioned classical dose of 35 ml/kg, but are those studies strong enough in terms scientific power order to change our practice? We will try settle some recommendations for clinicians. Manipulation dose, porosity, and combinations yielded promising findings. However, conclusive evidence based on randomized trials remains scarce, limiting practical implementation daily practice. From few designed studies, it is safe say that optimization delivered has a...
Human donor skin allografts are suitable and much used temporary biological (burn) wound dressings. They prepare the excised bed for final autografting form an excellent substrate revascularisation formation of granulation tissue. Two preservation methods, glycerol cryopreservation, commonly by tissue banks long-term storage grafts. The burn surgeons Queen Astrid Military Hospital preferentially use partly viable cryopreserved allografts. After mandatory 14-day bacterial mycological culture,...
Cultures of human epithelial cells (keratinocytes) are used as an additional surgical tool to treat critically burnt patients. Initially, the production environment keratinocyte grafts was regulated exclusively by national regulations. In 2004, European Tissues and Cells Directive 2004/23/EC (transposed into Belgian Law) imposed requirements that resulted in increased costs no significant increase quality and/or safety. 2007, Europe published Regulation (EC) No. 1394/2007 on Advanced Therapy...