A5013077995- Mitochondrial Function and Pathology
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Pain Mechanisms and Treatments
- Viral Infections and Vectors
- Bacterial biofilms and quorum sensing
- SARS-CoV-2 and COVID-19 Research
- COVID-19 and Mental Health
- Biomedical Ethics and Regulation
- Neuroscience and Neuropharmacology Research
- Bacillus and Francisella bacterial research
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- Metabolism and Genetic Disorders
- COVID-19 epidemiological studies
- Monoclonal and Polyclonal Antibodies Research
- Spaceflight effects on biology
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Lipid Membrane Structure and Behavior
- Plant Virus Research Studies
- Electromagnetic Fields and Biological Effects
- Anesthesia and Pain Management
- Fire effects on ecosystems
- ATP Synthase and ATPases Research
KU Leuven
1986-2024
Vrije Universiteit Brussel
2002-2015
Queen Astrid Military Hospital
2002-2015
Leibniz Association
2015
University of Sciences and Technology Houari Boumediene
2007
European Society of Anaesthesiology
2005
International Drug Development Institute (Belgium)
2004
European Resuscitation Council
2003
Ghent University
2002
Civil Service
2002
We describe the small-scale, laboratory-based, production and quality control of a cocktail, consisting exclusively lytic bacteriophages, designed for treatment Pseudomonas aeruginosa Staphylococcus aureus infections in burn wound patients. Based on succesive selection rounds three bacteriophages were retained from an initial pool 82 P. 8 S. specific prevalent strains Burn Centre Queen Astrid Military Hospital Brussels, Belgium. This phages 14/1 (Myoviridae) PNM (Podoviridae) phage ISP was...
At present there are strong indications that Pseudomonas aeruginosa exhibits an epidemic population structure; clinical isolates indistinguishable from environmental isolates, and they do not exhibit a specific (disease) habitat selection. However, some important issues, such as the worldwide emergence of highly transmissible P. clones among cystic fibrosis (CF) patients spread persistence multidrug resistant (MDR) strains in hospital wards with high antibiotic pressure, remain contentious....
The worldwide antibiotic crisis has led to a renewed interest in phage therapy. Since time immemorial phages control bacterial populations on Earth. Potent lytic against pathogens can be isolated from the environment or selected collection matter of days. In addition, have capacity rapidly overcome resistances, which will inevitably emerge. To maximally exploit these advantage over conventional drugs such as antibiotics, it is important that sustainable products are not submitted long...
Aerobic energy metabolism in cells involves the transfer of reducing equivalents from organic molecules to oxygen. NADH is important as a carrier these and feedback regulator glycolysis. We report that micromolar quantities double voltage dependence mitochondrial channel, VDAC, critical pathway for flux metabolites between cytoplasm spaces. In presence NADH, opening closing this channel more sensitive changes membrane potential thus presumably better able respond metabolic conditions. This...
To study the epidemiology of Pseudomonas aeruginosa colonization in a 32-bed burn wound center (BWC), 321 clinical and 45 environmental P. isolates were collected by prospective surveillance culture over 1-year period analyzed serotyping, drug susceptibility testing, amplified fragment length polymorphism (AFLP) analysis. Among 441 patients treated at center, 70 (16%) colonized with aeruginosa, including 12 (17%) who on admission 58 (83%) acquired organism during their stay. Of 48 distinct...
Summary The biodiversity of the bacterium Pseudomonas aeruginosa in an aquatic environment (the Woluwe River, Brussels, Belgium) was analysed. Surface water sampled bimonthly over a 1‐year period (2000–2001) at seven sites evenly dispersed river. Total bacterial counts were performed and P. strains isolated on selective medium. A weighed out sample 100 randomly chosen presumptive isolates further set data consisting nucleotide sequence oprL gene, DNA‐based fingerprint ( mplified f ragment l...
Mitochondria with intact outer membrane (99% based on cytochrome c impermeability) were isolated and used to measure the permeability of their ADP. beta-NADH reduced in a concentration-dependent manner (KD = 87 +/- 5 microM) by factor 6. alpha-NADH beta-NAD+ cannot mimic action beta-NADH. The mitochondrial membranes become rate-limiting presence at low, physiologically relevant, ADP concentrations (
The worldwide emergence of 'superbugs' and a dry antibiotic pipeline threaten modern society with return to the preantibiotic era. Phages – viruses bacteria could help fight antibiotic-resistant bacteria. Phage therapy was first attempted in 1919 by Felix d'Herelle commercially developed 1930s before being replaced antibiotics most western world. current crisis fueled renaissance phage therapy. inherent potential phages as natural biological bacterium controllers can only be put use if...
The seriously and globally increasing bacterial multi-drug resistance calls out
Bacteria can have population structures ranging from the fully sexual to highly clonal. Despite numerous studies, structure of Pseudomonas aeruginosa is still somewhat contentious. We used a polyphasic approach in order shed new light on this issue. A data set consisting three outer membrane (lipo)protein gene sequences (oprI, oprL and oprD), DNA-based fingerprint (amplified fragment length polymorphism), serotype pyoverdine type 73 P. clinical environmental isolates, collected across world,...
Summary Genomes are constantly evolving. Our report highlights the wide mutational diversity of clinical as well environmental isolates, compared with laboratory strain(s), through systematic genetic analysis a chromosomal porin gene ( oprD ) in relation to specific antibiotic resistance. Mutational inactivation is associated carbapenem resistance Pseudomonas aeruginosa . The sequence 55 natural isolates obtained from across world – sources diverse patients and rhizospheres was analysed. A...
The treatment of infectious diseases with antibiotics is becoming increasingly challenging. Very few new antimicrobials are in the pharmaceutical industry pipeline. One potential alternatives for phage therapy. Major obstacles clinical application bacteriophages a false perception viruses as 'enemies life' and lack specific frame therapy current Medicinal Product Regulation. Short-term borderline solutions under responsibility Medical Ethical Committee and/or umbrella Declaration Helsinki...
Research during the pandemic has demonstrated that rapid shift to emergency distance learning impacted students' emotions. What explains this link remains a sparsely explored question. Because many students report negative experiences while video conferencing learning, one avenue yet be is whether attitudes towards may explain between and As such, explore question, total of 558 college 219 parents or guardians K-12 completed survey about their positive emotions learning. Across both samples,...
Concerns with protecting privacy, especially of online data, has been a goal privacy scholarship for years. Because most data are transferred online, many instruments focus on environments. However, when is invaded and mishandled, the consequences, including emotional ramifications, extend beyond space into offline world. Thus, we developed CPIP, measure concern. We were able to (1) determine top four domains informational (2) correlate that concern outcomes showing people high concerns felt...
The COVID-19 pandemic forced workers to pivot working from home (WFH), shifting a dominantly offline online workplace, often conducting this shift work using video conference meetings. Although businesses tried seamlessly continue business conferencing, research on how productivity has changed during the been mixed. To better understand impacted pandemic, despite decades of prior demonstrating effectiveness WFH, we conducted three quantitative and qualitative studies with April 2020 2021....