A5055006427- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Calcium signaling and nucleotide metabolism
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Soybean genetics and cultivation
- Plant Parasitism and Resistance
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Cellular transport and secretion
- Plant responses to water stress
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Fungal and yeast genetics research
- Heat shock proteins research
- thermodynamics and calorimetric analyses
- Biochemical and Molecular Research
- Studies on Chitinases and Chitosanases
- ATP Synthase and ATPases Research
- Polyamine Metabolism and Applications
Gregor Mendel Institute of Molecular Plant Biology
2020-2024
Austrian Academy of Sciences
2020-2024
Vienna Biocenter
2020-2024
Medical University of Vienna
2020-2024
University of Vienna
2023
Research Institute of Molecular Pathology
2020
Eukaryotes have evolved various quality control mechanisms to promote proteostasis in the endoplasmic reticulum (ER). Selective removal of certain ER domains via autophagy (termed as ER-phagy) has emerged a major mechanism. However, degree which ER-phagy is employed by other branches ER-quality remains largely elusive. Here, we identify cytosolic protein, C53, that specifically recruited autophagosomes during ER-stress, both plant and mammalian cells. C53 interacts with ATG8 distinct binding...
UFMylation involves the covalent modification of substrate proteins with UFM1 (Ubiquitin-fold modifier 1) and is important for maintaining ER homeostasis. Stalled translation triggers ER-bound ribosomes activates C53-mediated autophagy to clear toxic polypeptides. C53 contains noncanonical shuffled ATG8-interacting motifs (sAIMs) that are essential ATG8 interaction initiation. However, mechanistic basis sAIM-mediated remains unknown. Here, we show sAIMs conserved across eukaryotes but...
Reticulophagy, the selective autophagy of endoplasmic reticulum (ER) components, is known to operate in eukaryotes from yeast and unicellular algae animals plants. Thus far, only ER-stress induced reticulophagy was reported analyzed In this study we characterize a pathway Arabidopsis thaliana that triggered by dark-induced starvation but not ER stress. This defined previously ATG8-interacting proteins, ATI1 ATI2. We further identified ER-localized MSBP1 (Membrane Steroid Binding Protein 1)...
Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been extensively studied, maturation, i.e., delivery and fusion with vacuole, remains largely unknown in plants. Here, we have identified an autophagy adaptor, CFS1, directly interacts marker ATG8 localizes on both membranes of autophagosome. form normally Arabidopsis thaliana cfs1 mutants, but their is disrupted. CFS1’s...
Plants rely on autophagy and membrane trafficking to tolerate stress, combat infections, maintain cellular homeostasis. However, the molecular interplay between is poorly understood. Using an AI-assisted approach, we identified Rab3GAP-like (Rab3GAPL) as a key node that controls plant negatively. Rab3GAPL suppresses by binding ATG8, core adaptor, deactivating Rab8a, small GTPase essential for autophagosome formation defense-related secretion. reduces autophagic flux in three model species,...
Reticulophagy, the autophagic degradation of endoplasmic reticulum, is crucial to maintain ER homeostasis during stress. Although several reticulophagy receptors have been discovered recently, most them studied using nutrient starvation. How macroautophagy/autophagy cross-talks with other ER-quality control mechanisms largely unknown. Using ATG8-based affinity proteomics in model plant Arabidopsis thaliana, we identified AT5G06830/C53, a soluble protein that directly interacts ATG8....
Abstract UFMylation mediates the covalent modification of substrate proteins with UFM1 (Ubiquitin-fold modifier 1) and regulates selective degradation endoplasmic reticulum (ER) via autophagy (ER-phagy) to maintain ER homeostasis. Specifically, collisions ER-bound ribosomes trigger ribosome UFMylation, which in turn activates C53-mediated that clears toxic incomplete polypeptides. C53 has evolved non-canonical shuffled ATG8 interacting motifs (sAIMs) are essential for interaction initiation....
Abstract RNA viruses co-opt the host endomembrane system and organelles to build replication complexes for infection. How responds these membrane perturbations is poorly understood. Here, we explore autophagic response of Arabidopsis thaliana three that hijack different cellular compartments. Autophagy significantly induced within systemically infected tissues, its disruption rendering plants highly sensitive Contrary being an antiviral defense mechanism as previously suggested, quantitative...
Abstract Selective autophagy is a fundamental protein quality control pathway that safeguards proteostasis by degrading damaged or surplus cellular components, particularly under stress. This process orchestrated selective receptors (SARs) direct specific cargo for degradation. While significant strides have been made in understanding the molecular framework of autophagy, diversity SAR repertoires across species remain largely unexplored. Through comparative interactome analysis five model...
Abstract Plants rely on autophagy and membrane trafficking to tolerate stress, combat infections, maintain cellular homeostasis. However, the molecular interplay between is poorly understood. Using an AI-assisted approach, we identified Rab3GAP-like (Rab3GAPL) as important node that controls plant negatively. Rab3GAPL suppresses by binding ATG8, core adaptor, deactivating Rab8a, a small GTPase essential for autophagosome formation defense-related secretion. from Nicotiana benthamiana , but...
Plants rely on autophagy and membrane trafficking to tolerate stress, combat infections, maintain cellular homeostasis. However, the molecular interplay between is poorly understood. Using an AI-assisted approach, we identified Rab3GAP-like (Rab3GAPL) as a key node that controls plant negatively. Rab3GAPL suppresses by binding ATG8, core adaptor, deactivating Rab8a, small GTPase essential for autophagosome formation defenserelated secretion. reduces autophagic flux in three model species,...
Summary Eukaryotes have evolved various quality control mechanisms to promote proteostasis in the ER. Selective removal of certain ER domains via autophagy (termed as ER-phagy) has emerged a major mechanism. However, degree which ER-phagy is employed by other branches ER-quality remains largely elusive. Here, we identify cytosolic protein, C53, that specifically recruited autophagosomes during ER-stress, both plant and mammalian cells. C53 interacts with ATG8 distinct binding epitope,...