A5017695319- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Single-cell and spatial transcriptomics
- Geotechnical Engineering and Soil Stabilization
- Educator Training and Historical Pedagogy
- MicroRNA in disease regulation
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Religious Education and Schools
- DNA Repair Mechanisms
Austrian Academy of Sciences
2020-2023
Institute of Molecular Biotechnology
2020-2023
Vienna Biocenter
2020-2023
German Primate Center
2017
Accurate repair of DNA double-strand breaks (DSBs) is essential for genome stability, and defective underlies diseases such as cancer. Homologous recombination uses an intact homologous sequence to faithfully restore damaged DNA, yet how broken ends find sites in a containing billions non-homologous bases remains unclear. Here, we introduce sister-pore-C, high-resolution method mapping intra- trans-molecular interactions replicated chromosomes. We show that DSBs reshape chromosome...
Abstract Despite major advances on miRNA profiling and target predictions, functional readouts for endogenous miRNAs are limited frequently lead to contradicting conclusions. Numerous approaches including high-throughput miRISC complex evaluations suggest that the miRNAome differs from predictions based quantitative sRNA profiling. To resolve apparent contradiction of expression versus function, we generated applied a fluorescence reporter gene assay enabling single cell analysis. This...
Abstract The three-dimensional organization of the genome supports regulated gene expression, recombination, DNA repair, and chromosome segregation during mitosis. Chromosome conformation capture (Hi-C) 1–3 has revealed a complex genomic landscape internal structures in vertebrate cells 4–11 yet how sister chromatids topologically interact replicated chromosomes remained elusive due to their identical sequences. Here, we present sister-chromatid-sensitive Hi-C (scsHi-C) based on nascent...
Abstract Genome browsers facilitate integrated analysis of multiple genomics datasets yet visualize only a few regions at time and lack statistical functions for extracting meaningful information. We present HiCognition, visual exploration machine-learning tool based on new genomic region set concept, enabling detection patterns associations between 3D chromosome conformation collections 1D profiles any type. By revealing how transcription cohesion subunit isoforms contribute to...
Abstract The 3D organization of the genome and epigenetic marks play important roles in gene expression, DNA repair, chromosome segregation. Understanding how structure composition chromatin fiber contribute to function requires integrated analysis multiple genomics datasets from various techniques, experimental conditions, cell states. Genome browsers facilitate such analysis, yet currently visualize only a few regions at time lack statistical functions that are often necessary extract...