A5033269313- RNA modifications and cancer
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Mesenchymal stem cell research
- Bone and Joint Diseases
- Bone Metabolism and Diseases
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
- Advanced biosensing and bioanalysis techniques
- Parvovirus B19 Infection Studies
- Immune cells in cancer
University of Göttingen
2020-2023
Universitätsmedizin Göttingen
2020-2023
German Primate Center
2017-2020
Efficient transduction tools are a hallmark for both research and therapy development. Here, we introduce new insights into the generation of lentiviral vectors with improved performance by utilizing producer cells increased production rates extracellular vesicles through CD9 overexpression. Most human secrete small from their surface (microvesicles) or intraluminal endosome-derived membranes (exosomes). In particular, enhanced levels tetraspanin result in significantly numbers exosome-like...
Modified nucleotides in tRNAs are important determinants of folding, structure and function. Here we identify METTL8 as a mitochondrial matrix protein active RNA methyltransferase responsible for installing m3C32 the human (mt-)tRNAThr mt-tRNASer(UCN). crosslinks to anticodon stem loop (ASL) many mt-tRNAs cells, raising question how methylation target specificity is achieved. Dissection mt-tRNA recognition elements revealed U34G35 t6A37/(ms2)i6A37, present concomitantly only ASLs two...
Research on cell-free vesicles revealed a multitude of characteristics, in particular microvesicles and exosomes, that range from their potential as biomarkers to function horizontal transfer genetic information cell also include supportive functions viral infection. Exosome-associated adeno-associated viruses (exo-AAVs) are interest for the past couple years, because they introduced new source highly potent recombinant AAVs with improved features, including accelerated transduction rates...
Mesenchymal stromal cells (MSC) have been used in over 800 clinical trials with encouraging results the field of transplant medicine and chronic inflammatory diseases. Today, Umbilical Cord (UC)-derived MSC are second leading source for purposes, mainly due to its easy access superior immune-modulatory effects. Although underlying molecular mechanisms immune-suppressive activities not fully understood, research last decade strongly suggests that MSC-mediated benefits closely related...
MicroRNAs (miRNAs) are important regulators of eukaryotic gene expression and their dysfunction is often associated with cancer. Alongside the canonical miRNA biogenesis pathway involving stepwise processing export pri- pre-miRNA transcripts by microprocessor complex, Exportin 5 Dicer, several alternative mechanisms production have been described. Here, we reveal that atypical box C/D snoRNA U3, which functions as a scaffold during early ribosome assembly, source. We show unique stem-loop...
Abstract Despite major advances on miRNA profiling and target predictions, functional readouts for endogenous miRNAs are limited frequently lead to contradicting conclusions. Numerous approaches including high-throughput miRISC complex evaluations suggest that the miRNAome differs from predictions based quantitative sRNA profiling. To resolve apparent contradiction of expression versus function, we generated applied a fluorescence reporter gene assay enabling single cell analysis. This...
Rebalancing of the RANKL/OPG system seems to be an effective treatment strategy in postmenopausal osteoporosis. Here, we evaluate knockdown RANKL by in-vivo-delivered siRNA a rat model Virus-like-particles (VLPs) derived from polyoma JC virus were used for delivering ovariectomized (OVX) rats. 48 rats and treated with either 17β-estradiol (E2), VLPs containing (siRANKL), or non-cognate (siCtrl). All OVX groups subdivided into prophylaxis group (PG) therapy (TG). The PG received directly...
Abstract Regulation of RNA helicase activity, often accomplished by protein cofactors, is essential to ensure target specificity within the complex cellular environment. The largest family cofactors are G-patch proteins, but cognate helicases and functions numerous human proteins remain elusive. Here, we discover that GPATCH4 a stimulatory cofactor DHX15 interacts with DEAH box in nucleolus via residues its domain. We reveal associates pre-ribosomal particles, crosslinks transcribed...
MicroRNA-induced gene regulation is a growing field in basic and translational research. Examining this directly cells necessary to validate high-throughput data originated from RNA sequencing technologies. For several studies employ luciferase-based reporters that usually measure the whole cell population, which comes with low resolution for complexity of miRNA-induced regulation. Here, we provide protocol using dual-fluorescence reporter flow cytometry reaching single resolution; contains...