A5053780425- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Wnt/β-catenin signaling in development and cancer
- Reproductive Biology and Fertility
- Genomics and Phylogenetic Studies
- Prenatal Screening and Diagnostics
- Acute Myeloid Leukemia Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Bacterial Genetics and Biotechnology
- Plant Molecular Biology Research
- Skin and Cellular Biology Research
- Extracellular vesicles in disease
- Cancer Cells and Metastasis
- Mitochondrial Function and Pathology
- Renal and related cancers
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- Photoreceptor and optogenetics research
- Planarian Biology and Electrostimulation
- Nuclear Structure and Function
- Advanced MRI Techniques and Applications
- Lipid Membrane Structure and Behavior
- Microtubule and mitosis dynamics
University College London
2019-2024
University of Pennsylvania
2022-2024
California Institute for Regenerative Medicine
2022-2023
Anna Needs Neuroblastoma Answers
2023
Agency for Science, Technology and Research
2020-2022
Institute of Molecular and Cell Biology
2020-2022
Abstract During preimplantation development, contractile forces generated at the apical cortex segregate cells into inner and outer positions of embryo, establishing cell mass (ICM) trophectoderm. To which extent these influence ICM-trophectoderm fate remains unresolved. Here, we found that nuclear lamina is coupled to via an F-actin meshwork in mouse human embryos. Actomyosin contractility increases during upregulating Lamin-A levels, but upon internalization lose their downregulate...
Abstract Many amniote vertebrate species including humans can form identical twins from a single embryo, but this only occurs rarely. It has been suggested that the primitive-streak-forming embryonic region emits signals inhibit streak formation elsewhere involved, how they are transmitted and act not elucidated. Here we show short tracks of calcium firing activity propagate through extraembryonic tissue via gap junctions prevent ectopic primitive in chick embryos. Cross-regulation an...
Splicing factor mutations are common in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML), but how they alter cellular functions is unclear. We show that the pathogenic SRSF2P95H/+ mutation disrupts splicing of mitochondrial mRNAs, impairs complex I function, robustly increases mitophagy. also identified a surveillance mechanism by which dysfunction modifies mitophagy activator PINK1 to remove poison intron, increasing stability abundance mRNA protein. SRSF2P95H-induced...
Significance Previous studies have suggested that the amniote node (Hensen’s node) contains a small population of self-renewing resident cells whose progeny progressively lay down axial tissues, including notochord and somites. This can only be demonstrated definitively at level single cells. Here we ask whether is an environment confer this behavior on enter it. We challenge in vivo mRNA-profile these to demonstrate indeed do this, thus show acts as instructive niche.
Abstract Background Older age is the main risk factor for chronic lung diseases including idiopathic pulmonary fibrosis (IPF). Halting or reversing progression of IPF remains an unmet clinical need due to limited knowledge underlying mechanisms. The circulatory system, composed blood (pulmonary and bronchial) lymphatic vessels networks, has been implicated in pathophysiology elderly people, based solely on reports altered density increased permeability vessels. Aim We aimed define...
In warm-blooded vertebrate embryos (mammals and birds), the body forms from a growth zone at tail end. Hensen’s node, region which induces patterns neural axis is located within this zone. The node also contains precursors of neural, mesodermal endodermal structures along midline has been suggested to contain small population resident stem cells. However, it unknown whether rest constitutes an instructive cell niche, specifying behaviour. Here we combine transplantation single in vivo with...
Splicing factor mutations are common in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML), but how they alter cellular functions is unclear. We show that the pathogenic
ABSTRACT The proportion of babies born from in vitro fertilization (IVF) is rising at an exponential rate, highlighting the importance that we have a comprehensive understanding human preimplantation development and determinants embryo quality. early divisions after but before implantation resulting mitotic errors been studied primarily mouse embryo; however, thus far lacked technology to characterize these critical steps humans. Establishing approach can bypass genetic manipulation...
Flagella are necessary for bacterial movement and contribute to various aspects of virulence. They complex cylindrical structures built multiple molecular rings with self-assembly properties. The flagellar rotor is composed the MS-ring C-ring. FliG protein C-ring central assembly function due its roles in linking torque transmission from stator rotor. No high-resolution structure an assembled has been resolved date, conformation adopted by within ring unclear variations available...
Abstract Somites are transient structures derived from the pre‐somitic mesoderm (PSM), involving mesenchyme‐to‐epithelial transition (MET) where cells change their shape and polarize. Using Scanning electron microscopy (SEM), immunocytochemistry confocal microscopy, we study progression of these events along tail‐to‐head axis embryo, which mirrors somitogenesis (younger located more caudally). SEM revealed that PSM epithelialization is a gradual process, begins much earlier than previously...
Abstract We present a detailed analysis of gene expression in the 2-day (HH12) embryonic chick heart. RNA-seq 13 micro-dissected regions reveals regionalised 15,570 genes. Of these, 132 were studied by situ hybridisation and subset (38 genes) was mapped Optical Projection Tomography or serial sectioning to build 3-dimensional atlas expression. display this with novel interactive 3-D viewer as stacks sections, revealing boundaries domains overlap. Analysis also defines some sub-regions...