A5008277442- RNA Research and Splicing
- RNA modifications and cancer
- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Prion Diseases and Protein Misfolding
- Nuclear Structure and Function
- Cellular Mechanics and Interactions
- Ubiquitin and proteasome pathways
- Protein Structure and Dynamics
- Amyotrophic Lateral Sclerosis Research
- RNA regulation and disease
- Enzyme Structure and Function
- Genetic Neurodegenerative Diseases
- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Microfluidic and Bio-sensing Technologies
- Biochemical and Molecular Research
- Neurogenetic and Muscular Disorders Research
- Autophagy in Disease and Therapy
- Advanced Proteomics Techniques and Applications
- Proteins in Food Systems
- Neurological diseases and metabolism
- Spaceflight effects on biology
TU Dresden
2019-2025
Center for Systems Biology Dresden
2019-2024
Max Planck Institute of Molecular Cell Biology and Genetics
2015-2024
National Center for Genetic Engineering and Biotechnology
2020-2024
Regione del Veneto
2023
Institute of Molecular Biology
2018
University of Bonn
2001-2014
Max Planck Society
2013
Whitehead Institute for Biomedical Research
2007-2012
Howard Hughes Medical Institute
2007
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular...
RNA and membraneless organelles Membraneless compartments can form in cells through liquidliquid phase separation (see the Perspective by Polymenidou). But what prevents these cellular condensates from randomly fusing together? Using RNA-binding protein (RBP) Whi3, Langdon et al. demonstrated that secondary structure of different components determines distinct biophysical biological properties two types Whi3 forms. Several RBPs, such as FUS TDP43, contain prion-like domains are linked to...
ATP boosts protein solubility Adenosine triphosphate (ATP) has well-characterized roles in providing energy for biochemical reactions within cells. Patel et al. find that may also enhance solubility, which could help explain why such high concentrations of are maintained cells (see the Perspective by Rice and Rosen). Protein can exceed 100 mg/ml. The authors found at act as a hydrotrope to solubilize hydrophobic proteins. results raise possibility influence processes aggregation occur...
Stressed cells shut down translation, release mRNA molecules from polysomes, and form stress granules (SGs) via a network of interactions that involve G3BP. Here we focus on the mechanistic underpinnings SG assembly. We show that, under non-stress conditions, G3BP adopts compact auto-inhibited state stabilized by electrostatic intramolecular between intrinsically disordered acidic tracts positively charged arginine-rich region. Upon unfolded mRNAs outcompete auto-inhibitory interactions,...
Biophysical responses of proteins to stress Much recent work has focused on liquid-liquid phase separation as a cellular response changing physicochemical conditions. Because responds critically small changes in conditions such pH, temperature, or salt, it is principle an ideal way for cell measure and respond the environment. Small pH could, instance, induce compartments that store, protect, inactivate proteins. Franzmann et al. used yeast translation termination factor Sup35 model...
RNA-protein (RNP) granules have been proposed to assemble by forming solid RNA/protein aggregates or through phase separation into a liquid phase. Which model describes RNP in living cells is still unclear. In this study, we analyze P bodies budding yeast and find that they liquid-like properties. Surprisingly, stress adopt different material state, which reminiscent of protein controlled disaggregases. By using an assay ectopically nucleate granules, further establish granule formation does...
In the post-genomic era, academic and biotechnological research is increasingly shifting its attention from single proteins to analysis of complex protein networks. This change in experimental design requires use simple experimentally tractable organisms, such as unicellular eukaryote Saccharomyces cerevisiae, a range new high-throughput techniques. The Gateway system has emerged powerful cloning method that allows for vitro recombination DNA with high speed, accuracy reliability. Two...
Cells can enter into a dormant state when faced with unfavorable conditions. However, how cells and recover from this is still poorly understood. Here, we study dormancy in different eukaryotic organisms find it to be associated significant decrease the mobility of organelles foreign tracer particles. We show that reduced caused by an influx protons marked acidification cytoplasm, which leads widespread macromolecular assembly proteins triggers transition cytoplasm solid-like increased...
Article4 April 2017Open Access Transparent process An aberrant phase transition of stress granules triggered by misfolded protein and prevented chaperone function Daniel Mateju Max Planck Institute Molecular Cell Biology Genetics, Dresden, Germany Search for more papers this author Titus M Franzmann Avinash Patel Andrii Kopach Edgar E Boczek Shovamayee Maharana Hyun O Lee Serena Carra Department Biomedical, Metabolic Neural Sciences, University Modena Reggio Emilia, Modena, Italy Anthony A...
Abstract Liquid–liquid phase separation of proteins underpins the formation membraneless compartments in living cells. Elucidating molecular driving forces underlying protein transitions is therefore a key objective for understanding biological function and malfunction. Here we show that cellular proteins, which form condensates at low salt concentrations, including FUS, TDP-43, Brd4, Sox2, Annexin A11, can reenter phase-separated regime high concentrations. By bringing together experiments...
Non-centrosomal microtubule bundles play important roles in cellular organization and function. Although many diverse proteins are known that can bundle microtubules, biochemical mechanisms by which cells could locally control the nucleation formation of understudied. Here, we demonstrate concentration tubulin into a condensed, liquid-like compartment composed unstructured neuronal protein tau is sufficient to nucleate bundles. We show that, under conditions macro-molecular crowding, forms...
The formation of membrane-less organelles and compartments by protein phase separation is an important way in which cells organize their cytoplasm nucleoplasm. In vitro assays with purified proteins have become the standard to investigate that form compartments. By now, various been tested for ability separate liquid condensates vitro. However, phase-separating are often aggregation-prone difficult purify handle. As a consequence, results from differ between labs not easily reproduced. Thus,...