A5056336708- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- RNA Research and Splicing
- Force Microscopy Techniques and Applications
- 3D Printing in Biomedical Research
- Barrier Structure and Function Studies
- Cellular transport and secretion
- Nanopore and Nanochannel Transport Studies
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Cell Image Analysis Techniques
- Photosynthetic Processes and Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- nanoparticles nucleation surface interactions
- Genomics and Chromatin Dynamics
- Near-Field Optical Microscopy
- S100 Proteins and Annexins
- Spectroscopy and Quantum Chemical Studies
- Hippo pathway signaling and YAP/TAZ
- Cancer Cells and Metastasis
- Sphingolipid Metabolism and Signaling
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Cell Adhesion Molecules Research
Max Planck Institute of Molecular Cell Biology and Genetics
2016-2025
Center for Systems Biology Dresden
2021-2025
Technische Universität Dresden
2019-2025
National Center for Genetic Engineering and Biotechnology
2024
Max Planck Institut für Zellbiologie
2022
Institute of Molecular Biology
2017
Max Planck Institute for Biophysical Chemistry
2011-2016
Max Planck Society
2011-2015
Osnabrück University
2009-2012
Coherent (United States)
2012
Stressed cells shut down translation, release mRNA molecules from polysomes, and form stress granules (SGs) via a network of interactions that involve G3BP. Here we focus on the mechanistic underpinnings SG assembly. We show that, under non-stress conditions, G3BP adopts compact auto-inhibited state stabilized by electrostatic intramolecular between intrinsically disordered acidic tracts positively charged arginine-rich region. Upon unfolded mRNAs outcompete auto-inhibitory interactions,...
Keeping the noise down Protein concentrations in a cell fluctuate considerably because of stochasticity gene expression and variations cell's microenvironment. How cells cope with concentration fluctuations when precision is important unclear. Klosin et al. used combination theoretical experimental work to demonstrate that phase-separated compartments can effectively reduce protein (see Perspective by Riback Brangwynne). The results suggest phase separation provides mechanism enhance...
Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study living cells organisms. Unfortunately, applicability microscope limited, since diffraction imposes limitations on spatial resolution image. Consequently details of, for example, cellular protein distributions, can be visualized only certain extent. Fortunately, recent years have witnessed...
The eukaryotic cell membrane is connected to a dense actin rich cortex. We present FCS and STED experiments showing that bound networks have severe influence on lipid phase separation. A minimal cortex was supported bilayer via biotinylated streptavidin complexes (pinning sites). In general, binding ternary membranes prevented macroscopic liquid-ordered liquid-disordered domain formation, even at low temperature. Instead, depending the type of pinning lipid, an correlated multi-domain...
Stimulated emission depletion (STED) microscopy is routinely used to resolve the ultrastructure of cells with a ∼10-fold higher resolution compared diffraction limited imaging. While STED based on preparing excited state fluorescent probes light, recently developed expansion (ExM) provides subdiffraction by physically enlarging sample before microscopy. The fixed cross-linking and swelling hydrogels easily enlarges ∼4-fold hence increases effective optical this factor. To overcome current...
Abstract In eukaryotes, DNA is packed inside the cell nucleus in form of chromatin, which consists DNA, proteins such as histones, and RNA. Euchromatin, permissive for transcription, spatially organized into transcriptionally inactive domains interspersed with pockets transcriptional activity. While transcription RNA have been implicated euchromatin organization, it remains unclear how their interplay forms maintains pockets. Here we combine theory experiment to analyze dynamics organization...
Abstract Three-dimensional cell and organoid cultures rely on the mechanical support of viscoelastic matrices. However, commonly used matrix materials lack control over key cell-instructive properties. Here we report fully synthetic hydrogels based DNA libraries that self-assemble with ultrahigh-molecular-weight polymers, forming a dynamic DNA-crosslinked (DyNAtrix). DyNAtrix enables computationally predictable systematic its viscoelasticity, thermodynamic kinetic parameters by changing...
SUMMARY Cytosolic aggregation of the nuclear protein TDP-43 is associated with many neurodegenerative diseases, but triggers for are still debated. Here, we demonstrate that requires a double event. One up-concentration in stress granules beyond threshold, and other oxidative stress. These two events collectively induce intra-condensate demixing, giving rise to dynamic enriched phase within granules, which subsequently transitions into pathological aggregates. Mechanistically, demixing...
Heat-shocked cells prioritize the translation of heat shock (HS) mRNAs, but underlying mechanism is unclear. We report that HS in budding yeast induces disassembly eIF4F complex, where eIF4G and eIF4E assemble into translationally arrested mRNA ribonucleoprotein particles (mRNPs) granules (HSGs), whereas eIF4A promotes translation. Using vitro reconstitution biochemistry, we show a conformational rearrangement thermo-sensing eIF4A-binding domain dissociates assembly with HS-mRNPs, which...
Biomolecular condensates enable cell compartmentalization by acting as membraneless organelles
Most proteins have uneven distributions in the plasma membrane. Broadly speaking, this may be caused by mechanisms specific to each protein, or a consequence of general pattern that affects distribution all membrane proteins. The latter hypothesis has been difficult test past. Here, we introduce several approaches based on click chemistry, through which study living cells, as well sheets. We found form multi-protein assemblies are long lived (minutes), and protein diffusion is restricted....
The clustering of proteins and lipids in distinct microdomains is emerging as an important principle for the spatial patterning biological membranes. Such domain formation can be result hydrophobic ionic interactions with membrane well specific protein-protein interactions. Here using plasma membrane-resident SNARE model, we show that mismatch between length transmembrane domains (TMDs) thickness lipid suffices to induce proteins. Even when TMDs differ by only a single residue, segregate...
We have developed a bright, photostable, and far-red emitting fluorescent phosphoglycerolipid analogue to probe diffusion characteristics of lipids in membranes. The lipid consists saturated (C18) phosphoethanolamine hydrophilic dye (KK114) that is tethered the head group by long polyethylenglycol linker. In contrast reported analogues, this one partitions predominantly into liquid ordered domains phase-separated ternary bilayers. performed fluorescence correlation spectroscopy with...
The organization of proteins and lipids in the plasma membrane has been subject a long-lasting debate. Membrane rafts higher lipid chain order were proposed to mediate protein interactions, but have thus far not directly observed. Here we use micropatterning combined with single-molecule tracking put current models test: rearranged lipid-anchored raft (glycosylphosphatidylinositol(GPI)-anchored-mGFP) live cell measured effect on local environment. Intriguingly, this treatment does neither...
Formation of fluid-filled lumina by epithelial tissues is essential for organ development. How cells control the hydraulic and cortical forces to lumen morphology not well understood. Here, we quantified mechanical role tight junctions in formation using MDCK-II cysts. We found that paracellular ion barrier formed claudin receptors required inflation a lumen. However, depletion zonula occludens scaffold resulted collapse folding apical membranes. Combining quantitative measurements...