A5048983251- Cancer Cells and Metastasis
- Estrogen and related hormone effects
- Phytoestrogen effects and research
- Melanoma and MAPK Pathways
- Advanced Breast Cancer Therapies
- Cancer-related Molecular Pathways
- Cancer Treatment and Pharmacology
- Chemokine receptors and signaling
- Cancer-related molecular mechanisms research
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- Histone Deacetylase Inhibitors Research
- Synthesis and biological activity
- Sphingolipid Metabolism and Signaling
- Cellular Mechanics and Interactions
- Effects and risks of endocrine disrupting chemicals
- Cancer Research and Treatments
- Microtubule and mitosis dynamics
- Cytokine Signaling Pathways and Interactions
- Protein Kinase Regulation and GTPase Signaling
- Epigenetics and DNA Methylation
- HER2/EGFR in Cancer Research
- Cancer Mechanisms and Therapy
- Cancer Genomics and Diagnostics
- Cancer, Hypoxia, and Metabolism
Tulane University
2016-2025
Tulane Medical Center
2012-2024
University of New Orleans
2001-2022
Indiana University – Purdue University Indianapolis
2019
University of Pittsburgh Medical Center
2019
UPMC Hillman Cancer Center
2019
University of Würzburg
2019
Indiana University School of Medicine
2011-2019
German University in Cairo
2019
Duquesne University
2019
The mouse heme oxygenase-1 (HO-1) gene,<i>ho-1,</i> contains two inducible enhancers, E1 and E2. Of several cell lines tested, induction of an E1/luciferase fusion construct, pE1-luc, by CdCl<sub>2</sub> is most pronounced in MCF-7 cells. In these cells, E1, but not E2, necessary sufficient for <i>ho-1</i> gene activation. Exposure cells to 10 μm stimulates phosphorylation ERK, JNK, p38 mitogen-activated protein kinases, implicating one or more signaling pathways <i>ho-1</i>gene induction....
Unprecedented agricultural intensification and increased crop yield will be necessary to feed the burgeoning world population, whose global food demand is projected double in next 50 years. Although grain production has doubled past four decades, largely because of widespread use synthetic nitrogenous fertilizers, pesticides, irrigation promoted by "Green Revolution," this rate output unsustainable declining yields environmental impacts modern practices. The last 20 years have seen...
Abstract Introduction Of the more than one million global cases of breast cancer diagnosed each year, approximately fifteen percent are characterized as triple-negative, lacking estrogen, progesterone, and Her2/neu receptors. Lack effective therapies, younger age at onset, early metastatic spread have contributed to poor prognoses outcomes associated with these malignancies. Here, we investigate ability histone deacetylase inhibitor panobinostat (LBH589) selectively target triple-negative...
Characteristic of both chronic wounds and acute that fail to heal are excessive leukocytosis reduced matrix deposition. Estrogen is a major regulator wound repair can reverse age-related impaired healing in human animal models, characterized by dampened inflammatory response increased deposited at the site. Macrophage migration inhibitory factor (MIF) candidate proinflammatory cytokine involved hormonal regulation inflammation. We demonstrate MIF upregulated distinct spatial temporal pattern...
In the lactating breast, ERBB4 localizes to nuclei of secretory epithelium while regulating activities signal transducer and activator transcription (STAT) 5A factor essential for milk-gene expression. We have identified an intrinsic NLS (residues 676–684) within intracellular domain (4ICD) that is nuclear accumulation 4ICD. To determine functional significance 4ICD translocation in a physiologically relevant system, we demonstrated cotransfection STAT5A human breast cancer cell line...
Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris alfalfa sprout (Medicago sativa mung (Vigna radiata kudzu root (Pueraria lobata and red clover blossom (Trifolium pratense L.). Extracts of showed significant competitive binding to estrogen receptor beta (ERbeta). Estrogenic activity was determined using an estrogen-dependent MCF-7 breast cancer cell proliferation assay. Kudzu...
The steady increase in the incidence of obesity among adults has been paralleled with higher levels obesity-associated breast cancer. While recent studies have suggested that adipose stromal/stem cells (ASCs) isolated from obese women enhance tumorigenicity, mechanism(s) by which this occurs remains undefined. Evidence suggests increased adiposity results leptin secretion tissue, shown to cancer cell proliferation. Previously, our group demonstrated ASCs (obASCs) also express relative lean...
Abstract Introduction The pineal gland hormone, melatonin, has been shown by numerous studies to inhibit the proliferation of estrogen receptor α (ERα)-positive breast cancer cell lines. Here, we investigated role melatonin in regulation invasion. Methods Three invasive MCF-7 clones - MCF-7/6, MCF-7/Her2.1, and MCF-7/CXCR4 cells were employed these studies. All three lines exhibited elevated phosphorylation ERK1/2 p38 mitogen-activated protein kinase (MAPK) as determined Western blot...
Estrogen independence and progression to a metastatic phenotype are hallmarks of therapeutic resistance mortality in breast cancer patients. Metastasis has been associated with chemokine signaling through the SDF-1-CXCR4 axis. Thus, development estrogen endocrine therapy patients may be driven by signaling. Here we report that CXCR4 overexpression is indeed correlated worse prognosis decreased patient survival irrespective status receptor (ER). Constitutive activation poorly MCF-7 cells led...
Epstein-Barr virus (EBV) is associated with roughly 10% of gastric carcinomas worldwide (EBVaGC). Although previous investigations provide a strong link between EBV and carcinomas, these studies were performed using selected gene probes. Using cohort carcinoma RNA-seq data sets from The Cancer Genome Atlas (TCGA), we quantitative global assessment expression in assessed cellular pathway alterations. transcripts detected 17% samples but varied significantly coverage depth. In four the highest...
Resistance to chemotherapy remains a significant obstacle in the treatment of hormone- independent breast cancer. Recent evidence suggests that altered sphingolipid signaling through increased sphingosine kinase activity may be an important mediator cancer drug resistance. Sphingosine kinase-1 (Sphk1) is proposed key regulator tumorigenesis, proliferation and There is, however, conflicting data on role kinase-2 (Sphk2) biology resistance, with some suggesting Sphk2 has opposing Sphk1. Here,...
Background Several environmental agents termed "endocrine disrupting compounds" or EDCs have been reported to bind and activate the estrogen receptor-α (ER). The DDT BPA are ubiquitously present in environment, levels human blood adipose tissue detectable most if not all women men. ER-mediated biological responses can be regulated at numerous levels, including expression of coding RNAs (mRNAs) more recently non-coding (ncRNAs). Of ncRNAs, microRNAs emerged as a target signaling. Given...
Tamoxifen is the most commonly prescribed therapy for patients with estrogen receptor (ER)α-positive breast tumors. Tumor resistance to tamoxifen remains a serious clinical problem especially in tumors that also overexpress human epidermal growth factor 2 (HER2). Current preclinical models of HER2 overexpression fail recapitulate spectrum endocrine associated HER2/ER-positive Here, we show ectopic expression clinically important oncogenic isoform HER2, HER2Δ16, which expressed >30%...
Exposure of humans to the endocrine disrupter bisphenol A (BPA) has been associated with increased weight and obesity. However, mechanism(s) by which BPA increases adipose tissue in remains be determined. The goal this study was determine effects on adipogenesis cultured human stromal/stem cells (ASCs), precursors mature adipocytes. ASCs from three donors were for either 14 or 21 days adipogenic differentiation media containing increasing concentrations (100 pM–10 μM). extent assessed...
// Lyndsay V. Rhodes 4, * , Elizabeth C. Martin 1, H. Chris Segar 1 David F. B. Miller 3 Aaron Buechlein 5 Douglas Rusch Kenneth P. Nephew Matthew E. Burow 2 Bridgette M. Collins-Burow Department of Medicine, Section Hematology and Medical Oncology, Tulane University, New Orleans, LA, USA Pharmacology, Sciences Cellular Integrative Physiology, Indiana University School Bloomington, IN, 4 Biological Sciences, Florida Gulf Coast Fort Myers, FL, Center for Genomics Bioinformatics, These authors...
Obesity has been associated with increased incidence and mortality of breast cancer. While the precise correlation between obesity cancer remains to be determined, recent studies suggest that adipose tissue stem cells (ASCs) influence tumorigenesis tumor progression.Breast lines were co-cultured ASCs (n = 24), categorized based on site origin body mass index (BMI), assessed for enhanced proliferation, alterations in gene expression profile PCR arrays, immunocompromised mice. The was assess...