A5088452464- Reconstructive Surgery and Microvascular Techniques
- Mitochondrial Function and Pathology
- Wound Healing and Treatments
- Genetic Neurodegenerative Diseases
- RNA Research and Splicing
- Bone fractures and treatments
- RNA and protein synthesis mechanisms
- Tissue Engineering and Regenerative Medicine
- Traumatic Brain Injury and Neurovascular Disturbances
- Mesenchymal stem cell research
- DNA Repair Mechanisms
- Iron Metabolism and Disorders
- Orthopaedic implants and arthroplasty
- Dental materials and restorations
- Muscle Physiology and Disorders
- Diabetic Foot Ulcer Assessment and Management
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Developmental Biology and Gene Regulation
- Molecular Biology Techniques and Applications
- Silk-based biomaterials and applications
- Dental Implant Techniques and Outcomes
- Cancer, Hypoxia, and Metabolism
- Hemoglobinopathies and Related Disorders
- Knee injuries and reconstruction techniques
- Cancer Cells and Metastasis
Tulane University
2012-2023
Office of Diversity and Inclusion
2019
Clinical Orthopaedics and Related Research
2019
Louisiana State University Health Sciences Center New Orleans
2001-2010
Louisiana State University
2007
Expansion of an unstable GAA·TTC repeat in the first intron FXN gene causes Friedreich ataxia by reducing frataxin expression. Deficiency frataxin, essential mitochondrial protein, leads to progressive neurodegeneration and cardiomyopathy. The degree reduction correlates with tract length, but mechanism remains controversial. Here we show that transcription extensive RNA·DNA hybrid formation on templates bacteria as well defined reactions using T7 RNA polymerase vitro. hybrids can also form...
In mammals, macrophages are known to play a major role in tissue regeneration. They contribute inflammation, histolysis, re-epithelialization, revascularization and cell proliferation. Macrophages have been shown be essential for regeneration salamanders fish, but their has not elucidated mammalian epimorphic Here, using the regenerating mouse digit tip as model, we demonstrate that process. Using cell-depletion strategies, show is completely inhibited; bone histolysis does occur, wound...
Novel classes of antibiotics and new strategies to prevent treat infections are urgently needed because the rapid rise in drug-resistant bacterial recent decades has been accompanied by a parallel decline development antibiotics. Membrane permeabilizing antimicrobial peptides (AMPs) have long considered potentially promising, novel class antibiotic, especially for wound protection treatment serious infections. Yet, despite thousands known examples, AMPs only infrequently proceeded as far...
Abstract Self-renewal and differentiation of skeletal stem progenitor cells (SSPCs) are tightly regulated processes, with SSPC dysregulation leading to progressive bone disease. While the application single-cell RNA sequencing (scRNAseq) field has led major advancements in our understanding heterogeneity, by their neighboring which comprise marrow niche. However, unbiased interrogation these at transcriptional level within native niche environment been challenging. Here, we combined spatial...
Ferritin plays an important role in the storage and release of iron, element utilized cellular processes such as respiration, gene regulation, DNA replication repair. animals is composed 24 ferritin L (FTL) H (FTH) subunits ratios that vary different cell types. Because are not functionally interchangeable, both units critical for maintaining iron homeostasis protecting against overload. FTL FTH regulated primarily at a post-transcriptional level response to concentrations. Individual...
Mammalian digit regeneration progresses through consistent stages: histolysis, inflammation, epidermal closure, blastema formation, and finally redifferentiation. What we do not yet know is how each stage can affect others. Questions of timing, tissue interactions, microenvironmental states are becoming increasingly important as look toward solutions for whole limb regeneration. This study focuses on the timing closure which, in mammals, delayed compared to more regenerative animals like...
De novo limb regeneration after amputation is restricted in mammals to the distal digit tip. Central this regenerative process blastema, a heterogeneous population of lineage-restricted, dedifferentiated cells that ultimately orchestrates amputated bone and surrounding soft tissue. To investigate skeletal regeneration, we made use spatial transcriptomics characterize transcriptional profile specifically within blastema. Using technique, generated gene signature with high specificity for...
Trinucleotide repeat expansion is the genetic basis for a sizeable group of inherited neurological and neuromuscular disorders. Friedreich ataxia (FRDA) relentlessly progressive neurodegenerative disorder caused by GAA·TTC in first intron FXN gene. The expanded reduces mRNA expression length tract proportional to disease severity. Somatic sequence disease-relevant tissues thought contribute progression severity during patient aging. Previous models instability have not been able produce...
ABSTRACT Amputation of the digit tip within terminal phalangeal bone rodents, monkeys, and humans results in near-perfect regeneration surrounding tissues; however, amputations at a more proximal level fail to produce same regenerative result. Digit is coordinated, multifaceted process that incorporates signaling from bioactive growth factors both tissue matrix several different cell populations. To elucidate mechanisms involved we developed novel multi-tissue slice-culture model regenerates...
Oxygen is critical for optimal bone regeneration. While axolotls and salamanders have retained the ability to regenerate whole limbs, mammalian regeneration restricted distal tip of digit (P3) in mice, primates, humans. Our previous study revealed oxygen microenvironment during dynamic temporally influential building degrading bone. Given that dependent on a changing environment, better understanding effects wounding, scarring, regeneration, ways artificially generate both hypoxic replete...
Increased prevalence of antibiotic resistance in skin and soft tissue infections is a concerning public health challenge currently facing medical science. A combinatory, broad spectrum biocidal antiseptic has been developed ('ASP') as topically-applied solution to potential resistant polymicrobial infected wounds that may be encountered this context. The ASP-105 designate was evaluated vitro by determining the minimal inhibitory concentration (MIC) bactericidal (MBC), against different...
The regeneration blastema which forms following amputation of the mouse digit tip is composed undifferentiated cells bound together by an organized network fibers. A monoclonal antibody (ER-TR7) that identifies extracellular matrix (ECM) fibers produced fibroblast reticular during lymphoid organogenesis was used to characterize ECM digit, blastema, and regenerate. Digit produce ER-TR7+ associated with different tissues represent a subset loose connective tissue fibroblasts. During formation...
There are an insufficient number of donor organs available to meet the demand for lung transplantation. This issue could be addressed by regenerating functional tissue from diseased or damaged lungs that would otherwise deemed unsuitable transplant. Detergent-mediated whole-lung decellularization produces a three-dimensional natural scaffold can repopulated with various cell types. In this study, we investigated and initial recellularization using rat model monocrotaline-induced pulmonary...
ABSTRACT In mammals, macrophages are known to play a major role in tissue regeneration. These cells contribute inflammation, histolysis, re-epithelialization, re-vascularization and cell proliferation. While have been shown be essential for epimorphic regeneration salamanders fish, their has not elucidated mammalian Here, using the mouse digit tip model as of regeneration, we demonstrate that process. Using depletion strategies, show is completely inhibited; bone histolysis does occur, wound...
Acute hemorrhage commonly leads to coagulopathy and organ dysfunction or failure. Recent evidence suggests that damage the endothelial glycocalyx contributes these adverse outcomes. The physiological events mediating acute shedding are undefined, however. Here, we show succinate accumulation within cells drives degradation through a membrane reorganization-mediated mechanism. We investigated this mechanism in cultured cell hypoxia-reoxygenation model, rat model of hemorrhage, trauma patient...
Background Making the correct choice between transcription elongation and termination is essential to function of RNA polymerase II, fundamental gene expression. This can be influenced by factors modifying complex, chromatin, or signals mediated template transcript. To aid in study we have developed a reporter system that consists tandem luciferase reporters flanking test sequence interest. The ratio expression from provides measure relative rates successful through intervening sequence....
Abstract The mitochondrial deacetylase sirtuin 3 (SIRT3) is thought to be one of the main contributors metabolic flexibility–promoting energy production and maintaining homeostasis. In bone, profiles are tightly regulated loss SIRT3 has deleterious effects on bone volume in vivo osteoblast differentiation vitro . Despite prominent role this protein stem cell proliferation, activity, differentiation, importance for regeneration after injury never been reported. We show here, using mouse digit...
Abstract In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially nonengraftment as well. MSCs are a mixture of progenitors at different stages cellular aging, but the contribution this heterogeneity MSC implants unknown. Here, we employ biomarker decoy TRAIL receptor CD264, compare kinetics two populations in human bone marrow (hBM‐MSC) cultures. Sorted CD264 + hBM‐MSCs from age‐matched donors have elevated β‐galactosidase...
Mammalian calvarium is composed of flat bones developed from two origins, neural crest, and mesoderm. Cells both origins exhibit similar behavior but express distinct transcriptomes. It intriguing to ask whether genes shared by play or roles in development. In the present study, we have examined role Pdgfra, which expressed crest mesoderm, specific lineages during calvarial
Cell metabolism coordinates the biochemical reactions that produce carbon and ATP in order for cell to proliferate, differentiate, respond environmental changes. type determines metabolic demand, so proliferating skeletal progenitors differentiated osteoblasts exhibit different levels of metabolism. Limb regeneration is an energetically demanding process involves multiple types tissues functions over time. Dysregulation aged mice results impaired regeneration, a defect can be rescued part by...
Mouse digit amputation provides a useful model of bone growth after injury, in that the injury promotes intramembranous formation an adult animal. The tip is composed skin, nerves, blood vessels, bones, and tendons, all which regenerate amputation, making it powerful for multi-tissue regeneration. Bone integrity relies upon balanced remodeling between resorption formation, which, when disrupted, results changes to architecture biomechanics, particularly during aging. In this study, we used...