A5029788171- Hair Growth and Disorders
- Wound Healing and Treatments
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Nerve injury and regeneration
- Renal and related cancers
- Immune cells in cancer
- Mosquito-borne diseases and control
- Vitamin D Research Studies
- RNA regulation and disease
- Vitamin C and Antioxidants Research
- Soft tissue tumor case studies
- Genetic Syndromes and Imprinting
- Malaria Research and Control
- Heterotopic Ossification and Related Conditions
- Adipokines, Inflammation, and Metabolic Diseases
- Single-cell and spatial transcriptomics
- Lymphatic System and Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- HIV Research and Treatment
- Cancer Cells and Metastasis
- Immune Response and Inflammation
The University of Texas Southwestern Medical Center
2023-2025
University of California, Los Angeles
2017-2022
Broad Center
2017-2022
University of Miami
2019-2022
Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are a promising system for studying the distinct features of developing brain and underlying causes many neurological disorders. While organoid technology is steadily advancing, challenges remain, including potential batch-to-batch cell-line-to-cell-line variability, structural inconsistency. Here, we demonstrate that major contributor to cortical quality way hPSCs maintained prior differentiation. Optimal results...
Modulation of the immune response to initiate and halt inflammatory process occurs both at site injury as well systemically. Due evolving role cellular metabolism in regulating cell fate function, tendon injuries which undergo normal aberrant repair were evaluated by metabolic profiling determine its impact on healing outcomes. Metabolomics revealed an increasing abundance immunomodulatory metabolite itaconate with site. Subsequent single-cell RNA sequencing, molecular metabolomic validation...
Abstract Self-renewal and differentiation of skeletal stem progenitor cells (SSPCs) are tightly regulated processes, with SSPC dysregulation leading to progressive bone disease. While the application single-cell RNA sequencing (scRNAseq) field has led major advancements in our understanding heterogeneity, by their neighboring which comprise marrow niche. However, unbiased interrogation these at transcriptional level within native niche environment been challenging. Here, we combined spatial...
Somatic activating mutations in KRAS can cause complex lymphatic anomalies (CLAs). However, the specific processes that drive KRAS-mediated CLAs have yet to be fully elucidated. Here, we used single-cell RNA sequencing construct an atlas of normal and KrasG12D-malformed vessels. We identified six subtypes endothelial cells (LECs) lungs adult wild-type mice (Ptx3, capillary, collecting, valve, mixed, proliferating). To determine when LEC were specified during development, integrated our data...
Abstract Glial-cell-derived neurotrophic factor (GDNF) is a well-studied neuroregenerative factor; however, the degree to which it supports hair formation and skin wound repair not known. By using Gfra1 (GDNF family receptor alpha 1) knock-in reporter mouse line, GDNF signaling was found occur within bulge stem cells (BSCs) during initiation of cycle early stages after depilation. Both recombinant transgene overexpression promoted BSC colony growth, formation, wounding through enhanced...
MicroRNAs (miRNAs) are a major class of conserved non-coding RNAs that have wide range functions during development and disease. Biogenesis canonical miRNAs depend on the cytoplasmic processing pre-miRNAs to mature by Dicer endoribonuclease. Once generated, miRNA-induced silencing complex, or miRISC, incorporates one strand as template for recognizing complementary target messenger (mRNAs) dictate post-transcriptional gene expression. Besides regulating miRNA biogenesis, is also part miRISC...
Heterotopic ossification (HO) is a challenging condition that occurs after musculoskeletal injury and characterized by the formation of bone in non-skeletal tissues. While effect HO on blood vessels well established, little known about its impact lymphatic vessels. Here, we use mouse model traumatic to investigate relationship between We show triggers lymphangiogenesis at site, which associated with elevated vascular endothelial growth factor C (VEGF-C) levels. Through single-cell...
SUMMARY Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are emerging as an effective system to study the distinct features of developing brain and underlying causes many neurological disorders. While progress in organoid technology has been steadily advancing, challenges remain including rampant batch-to-batch cell line-to-cell line variability irreproducibility. Here, we demonstrate that a major contributor successful cortical production is manner which hPSCs...
Abstract We propose that GDNF, a glial cell line‐derived neurotrophic factor, can promote hair follicle neogenesis and skin regeneration after wounding by directing the fate of dermal fibroblasts. Our hypothesis is largely based on detailed GDNF receptor analysis during regenerative stages, as well induction receptors between pro‐regenerative spiny mouse (genus Acomys ) its less‐regenerative descendant, house ( Mus musculus ). To characterize GDNF‐target cells, we will conduct series...
Abstract MicroRNAs (miRNAs) are a major class of conserved non-coding RNAs that have wide range functions during development and disease. Biogenesis canonical miRNAs depend on the cytoplasmic processing pre-miRNAs to mature by Dicer endoribonuclease. Once generated, miRNA-induced silencing complex, or miRISC, incorporates one strand as template for recognizing complementary target messenger (mRNAs) dictate post-transcriptional gene expression. Besides regulating miRNA biogenesis, is also...