A5064932750- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Bone fractures and treatments
- Heterotopic Ossification and Related Conditions
- Sarcoma Diagnosis and Treatment
- Hedgehog Signaling Pathway Studies
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Axon Guidance and Neuronal Signaling
- Bone Metabolism and Diseases
- Cancer-related gene regulation
- Cleft Lip and Palate Research
- Genetic Syndromes and Imprinting
- Connective tissue disorders research
- Soft tissue tumor case studies
- Bone Tumor Diagnosis and Treatments
- Craniofacial Disorders and Treatments
- Spine and Intervertebral Disc Pathology
- Osteoarthritis Treatment and Mechanisms
- Oral and Maxillofacial Pathology
- dental development and anomalies
- TGF-β signaling in diseases
- Tendon Structure and Treatment
- Wound Healing and Treatments
- Soft tissue tumors and treatment
Johns Hopkins University
2016-2025
Johns Hopkins Medicine
2020-2025
Bharati Vidyapeeth Deemed University
2024
University of California, Los Angeles
2013-2023
Orthopaedic Hospital
2013-2023
University of Baltimore
2023
University Hospitals Plymouth NHS Trust
2023
Johns Hopkins Hospital
2022
Yale University
2021
Orthopaedic Research and Education Foundation
2020
The fetal skeleton arises from neural crest and mesoderm. Here, we provide evidence that each lineage contributes a unique stem cell population to the regeneration of injured adult bones. Using Wnt1Cre::Z/EG mice found crest-derived mandible heals with skeletal cells, whereas mesoderm-derived tibia cells. We tested whether cells were functionally interchangeable by grafting into mandibular defects, vice versa. All scenarios, except one, healed through direct differentiation osteoblasts; when...
Background Human adipose-derived stromal cells (hASCs) represent a multipotent cell type with proven capacity to differentiate along an osteogenic lineage. This suggests that they may be used heal defects of the craniofacial or appendicular skeleton. We sought substantiate use undifferentiated hASCs in regeneration non-healing mouse skeletal defect. Methodology/Principal Findings ASCs were harvested from female lipoaspirate. Critical-sized (4 mm) calvarial created parietal bone adult male...
Bone is richly innervated by nerve growth factor–responsive (NGF-responsive) tropomyosin receptor kinase A–expressing (TrKa-expressing) sensory fibers, which are required for osteochondral progenitor expansion during mammalian skeletal development. Aside from pain sensation, little known regarding the role of innervation in bone repair. Here, we characterized reinnervation tissue following experimental ulnar stress fracture and assessed impact loss TrkA signaling this process. Sequential...
Abstract Heterotopic ossification (HO) is an aberrant regenerative process with ectopic bone induction in response to musculoskeletal trauma, which mesenchymal stem cells (MSC) differentiate into osteochondrogenic instead of myocytes or tenocytes. Despite frequent cases hospitalized the inflammatory responses and cell population dynamics that regulate subsequent wound healing tissue regeneration are still unclear. Here we examine, using a mouse model trauma-induced HO, local microenvironment...
Abstract Adipose tissue is an ideal source of mesenchymal stem cells for bone engineering: it largely dispensable and readily accessible with minimal morbidity. However, the stromal vascular fraction (SVF) adipose a heterogeneous cell population, which leads to unreliable formation. In present study, we prospectively purified human perivascular (PSCs) from compared their bone-forming capacity that traditionally derived SVF. PSCs are population (sorted by fluorescence-activated sorting)...
Clinically available sources of bone for repair and reconstruction are limited by the accessibility autologous grafts, infectious risks cadaveric materials, durability synthetic substitutes. Cell-based approaches skeletal regeneration can potentially fill this need, adipose tissue represents a promising source development such therapies. Here, we enriched an osteogenic subpopulation cells derived from human subcutaneous utilizing microfluidic-based single cell transcriptional analysis...
Human faces exhibit enormous variation. When pathological conditions are superimposed on normal variation, a nearly unbroken series of facial morphologies is produced. viewed in full, this spectrum ranges from cyclopia and hypotelorism to hypertelorism duplications. Decreased Hedgehog pathway activity causes holoprosencephaly hypotelorism. Here, we show that excessive activity, caused by truncating the primary cilia cranial neural crest cells, frontonasal dysplasia (FND). Elimination...
Adipose-derived stromal cells (ASCs) present a great potential for tissue engineering, as they are capable of differentiating into osteogenic and adipogenic cell types, among others. In this study, we examined the role Hedgehog signaling in balance differentiation mouse ASCs. Results showed that increased during early (Shh, Ptc1, Gli1), but decreased differentiation. N-terminal Sonic (Shh-N) significantly vitro ASCs, by all markers (*p < 0.01). Concomitantly, Shh-N abrogated differentiation,...
Abstract Adipose tissue is an ideal mesenchymal stem cell (MSC) source, as it dispensable and accessible with minimal morbidity. However, the stromal vascular fraction (SVF) of adipose a heterogeneous population, which has disadvantages for regeneration. In present study, we prospectively purified human perivascular cells (PSCs) from n = 60 samples lipoaspirate documented their frequency, viability, variation patient demographics. PSCs are fluorescence-activated sorting-sorted population...
Abstract NELL-1 is a secreted, osteoinductive protein whose expression rheostatically controls skeletal ossification. Overexpression of results in craniosynostosis humans and mice, whereas lack Nell-1 associated with undermineralization. Here we show that -haploinsufficient mice have normal development but undergo age-related osteoporosis, characterized by reduction osteoblast:osteoclast (OB:OC) ratio increased bone fragility. Recombinant binds to integrin β1 consequently induces...
Ewing's sarcoma is a rare and aggressive malignancy. In the present study, tumor from patient with cyclin-dependent kinase inhibitor 2A/B (CDKN2A/B) loss FUS-ERG fusion was implanted in right chest wall of nude mice to establish patient-derived orthotopic xenograft (PDOX) model. The aim study determine efficacy 4/6 (CDK4/6) insulin-like growth factor-1 receptor (IGF-1R) inhibitors on PDOX. PDOX models were randomized into following groups when volume reached 50 mm3: G1, untreated control;...