A5083744064- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Immune cells in cancer
- Glioma Diagnosis and Treatment
- Wound Healing and Treatments
- Histone Deacetylase Inhibitors Research
- Cancer, Hypoxia, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Reconstructive Surgery and Microvascular Techniques
- Bone fractures and treatments
- Cell Adhesion Molecules Research
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Radiopharmaceutical Chemistry and Applications
- Nanoplatforms for cancer theranostics
- Pancreatic and Hepatic Oncology Research
- Cleft Lip and Palate Research
- Single-cell and spatial transcriptomics
- Wnt/β-catenin signaling in development and cancer
- Breast Implant and Reconstruction
- Cancer-related gene regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Body Contouring and Surgery
- Angiogenesis and VEGF in Cancer
- Bone and Dental Protein Studies
University of California, Santa Barbara
2025
The Ohio State University
2025
Neurological Surgery
2017-2024
University of California, San Francisco
2017-2024
Stanford University
2018-2024
Cedars-Sinai Medical Center
2024
Charles R. Drew University of Medicine and Science
2024
UCSF Helen Diller Family Comprehensive Cancer Center
2019-2022
Oakland University
2020-2022
Rowan University
2022
Significance In the skin, tissue injury results in fibrosis form of a scar composed dense extracellular matrix deposited by fibroblasts. Therapies that promote regeneration rather than remain elusive because principles fibroblast programming and response to incompletely understood. Here, we present multimodal -omics platform for study cell populations complex tissue, which has allowed us characterize wound healing fibroblasts across both time space. We identify functionally distinct...
Cancer-associated fibroblasts (CAFs) were presumed absent in glioblastoma given the lack of brain fibroblasts. Serial trypsinization specimens yielded cells with CAF morphology and single-cell transcriptomic profiles based on their copy number variations (CNVs) elevated individual cell probability scores derived from expression 9 markers absence 5 non-CAF stromal sharing features CAFs. Cells without CNVs high identified RNA-Seq 12 patient glioblastomas. Pseudotime reconstruction revealed...
Abstract Adipose tissue is an ideal mesenchymal stem cell (MSC) source, as it dispensable and accessible with minimal morbidity. However, the stromal vascular fraction (SVF) of adipose a heterogeneous population, which has disadvantages for regeneration. In present study, we prospectively purified human perivascular cells (PSCs) from n = 60 samples lipoaspirate documented their frequency, viability, variation patient demographics. PSCs are fluorescence-activated sorting-sorted population...
Adhesions are fibrotic scars that form between abdominal organs following surgery or infection, and may cause bowel obstruction, chronic pain, infertility. Our understanding of adhesion biology is limited, which explains the paucity anti-adhesion treatments. Here we present a systematic analysis mouse human tissues. First, show adhesions derive primarily from visceral peritoneum, consistent with our clinical experience laparotomy rather than laparoscopy. Second, formed by poly-clonal...
The molecular underpinnings of invasion, a hallmark cancer, have been defined in terms individual mediators but crucial interactions between these remain undefined. In xenograft models and patient specimens, we identified c-Met/β1 integrin complex that formed during significant invasive oncologic processes: breast cancer metastases glioblastoma resistance to antiangiogenic VEGF neutralizing antibody, bevacizumab. Inducing formation through an engineered inducible heterodimerization system...
Abstract Glioblastoma (GBM) responses to bevacizumab are invariably transient with acquired resistance. We profiled paired patient specimens and bevacizumab-resistant xenograft models pre- post-resistance toward the primary goal of identifying regulators whose targeting could prolong therapeutic window, secondary biomarkers window closure. Bevacizumab-resistant xenografts exhibited decreased vessel density increased hypoxia versus pre-resistance, suggesting that resistance occurs despite...
Pancreatic ductal adenocarcinoma (PDAC) is projected to become the second leading cause of cancer-related death. Hallmarks include desmoplasia with variable extracellular matrix (ECM) architecture and a complex microenvironment spatially defined tumor, stromal, immune populations. Nevertheless, role desmoplastic spatial organization in patient/tumor variability remains underexplored, which we elucidate using two technologies. First, quantify ECM patterning 437 patients, revealing...
Clinical trials revealed limited response duration of glioblastomas to VEGF-neutralizing antibody bevacizumab. Thriving in the devascularized microenvironment occurring after antiangiogenic therapy requires tumor cell adaptation decreased glucose, with 50% less glucose identified bevacizumab-treated xenografts. Compared bevacizumab-responsive xenograft cells, resistant cells exhibited increased uptake, glycolysis, 13C NMR pyruvate lactate conversion, and survival low glucose. Glucose...
INTRODUCTION: Glioblastoma (GBM) recurrence remains an unmet medical challenge, largely attributed to resilient glioma cancer stem cells (CSCs) that evade conventional therapies. TCR T bear virally infected cell receptors for targeting antigen-bearing tumor cells. NY-ESO-1-specific receptor (TCR) can target GBM Decitabine (DAC) increases NY-ESO-1 expression, specifically in CSCs, potentially enabling the eradication of this population. METHODS: expression was analyzed by immunocytochemistry...
INTRODUCTION: The utility of Frailty and comorbid disease indices in pediatric patients undergoing cranial tumor resection is unclear. Most these have only been validated for use adult populations, no such index currently exists neurosurgical patients. Some categories utilized indices, as fecal incontinence, weight loss, renal failure, can be assessed this population to predict post-surgical outcomes. METHODS: Nationwide Readmissions Database (NRD) was queried primary brain between 2016...
OBJECTIVE Glioblastoma (GBM) is an aggressive brain malignancy with a short overall patient survival, yet there remains significant heterogeneity in outcomes. Although access to health care has previously been linked impact on prognosis several malignancies, this question incompletely answered GBM. METHODS This study was retrospective analysis of 354 newly diagnosed patients GBM who underwent first resection at the authors' institution (2007-2015). RESULTS Of (median age 61 years, and 37.6%...
Hedgehog (Hh) signaling positively regulates both endochondral and intramembranous ossification. Use of small molecules for tissue engineering applications poses several advantages. In this study, we examined whether use an acellular scaffold treated with the molecule Smoothened agonist (SAG) could aid in critical-size mouse calvarial defect repair. First, verified pro-osteogenic effect SAG vitro, using primary neonatal cells (NMCCs). Next, a 4 mm nonhealing was created mid-parietal bone...
Abstract Fluorescent proteins are used extensively in transgenic animal models to label and study specific cell tissue types. Expression of these can be imaged analyzed using fluorescent confocal microscopy. Conventional microscopes cannot penetrate through more than 4–6 μm thick. Tissue clearing procedures overcome this challenge by rendering thick specimens into translucent tissue. However, most techniques do not satisfactorily preserve expression endogenous fluorophores. Using simple...