A5059156946- Wound Healing and Treatments
- Tendon Structure and Treatment
- Hippo pathway signaling and YAP/TAZ
- Pancreatic and Hepatic Oncology Research
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Silk-based biomaterials and applications
- Skin and Cellular Biology Research
- Glioma Diagnosis and Treatment
- Liver Disease Diagnosis and Treatment
- Antimicrobial Peptides and Activities
- Reconstructive Facial Surgery Techniques
- Medical Research and Treatments
- Pressure Ulcer Prevention and Management
- Orthopedic Surgery and Rehabilitation
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Single-cell and spatial transcriptomics
- Surgical Sutures and Adhesives
- Bone fractures and treatments
- Laser Applications in Dentistry and Medicine
- Developmental Biology and Gene Regulation
- Medical Coding and Health Information
- Neurofibromatosis and Schwannoma Cases
- Horticultural and Viticultural Research
Stanford University
2020-2025
Stanford Medicine
2020-2023
Creative Commons
2020-2021
Skin scarring, the end result of adult wound healing, is detrimental to tissue form and function. Engrailed-1 lineage-positive fibroblasts (EPFs) are known function in but lineage-negative (ENFs) remain poorly characterized. Using cell transplantation transgenic mouse models, we identified a dermal ENF subpopulation that gives rise postnatally derived EPFs by activating expression during healing. By studying responses substrate mechanics, found mechanical tension drives activation via...
Biological scaffolds such as hydrogels provide an ideal, physio-mimetic of native extracellular matrix (ECM) that can improve wound healing outcomes after cutaneous injury. While most studies have focused on the benefits in accelerating healing, there are minimal data directly comparing different hydrogel material compositions. In this study, we utilized a splinted excisional model recapitulates human-like mice and treated wounds with three collagen dressings. We assessed feasibility...
Pancreatic ductal adenocarcinoma (PDAC) is projected to become the second leading cause of cancer-related death. Hallmarks include desmoplasia with variable extracellular matrix (ECM) architecture and a complex microenvironment spatially defined tumor, stromal, immune populations. Nevertheless, role desmoplastic spatial organization in patient/tumor variability remains underexplored, which we elucidate using two technologies. First, quantify ECM patterning 437 patients, revealing...
Modulating mechanotransduction by inhibiting yes-associated protein (YAP) in mice yields wound regeneration without scarring. However, rodents are loose-skinned and fail to recapitulate key aspects of human repair. We sought elucidate the effects YAP inhibition red Duroc pig wounds, most human-like model show that one-time treatment with verteporfin, a inhibitor, immediately after wounding is sufficient prevent scarring drive pigs. By performing single-cell RNA sequencing (scRNA-seq) on...
Pancreatic ductal adenocarcinoma (PDAC) represents one of the only cancers with an increasing incidence rate and is often associated intra- peri-tumoral scarring, referred to as desmoplasia. This scarring highly heterogeneous in extracellular matrix (ECM) architecture plays complex roles both tumor biology clinical outcomes that are not yet fully understood. Using hematoxylin eosin (H&E), a routine histological stain utilized existing workflows, we quantified ECM 85 patient samples assess...
The tendon enthesis plays a critical role in facilitating movement and reducing stress within joints. Partial injuries heal mechanically inferior manner never achieve healthy tissue function. cells responsible for tendon-to-bone healing remain incompletely characterized their origin is unknown. Here, we evaluated the putative of mouse skeletal stem (mSSCs) after partial-injury. We found that mSSCs were present at elevated levels following injury these downregulated TGFβ signaling pathway...
Journal Article Confirmation of a gene for dominant dilution horse colors Get access L. D. VAN VLECK, VLECK Department Animal Science, Cornell UniversityIthaca, NY 14853 Search other works by this author on: Oxford Academic PubMed Google Scholar M. DAVITT Quarter Dale Farm451 3rd Avenue Extension, Rensselaer, 12144 Heredity, Volume 68, Issue 5, September 1977, Pages 280–282, https://doi.org/10.1093/oxfordjournals.jhered.a108836 Published: 01 1977
Summary Regeneration is the “holy grail” of tissue repair, but skin injury typically yields fibrotic, non-functional scars. Developing pro-regenerative therapies requires rigorous understanding molecular progression from to fibrosis or regeneration. Here, we report divergent events driving wound cells toward either scarring regenerative fates. We profile versus YAP inhibition-induced regeneration at transcriptional (single-cell RNA-sequencing), protein (timsTOF proteomics), and...
Purpose: Skin scars represent a massive biomedical burden on patients, but the cellular mediators of scarring remain poorly understood. We previously showed that embryonic expression Engrailed-1 (En-1) defines lineage fibroblasts (En-1-positive fibroblasts; eEPFs) responsible for deposition fibrotic scar tissue in dorsal skin. More recently, we demonstrated subpopulation En-1-negative (ENFs) activates En-1 during adult wound healing and contributes to formation as postnatally-derived EPFs...
Purpose: The “super-healing” MRL/MpJ mouse is uniquely able to regenerate ear punch wounds, while dorsal wounds heal in a typical scarring fashion. Identification of the genes responsible for regeneration these mice could provide insights toward novel anti-scarring therapies. However, previous methods identifying genetic basis species-/strain-specific traits are imprecise and extremely resource-intensive. Recent studies have revealed that, by outbreeding strain with trait interest (here,...
Summary In adult mammals, skin wounds typically heal by scarring rather than through regeneration. contrast, “super-healer” MRL mice have the unusual ability to regenerate ear punch wounds, yet molecular basis for this regeneration remains elusive. Here, in hybrid crosses between and non-regenerating mice, we use allele-specific gene expression identify cis -regulatory variation associated with Analyzing three major wound cell populations, identified extensive strain- tissue- specific cis-...
Purpose: Scarring in the mouse dorsal dermis is mediated by pro-fibrotic, Engrailed-1 lineage-positive fibroblasts (EPFs). We recently showed that mechanotransduction blockade (YAP inhibition, using drug verteporfin), results complete wound regeneration, with full recovery of normal dermal appendages (hair follicles, glands), extracellular matrix (ECM) architecture, and tensile strength. This regenerative outcome following verteporfin treatment lineage-negative (ENFs). The complex milieu...