A5022249966- Wound Healing and Treatments
- Tendon Structure and Treatment
- Single-cell and spatial transcriptomics
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Antimicrobial Peptides and Activities
- Pressure Ulcer Prevention and Management
- Skin and Cellular Biology Research
- Surgical Sutures and Adhesives
- Body Contouring and Surgery
- Cancer Cells and Metastasis
- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- Cellular Mechanics and Interactions
- Reconstructive Facial Surgery Techniques
- Inflammatory Bowel Disease
- Colorectal Cancer Surgical Treatments
- Erythrocyte Function and Pathophysiology
- Immune cells in cancer
- Silk-based biomaterials and applications
- Diabetic Foot Ulcer Assessment and Management
Stanford University
2022-2025
Institute for Stem Cell Biology and Regenerative Medicine
2023
Stanford Medicine
2022-2023
California Institute for Regenerative Medicine
2022-2023
Modulating mechanotransduction by inhibiting yes-associated protein (YAP) in mice yields wound regeneration without scarring. However, rodents are loose-skinned and fail to recapitulate key aspects of human repair. We sought elucidate the effects YAP inhibition red Duroc pig wounds, most human-like model show that one-time treatment with verteporfin, a inhibitor, immediately after wounding is sufficient prevent scarring drive pigs. By performing single-cell RNA sequencing (scRNA-seq) on...
While past studies have suggested that plasticity exists between dermal fibroblasts and adipocytes, it remains unknown whether fat actively contributes to fibrosis in scarring. We show adipocytes convert scar-forming response
Abstract Skin fibrosis is a clinical problem with devastating impacts but limited treatment options. In the setting of diabetes, insulin administration often causes local dermal fibrosis, leading to range sequelae including impeded absorption. Mechanical forces are important drivers and, clinically, physical tension offloading at skin level using an elastomeric patch significantly reduces wound scarring. However, it not known whether could similarly prevent in pro-fibrotic injections. Here,...
Summary In adult mammals, skin wounds typically heal by scarring rather than through regeneration. contrast, “super-healer” MRL mice have the unusual ability to regenerate ear punch wounds, yet molecular basis for this regeneration remains elusive. Here, in hybrid crosses between and non-regenerating mice, we use allele-specific gene expression identify cis -regulatory variation associated with Analyzing three major wound cell populations, identified extensive strain- tissue- specific cis-...
Despite its rapidly increased availability for the study of complex tissue, single-cell RNA sequencing remains prohibitively expensive large studies. Here, we present a protocol using oligonucleotide barcoding tagging and pooling multiple samples from healing wounds, which are among most challenging tissue types this application. We describe steps to generate skin wounds in mice, followed by harvest barcoding. This is also applicable other species including rats, pigs, humans. For complete...