A5026243099- Wound Healing and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Advanced Polymer Synthesis and Characterization
- Cellular Mechanics and Interactions
- Tendon Structure and Treatment
- Breast Implant and Reconstruction
- Hydrogels: synthesis, properties, applications
- Polymer Surface Interaction Studies
- Immune cells in cancer
- Planarian Biology and Electrostimulation
- Angiogenesis and VEGF in Cancer
- Dermatologic Treatments and Research
- Hippo pathway signaling and YAP/TAZ
- Aortic Disease and Treatment Approaches
- Cancer Cells and Metastasis
- Hernia repair and management
- Extracellular vesicles in disease
- Surgical Sutures and Adhesives
- Reconstructive Facial Surgery Techniques
- Pelvic and Acetabular Injuries
- Vascular Procedures and Complications
- RNA Interference and Gene Delivery
Stanford University
2015-2025
The University of Texas Southwestern Medical Center
2022-2025
University of Michigan
2023-2025
Stanford Medicine
2021-2025
University of Pretoria
2019-2024
Southwestern Medical Center
2022-2024
University of Arizona
2023-2024
Creative Commons
2018-2023
Heidelberg University
2017-2023
Palo Alto University
2020-2022
Significance In the skin, tissue injury results in fibrosis form of a scar composed dense extracellular matrix deposited by fibroblasts. Therapies that promote regeneration rather than remain elusive because principles fibroblast programming and response to incompletely understood. Here, we present multimodal -omics platform for study cell populations complex tissue, which has allowed us characterize wound healing fibroblasts across both time space. We identify functionally distinct...
Abstract Tissue repair and healing remain among the most complicated processes that occur during postnatal life. Humans other large organisms heal by forming fibrotic scar tissue with diminished function, while smaller respond scarless regeneration functional restoration. Well-established scaling principles reveal organism size exponentially correlates peak forces movement, evolutionary responses have compensated strengthening organ-level mechanical properties. How these adaptations may...
Biological scaffolds such as hydrogels provide an ideal, physio-mimetic of native extracellular matrix (ECM) that can improve wound healing outcomes after cutaneous injury. While most studies have focused on the benefits in accelerating healing, there are minimal data directly comparing different hydrogel material compositions. In this study, we utilized a splinted excisional model recapitulates human-like mice and treated wounds with three collagen dressings. We assessed feasibility...
Burns and other traumatic injuries represent a substantial biomedical burden. The current standard of care for deep is autologous split-thickness skin grafting (STSG), which frequently results in contractures, abnormal pigmentation, loss biomechanical function. Currently, there are no effective therapies that can prevent fibrosis contracture after STSG. Here, we have developed clinically relevant porcine model STSG comprehensively characterized cell populations involved healing with...
Abstract Molecular mechanisms of organismal and cell aging remain incompletely understood. We, therefore, generated a body-wide map noncoding RNA (ncRNA) expression in (16 organs at ten timepoints from 1 to 27 months) rejuvenated mice. We found molecular trajectories are largely tissue-specific except for eight broadly deregulated microRNAs (miRNAs). Their individual abundance mirrors their presence circulating plasma extracellular vesicles (EVs) whereas ncRNAs were less present. For...
Vitamin D–activated Trem2 + macrophages provide a novel strategy to develop advanced cell therapies for complex wounds.
Burn scars and scar contractures cause significant morbidity for patients. Recently, cell-based therapies have been proposed as an option improving healing reducing scarring after burn injury, through their known proangiogenic immunomodulatory paracrine effects. Our laboratory has developed a pullulan-collagen hydrogel that, when seeded with mesenchymal stem cells (MSCs), improves cell viability augments capacity in vivo. Concurrently, recent research suggests that prospective isolation of...
A high energy X-ray diffraction technique is employed in a new way to make operando through-thickness measurements inside large format commercial Li-ion pouch cell. The technique, which has sub-mm in-plane spatial resolution, simultaneously determines the local temperature, state of charge both electrodes (as opposed global average determined electrochemically), and elastic strain current collectors, all without embedding any intrusive sensors that alter battery behavior. As thermal...
The optimal time for flap anastomosis to an arteriovenous loop remains controversial. Whether perforator flaps and axially vascularized muscle or fasciocutaneous lead comparable outcomes in conjunction with loops has not been investigated.Medical records from 103 patients undergoing reconstruction (76 one-stage 27 two-stage) between 2007 2017 were reviewed. Postoperative compared one- two-stage reconstructions different types of free flaps.Rates thrombosis, major wound complications, failure...
Background: Recent advances in high-throughput single-cell sequencing technologies have led to their increasingly widespread adoption for clinical applications. However, challenges associated with tissue viability, cell yield, and delayed time-to-capture created unique obstacles data processing. Chronic wounds, particular, represent some of the most difficult target specimens, due significant amount fibrinous debris, extracellular matrix components, non-viable cells inherent routinely...
Conventional synthetic vascular grafts are associated with significant failure rates due to their mismatched mechanical properties the native vessel and poor regenerative potential. Though different tissue engineering approaches have been used improve biocompatibility of grafts, it is still crucial develop a new generation that can match dynamics direct host response achieve robust regeneration. The size pores within implanted biomaterials has shown effects on macrophage polarization, which...
Summary: Although robotic surgery has been routinely established in other surgical disciplines, technologies have less readily adopted plastic surgery. Despite a strong demand for innovation and cutting-edge technology surgery, most reconstructive procedures, including microsurgery, continued to necessitate an open approach. Recent advances robotics artificial intelligence, however, are gaining momentum shown significant promise improve patient care These next-generation robots the potential...
Abstract Small animals do not replicate the severity of human foreign-body response (FBR) to implants. Here we show that FBR can be driven by forces generated at implant surface that, owing allometric scaling, increase exponentially with body size. We found is mediated immune-cell-specific RAC2 mechanotransduction signalling, independently chemistry and mechanical properties implant, a pathological human-like molecular, cellular tissue levels induced in mice via application...
Vascular shear stress promotes endothelial cell sprouting in vitro. The impact of hemodynamic forces on microRNA (miRNA) and gene expression within growing vascular networks vivo, however, remain poorly investigated. Arteriovenous (AV) shunts are an established model for induction neoangiogenesis vivo can serve as a tool analysis effects miRNA profiles over time. AV were microsurgically created rats explanted postoperative days 5, 10 15. Neoangiogenesis was confirmed by histologic...
Abstract One of the fundamental laws in crystallization is translational symmetry, which accounts for profound shapes observed natural mineral crystals and snowflakes. Herein, we report on spontaneous formation spherical hollow with broken symmetry crystalline molecular bottlebrush (mBB) polymers. The unique structure named as mBB crystalsome (mBBC), highlighting its similarity to classical vesicles. Fluorescence resonance energy transfer (FRET) experiments show that mBBC driven by local...
Uniquely among mammalian organs, skin is capable of marked size change in adults, yet the mechanisms underlying this notable capacity are unclear. Here, we use a system controlled tissue expansion mice to uncover cellular and molecular determinants growth. Through machine learning-guided three-dimensional reconstruction, capture morphometric changes growing skin. We find that most growth driven by proliferation epidermis response mechanical tension, with more limited dermal subdermal...
According to the American Diabetes Association (ADA), 9-12 million patients suffer from chronic ulceration each year, costing healthcare system over USD $25 billion annually. There is a significant unmet need for new and efficacious therapies accelerate closure of non-healing wounds. Nitric Oxide (NO) levels typically increase rapidly after skin injury in inflammatory phase gradually diminish as wound healing progresses. The effect increased NO concentration on promoting re-epithelization...
Abstract Chronic wounds impose a significant healthcare burden to broad patient population. Cell-based therapies, while having shown benefits for the treatment of chronic wounds, have not yet achieved widespread adoption into clinical practice. We developed CRISPR/Cas9 approach precisely edit murine dendritic cells enhance their therapeutic potential healing wounds. Using single-cell RNA sequencing tolerogenic cells, we identified N-myc downregulated gene 2 ( Ndrg2 ), which marks specific...
Modulating mechanotransduction by inhibiting yes-associated protein (YAP) in mice yields wound regeneration without scarring. However, rodents are loose-skinned and fail to recapitulate key aspects of human repair. We sought elucidate the effects YAP inhibition red Duroc pig wounds, most human-like model show that one-time treatment with verteporfin, a inhibitor, immediately after wounding is sufficient prevent scarring drive pigs. By performing single-cell RNA sequencing (scRNA-seq) on...