A5065419895- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Bioinformatics and Genomic Networks
- Genomics and Chromatin Dynamics
- Cardiac Valve Diseases and Treatments
- Connective tissue disorders research
- Microbial Metabolic Engineering and Bioproduction
- Wound Healing and Treatments
- Wnt/β-catenin signaling in development and cancer
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- biodegradable polymer synthesis and properties
- Aortic Disease and Treatment Approaches
- RNA regulation and disease
- Mosquito-borne diseases and control
- Biofuel production and bioconversion
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Advanced Nanomaterials in Catalysis
- Neuroinflammation and Neurodegeneration Mechanisms
Stanford University
2025
Saarland University
2020-2024
Helmholtz Institute for Pharmaceutical Research Saarland
2024
Which genes, gene sets or pathways are regulated by certain miRNAs? miRNAs regulate a particular target pathway in physiological context? Answering such common research questions can be time consuming and labor intensive. Especially for researchers without computational experience, the integration of different data sources, selection right parameters concise visualization demanding. A comprehensive analysis should central to present adequate answers complex biological questions. With...
Abstract Small non-coding RNAs (sncRNAs) are pervasive regulators of physiological and pathological processes. We previously developed the human miRNA Tissue Atlas, detailing expression miRNAs across organs in body. Here, we present an updated resource containing sequencing data 188 tissue samples comprising 21 organ types retrieved from six humans. Sampling same bodies minimizes intra-individual variability facilitates making a precise high-resolution body map transcriptome. The allow...
Abstract Molecular mechanisms of organismal and cell aging remain incompletely understood. We, therefore, generated a body-wide map noncoding RNA (ncRNA) expression in (16 organs at ten timepoints from 1 to 27 months) rejuvenated mice. We found molecular trajectories are largely tissue-specific except for eight broadly deregulated microRNAs (miRNAs). Their individual abundance mirrors their presence circulating plasma extracellular vesicles (EVs) whereas ncRNAs were less present. For...
Neurodegenerative diseases and infections can produce lasting effects on brain function, yet the spatial molecular mechanisms underlying these changes remain poorly understood. Here, we present high-resolution transcriptomics of 40 postmortem samples from patients with Parkinson's disease, frontotemporal dementia, dementia Lewy bodies, severe COVID-19. Analyzing over 1.5 million spatially resolved cells across dorsolateral prefrontal cortex anterior cingulate revealed disease-specific...
An in-depth understanding of the molecular processes composing aging is crucial to develop therapeutic approaches that decrease as a key risk factor for cognitive decline. Herein, we present spatio-temporal brain atlas (15 different regions) microRNA (miRNA) expression across mouse lifespan (7 time points) and two interventions composed 1009 samples. MiRNAs are promising targets, they silence genes by complementary base-pair binding messenger RNAs known mediate speed. We first established...
Abstract The identification of targetomes remains a challenge given the pleiotropic effect miRNAs, limited effects miRNAs on individual targets, and sheer number estimated miRNA–target gene interactions (MTIs), which is around 44,571,700. Currently, targetome for single relies computational evidence functional studies covering smaller numbers targets. To ensure that analysis could be experimentally verified by assays, we employed systematic approach explored four (miR-129-5p, miR-129-1-3p,...
Previous work on murine models and humans demonstrated global as well tissue-specific molecular ageing trajectories of RNAs. Extracellular vesicles (EVs) are membrane mediating the horizontal transfer genetic information between different tissues. We sequenced small regulatory RNAs (sncRNAs) in two mouse plasma fractions at five time points across lifespan from 2–18 months: (1) sncRNAs that free-circulating (fc-RNA) (2) bound outside or inside EVs (EV-RNA). Different sncRNA classes exhibit...
Engineered P . putida H sets a benchmark in lignin‐based PHA. Balancing the catechol degrading pathways enables improved PHA production. A DO‐stat fed‐batch process permits efficient conversion of lignin hydrolysates into biomass and biopolymer.
Background: Turner Syndrome (TS) is a relatively rare X-chromosomal disease with increased cardiovascular morbidity and mortality. This study aimed to identify whether the circulating miR-126-3p/5p are involved in pathophysiology of vascular dysfunction TS. Methods: Using RT-qPCR, abundance levels miR-126-3p miR-126-5p were determined 33 TS patients age-matched healthy volunteers (HVs). Vascular screening, including assessment blood pressure, pulse wave velocity, augmentation index, aortic...
Little is known about abundance level changes of circulating microRNAs (miRNAs) and messenger RNAs (mRNA) in patients with Ebstein’s anomaly (EA). Here, we performed an integrated analysis to identify the differentially abundant miRNAs mRNA targets potential therapeutic that might be involved mechanisms underlying EA. A large panel human miRNA microarrays were conducted determine genome-wide expression profiles blood 16 EA age gender-matched healthy control volunteers (HVs). Differential...
RNA sequencing data sets rapidly increase in quantity. For microRNAs (miRNAs), frequently dozens to hundreds of billion reads are generated per study. The quantification annotated miRNAs and the prediction new leading computational tasks. Now, increased depth coverage allows gain deeper insights into variability miRNAs. analysis isoforms (isomiRs) is a trending topic, range tools for isomiRs has been developed. We provide an overview on 27 available solutions isomiRs. These include both...
Abstract Altered intercellular communication is one of the molecular hallmarks aging. Microvesicles carrying different kinds molecules, like proteins and RNAs are important agents short- long-range in body. MicroRNAs (miRNAs) especially interesting targets to study this context, as these molecules can regulate multiple recipient cells via complementary base-pair binding thereby a complex process such
Non-thermal plasma, a partially ionized gas, holds significant potential for clinical applications, including wound-healing support, oral therapies, and anti-tumour treatments. While its applications showed promising outcomes, the underlying molecular mechanisms remain incompletely understood. We thus apply non-thermal plasma to mouse auricular skin conducted non-coding RNA sequencing, as well single-cell blood sequencing. In time-series analysis (five timepoints spanning 2 hours), we...
Abstract Aging is a multifactorial and complex physiological process, affecting every organ with characteristic manifestations. Understanding the molecular mechanisms that drive aging processes crucial to targeting age-related disorders. Recent reports suggest severe post-infection syndromes can partially accelerate aging. However, underlying gene-encoded regulatory interplay, whether being shared or distinct between infection biology are poorly understood. Here, we employed spatial...
Patients with transposition of the great arteries (TGA) have different connected systemic chambers and this determines long-term morbidities survival. Limited findings been reported to systematically identify miRNA mRNA expression levels in such cohorts patients. In study, we aimed characterize miRNAs, mRNAs, miRNA-mRNA interaction networks patients TGA, a left (LV) right ventricle (RV).Large panel human microarrays were conducted determine genome-wide profiles blood 16 TGA-RV patients,...