A5067874269- MicroRNA in disease regulation
- Genetic Neurodegenerative Diseases
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Advanced biosensing and bioanalysis techniques
- Immune cells in cancer
- RNA modifications and cancer
- Phagocytosis and Immune Regulation
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- Diabetes and associated disorders
- Extracellular vesicles in disease
- Gene Regulatory Network Analysis
- Chemokine receptors and signaling
- Food Allergy and Anaphylaxis Research
- Neonatal Respiratory Health Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Nanomaterials in Catalysis
- Allergic Rhinitis and Sensitization
- Microplastics and Plastic Pollution
- Ion channel regulation and function
- Single-cell and spatial transcriptomics
- Adipokines, Inflammation, and Metabolic Diseases
Saarland University
1991-2025
Institute of Human Genetics
2021
Abstract MicroRNAs (miRNAs) play indispensable roles in posttranscriptional gene regulation. Their cellular regulatory impact is determined not solely by their sheer number, which likely amounts to >2000 individual miRNAs human, than the effectiveness of single miRNAs. Although, one begins develop an understanding complex mechanisms underlying miRNA–target interactions (MTIs), overall knowledge MTI functionality still rather patchy. In this critical review, we summarize key features...
<h3>Importance</h3> The overall low survival rate of patients with lung cancer calls for improved detection tools to enable better treatment options and patient outcomes. Multivariable molecular signatures, such as blood-borne microRNA (miRNA) may have high rates sensitivity specificity but require additional studies large cohorts standardized measurements confirm the generalizability miRNA signatures. <h3>Objective</h3> To investigate use miRNAs potential circulating markers detecting in an...
We present GeneTrail 3, a major extension of our web service that offers rich functionality for the identification, analysis, and visualization deregulated biological processes. Our provides comprehensive collection processes signaling pathways 12 model organisms can be analyzed with powerful framework enrichment network analysis transcriptomic, miRNomic, proteomic, genomic data sets. Moreover, novel workflows epigenetic marks, time series experiments, single cell data. demonstrate...
MicroRNAs are regulators of gene expression. A wide-spread, yet not validated, assumption is that the targetome miRNAs non-randomly distributed across transcriptome and targets share functional pathways. We developed a computational experimental strategy termed high-throughput miRNA interaction reporter assay (HiTmIR) to facilitate validation target First, pathways predicted prioritized by means increase specificity positive predictive value. Second, novel webtool miRTaH facilitates guided...
Background In 2016 the first-in-human phase I study of a miRNA-based cancer therapy with liposomal mimic microRNA-34a-5p (miR-34a-5p) was closed due to five immune related serious adverse events (SAEs) resulting in four patient deaths. For future applications miRNA mimics it is mandatory unravel effects both on tumor tissue and cells. Here, we set out analyze impact miR-34a-5p over-expression CXCL10/CXCL11/CXCR3 axis, which central for development an effective control. Methods We performed...
Although a pivotal role of microRNA (miRNA, miR) in the pathogenesis Huntington's disease (HD) is increasingly recognized, molecular functions miRNAs pathomechanisms HD await further elucidation. One that have been associated with miR-34a-5p, which was deregulated mouse R6/2 model and human brain tissues.The aim our study to demonstrate interactions between miR-34a-5p genes. By computational means we predicted 12 801 potential target genes miR-34a-5p. An in-silico pathway analysis revealed...
Abstract Chronic obstructive pulmonary disease (COPD) significantly increases the risk of developing cancer. Biomarker studies frequently follow a case-control set-up in which patients diagnosed with are compared to controls. Longitudinal cohort such as COPD-centered German COPD and SYstemic consequences-COmorbidities NETwork (COSYCONET) study provide patient biomaterial base for discovering predictive molecular markers. We asked whether microRNA (miRNA) profiles blood collected from prior...
Chronic obstructive pulmonary disease (COPD) is associated with an increased risk of death, reducing life expectancy on average between 5 and 7 years. The survival time after diagnosis, however, varies considerably as a result the heterogeneity COPD. Therefore, markers that predict individual COPD patients are great value. We analyzed baseline molecular profiles collected 54 months follow-up data cohort study “COPD SYstemic consequences-COmorbidities NETwork” (COSYCONET). Genome-wide...
Abstract The identification of targetomes remains a challenge given the pleiotropic effect miRNAs, limited effects miRNAs on individual targets, and sheer number estimated miRNA–target gene interactions (MTIs), which is around 44,571,700. Currently, targetome for single relies computational evidence functional studies covering smaller numbers targets. To ensure that analysis could be experimentally verified by assays, we employed systematic approach explored four (miR-129-5p, miR-129-1-3p,...
Abstract Adjusting intracellular calcium signaling is an important feature in the regulation of immune cell function and survival. Here we show that miR-34a-5p, a small non-coding RNA deregulated many common diseases, regulator store-operated Ca 2+ entry (SOCE) calcineurin signaling. Upon miR-34a-5p overexpression, observed both decreased depletion ER content influx through release-activated channels. Based on silico target prediction identified multiple genes within pathways are implicated...
T cells are central to the immune response against various pathogens and cancer cells. Complex networks of transcriptional post-transcriptional regulators, including microRNAs (miRNAs), coordinate cell activation process. Available miRNA datasets, however, do not sufficiently dissolve dynamic changes controlled upon activation. Here, we established a quantitative time-resolved expression pattern for entire miRNome over period 24 h human T-cell Based on our identified miRNAs specified common...
Neurodegenerative disorders such as Alzheimer’s disease (AD) and Parkinson’s (PD) are characterized by the accumulation of misfolded proteins in endoplasmic reticulum (ER) unfolded protein response (UPR). Modulating UPR is one major challenges to counteract development neurodegenerative other diseases with affected UPR. Here, we show that miR-34a-5p directly targets IRE1α branch UPR, including genes BIP, IRE1α, XBP1. Upon induction ER stress neuronal cells, overexpression impacts resulting...
Parkinson's disease (PD) emerges as a complex, multifactorial disease. While there is increasing evidence that dysregulated T cells play central role in PD pathogenesis, elucidation of the pathomechanical changes related signaling still its beginnings. We employed time-resolved RNA expression upon activation peripheral CD4+ to track and functionally relate on cellular representative cases patients at different stages PD. only few miRNAs showed time-course PD, we identified groups genes with...
Conventional toxicological methods and integrated transcriptomic analysis were used to comprehensively assess the potential health hazard of residual metal nanoparticles accumulated in body due poor solubility.
The effect of prostaglandin D2 (PGD2) on colonic ion transport was studied in the Ussing chamber. PGD2 (10(-6) M) decreased baseline short-circuit current (Isc) two preparations rat colon descendens, a mucosa-submucosa preparation with and mucosa without submucosal plexus. In both preparations, inhibited neuronally mediated secretory responses to electric field stimulation, sea anemone toxin ATX II, different cholinergic agents. Unidirectional flux measurements revealed that diminished...
Enhanced plasmid