A5016378595- Cytokine Signaling Pathways and Interactions
- Cervical Cancer and HPV Research
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- interferon and immune responses
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Virus-based gene therapy research
- Circular RNAs in diseases
- Cancer Research and Treatments
- Cancer Mechanisms and Therapy
- IL-33, ST2, and ILC Pathways
- RNA modifications and cancer
- RNA regulation and disease
- Immune cells in cancer
- Transgenic Plants and Applications
- Herpesvirus Infections and Treatments
- NF-κB Signaling Pathways
- Ovarian cancer diagnosis and treatment
- Viral Infectious Diseases and Gene Expression in Insects
- T-cell and Retrovirus Studies
- Mesenchymal stem cell research
- Chemokine receptors and signaling
- Wound Healing and Treatments
University of Augsburg
2025
Saarland University
2015-2025
Institute of Virology of the Slovak Academy of Sciences
2019
Institute of Human Virology
2019
Herpesvirus infection initiates a range of perturbations in the host cell, which remain poorly understood at level individual cells. Here, we quantify transcriptome single human primary fibroblasts during first hours lytic with HSV-1. By applying generalizable analysis scheme, define precise temporal order early viral gene expression and propose set-wise emergence genes. We identify cell genes pathways relevant for by combining three different computational approaches: pathway overdispersion...
Abstract Cervical cancer is a consequence of persistent infection with human papillomaviruses (HPV). Progression to malignancy linked an inflammatory microenvironment comprising T-helper-17 (Th17) cells, T-cell subset protumorigenic properties. Neoplastic cells express only low endogenous levels the Th17 chemoattractant CCL20, and therefore, it unclear how are recruited cervical tissue. In this study, we demonstrate that CCL20 was predominantly expressed in stroma squamous cell carcinomas...
// Susanne V. Schmidt 1 , Stefanie Seibert 2 Barbara Walch-Rückheim Benjamin Vicinus Eva-Maria Kamionka Jennifer Pahne-Zeppenfeld Erich-Franz Solomayer 3 Yoo-Jin Kim 4 Rainer M. Bohle Sigrun Smola 1, Center for Molecular Medicine Cologne and Institute of Virology, University Cologne, Germany Saarland University, Homburg/Saar, Department Gynecology Obstetrics, Pathology, Correspondence to: Smola, e-mail: sigrun.smola@uks.eu Keywords: cervical cancer, human papilloma virus, dendritic...
Cervical carcinogenesis is a consequence of persistent infection with high-risk human papillomaviruses (HPVs). Recent studies indicate that HPV-transformed cells actively instruct their microenvironment to promote carcinogenesis. Here, we demonstrate cervical cancer activate monocytes produce own CCL2 for further monocyte recruitment and reprogram function during differentiation maturation dendritic (DCs). Our data show suppress the induction chemokine receptor CCR7 in phenotypically mature...
Abstract NF-κB functions as modulator of T cell receptor-mediated signaling and transcriptional regulator miR-34a. Our in silico analysis revealed that miR-34a impacts the signalosome with binding sites 14 key members pathway. Functional identified five target genes including PLCG1 , CD3E PIK3CB TAB2 NFΚBIA . Overexpression CD4 + CD8 cells led to a significant decrease most downstream cytoplasmic member, reduced surface abundance TCRA CD3E, reduction killing capacity. Inhibition caused an...
Infection with genus beta human papillomaviruses (HPV) is implicated in the development of non-melanoma skin cancer. This was first evidenced for HPV5 and 8 patients epidermodysplasia verruciformis (EV), a genetic disease. So far, it has been unknown how these viruses overcome cutaneous immune control allowing their persistence lesional epidermis patients. Here we demonstrate that Langerhans cells, essential immunosurveillance, are strongly reduced HPV8-positive from EV Interestingly, same...
Abstract Persistent infection with high-risk human papillomavirus (HPV) is a prerequisite for the development of cervical cancer. HPV-transformed cells actively instruct their microenvironment, promoting chronic inflammation and cancer progression. We previously demonstrated that contribute to Th17 cell recruitment, type protumorigenic properties. In this study, we analyzed expression Th17-promoting cytokine IL23 in micromilieu found CD83+ mature dendritic (mDC) coexpressing stroma squamous...
Micro(mi)RNAs are increasingly recognized as central regulators of immune cell function. While it has been predicted that miRNAs have multiple targets, the majority these predictions still await experimental confirmation. Here, miR-34a, a well-known tumor suppressor, is analyzed for targeting genes involved in system processes leucocytes. Using an in-silico approach, we combined miRNA target prediction with GeneTrail2, web tool Multi-omics enrichment analysis, to identify miR-34a genes,...
Abstract Background Monitoring individual therapy responses of patients with cancer represents a major clinical challenge providing the basis to early identify metastases and relapse. We previously demonstrated that radio- or chemoradiotherapy affects systemic cellular milieu vulvar cervical creates post-therapeutic environments associated Circulating tumor cells (CTCs) in are related relapse; however, their quantitative phenotypic characteristics during still unknown. Methods In this...
Abstract Neoadjuvant radio/chemotherapy regimens can markedly improve cervical cancer outcome in a subset of patients, while other patients show poor responses, but may encounter severe adverse effects. Thus, there is strong need for predictive biomarkers to clinical management patients. STAT3 considered as critical antiapoptotic factor various malignancies. We therefore investigated activation during carcinogenesis and its impact on the response cells chemotherapeutic drugs....
Abstract Adjusting intracellular calcium signaling is an important feature in the regulation of immune cell function and survival. Here we show that miR-34a-5p, a small non-coding RNA deregulated many common diseases, regulator store-operated Ca 2+ entry (SOCE) calcineurin signaling. Upon miR-34a-5p overexpression, observed both decreased depletion ER content influx through release-activated channels. Based on silico target prediction identified multiple genes within pathways are implicated...
T cells are central to the immune response against various pathogens and cancer cells. Complex networks of transcriptional post-transcriptional regulators, including microRNAs (miRNAs), coordinate cell activation process. Available miRNA datasets, however, do not sufficiently dissolve dynamic changes controlled upon activation. Here, we established a quantitative time-resolved expression pattern for entire miRNome over period 24 h human T-cell Based on our identified miRNAs specified common...
Parkinson's disease (PD) emerges as a complex, multifactorial disease. While there is increasing evidence that dysregulated T cells play central role in PD pathogenesis, elucidation of the pathomechanical changes related signaling still its beginnings. We employed time-resolved RNA expression upon activation peripheral CD4+ to track and functionally relate on cellular representative cases patients at different stages PD. only few miRNAs showed time-course PD, we identified groups genes with...
Cervical cancer therapy is still a major clinical challenge, as patients substantially differ in their response to standard treatments, including chemoradiotherapy (CRT). During cervical carcinogenesis, T‐helper (Th)‐17 cells accumulate the peripheral blood and tumor tissues of are associated with poor prognosis. In this prospective study, we find increased Th17 frequencies after post‐therapeutic ratio Th17/CD4 + T > 8% was early recurrence. Furthermore, promote resistance toward CRT,...
Persistent genus β-HPV (human papillomavirus) infection is a major co-factor for non-melanoma skin cancer in patients suffering from the inherited disease epidermodysplasia verruciformis (EV). Malignant EV lesions are particularly associated with HPV type 5 or 8. There clinical and molecular evidence that HPV8 actively suppresses epithelial immunosurveillance by interfering recruitment of Langerhans cells, which may favor viral persistence. Mechanisms how persistent promotes carcinogenic...
For vulvar cancers, radiotherapy is targeting cancer cells, but also affects the host immune system. As this may affect treatment outcome, in prospective study, we characterized individual T cell milieu induced by surgery and adjuvant radio +/- chemotherapy (aRT) systemically blood of patients found increased frequencies Interleukin (IL)-17-producing CD4+ CD8+ cells after aRT while Th1 perforin-producing killer were strongly diminished. Phenotypic characterization revealed enhanced...
Non-thermal plasma, a partially ionized gas, holds significant potential for clinical applications, including wound-healing support, oral therapies, and anti-tumour treatments. While its applications showed promising outcomes, the underlying molecular mechanisms remain incompletely understood. We thus apply non-thermal plasma to mouse auricular skin conducted non-coding RNA sequencing, as well single-cell blood sequencing. In time-series analysis (five timepoints spanning 2 hours), we...
Abstract Herpesvirus infection initiates a range of perturbations in the host cell, which remain poorly understood at level individual cells. Here, we quantified transcrips single human primary fibroblasts during first hours lytic with HSV-1. By applying generalizable analysis scheme, defined precise temporal order early viral gene expression and found unexpected bifurcations bottlenecks. We identified cell genes pathways relevant by combining three different computational approaches:...
<div>Abstract<p>Neoadjuvant radio/chemotherapy regimens can markedly improve cervical cancer outcome in a subset of patients, while other patients show poor responses, but may encounter severe adverse effects. Thus, there is strong need for predictive biomarkers to clinical management patients. STAT3 considered as critical antiapoptotic factor various malignancies. We therefore investigated activation during carcinogenesis and its impact on the response cells chemotherapeutic...
<p>(A) NECF were stimulated with 50 µg/ml PMA or medium for 24h. Whole cell extracts analyzed C/EBPβ expression by Western blot. Shown is one experiment out of n=3, quantification represents n=3. (B) (lower panel) (upper 24h and co-stained DAPI (blue), anti-Vimentin (red) anti-C/EBPβ (green) in Immunofluorescence. (C) Supernatants collected CCL20 protein expression. are the values{plus minus}SD averaged from n=3 experiments performed triplicates.</p>