A5021662708- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Wound Healing and Treatments
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer Cells and Metastasis
- Pressure Ulcer Prevention and Management
- RNA Interference and Gene Delivery
- Bone fractures and treatments
- Curcumin's Biomedical Applications
- Body Contouring and Surgery
- Angiogenesis and VEGF in Cancer
- Biomedical Ethics and Regulation
- Muscle Physiology and Disorders
- Synthesis and Characterization of Heterocyclic Compounds
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- Immune cells in cancer
- Osteoarthritis Treatment and Mechanisms
- Cell death mechanisms and regulation
- Tendon Structure and Treatment
- Pluripotent Stem Cells Research
- Immunotherapy and Immune Responses
University of New Orleans
2017-2025
Tulane University
2011-2022
LaCell (United States)
2017-2020
Louisiana State University
2018
Dillard University
2018
Center for Neuroscience and Regenerative Medicine
2017
Louisiana State University System
2013
Pennington Biomedical Research Center
2013
Baton Rouge Clinic
2013
Background Fat grafting is used to restore breast defects after surgical resection of tumors. Supplementing fat grafts with adipose tissue-derived stromal/stem cells (ASCs) proposed improve the regenerative/restorative ability graft and retention. However, long term safety for ASC in proximity residual cancer unknown. The objective this study was determine impact human ASCs derived from abdominal lipoaspirates three donors, on a model that exhibits early metastasis. Methodology/Principal...
Abstract Background Obesity is associated with a higher risk of developing cancer and co-morbidities that are part the metabolic syndrome. Adipose tissue recognized as an endocrine organ, it affects number physiological functions, contains adipose tissue-derived stem cells (ASCs). ASCs can differentiate into multiple lineages, such applicable to engineering regenerative medicine. Yet question whether ASC functionality affected by donor’s body mass index (BMI) still exists. Results were...
Abstract More than 2.5 million patients in the U.S. require treatment for pressure ulcers annually, and elderly are at particularly high risk ulcer development. Current therapy consists of conservative medical management shallow lesions aggressive debridement surgery deeper lesions. The current study uses a murine model to address hypothesis that adipose-derived stromal/stem cell (ASC) would accelerate enhance repair. dorsal skin both young (2 months old [mo]) (20 mo) C57BL/6J female mice...
Monitoring wound progression over time is a critical aspect for studies focused on in-depth molecular analysis or evaluating the efficacy of potential novel therapies. Histopathological biopsies can provide significant insight into healing dynamics, yet there no standardized and reproducible scoring system currently available. The purpose this study was to develop statistically validate based parameters in each phase that be easily accurately assessed using either Hematoxylin & Eosin (H&E)...
Tissue engineering is a promising approach for the repair and regeneration of cartilaginous tissue. Appropriate three-dimensional scaffolding materials that mimic cartilage are ideal chondral defects. The emerging decellularized tissue-based scaffolds have potential to provide essential biochemical signals structural integrity, which mimics natural tissue environment directs cellular fate. Umbilical cord-derived hydrogels function as 3D material, support adherence, proliferation, migration,...
Adipose derived stromal/stem cells (ASCs) hold potential as cell therapeutics for a wide range of disease states; however, many expansion protocols rely on the use fetal bovine serum (FBS) culture nutrient supplement. The current study explores substitution lysates from expired human platelets (HPLs) an FBS substitute.Expired authorized blood center were lysed by freeze/thawing and used to examine ASCs with respect proliferation using hematocytometer counts, colony forming unit-fibroblast...
Decellularized human adipose tissue has potential clinical utility as a processed biological scaffold for soft cosmesis, grafting, and reconstruction. Adipose decellularization been accomplished using enzymatic-, detergent-, and/or solvent-based methods. To examine the hypothesis that distinct processes may yield scaffolds with differing compositions, current study employed mass spectrometry to compare proteomes of adipose-derived matrices generated through three independent methods...
White adipose tissue (WAT) is a critical organ in both health and disease. However, physiologically faithful culture models of primary human WAT remain limited, at best. In this study we describe novel system which sandwiched between tissue-engineered sheets adipose-derived stromal cells. This construct, called "sandwiched white tissue" (SWAT), can be defined as microphysiological (MPS) since it tissue-engineered, multicellular, three-dimensional construct produced using We validated SWAT...
Background: Adipose tissue is a source of adipose-derived stromal/stem cells for engineering and reconstruction fat grafts. Although liposuction simple procedure the harvest adipose tissue, repetition this surgical intervention can cause adverse effects to patient be limiting factor immediate use. Cryopreservation avoid morbidity associated with repetitive liposuction, allowing use stored after initial procedure. This article focuses on characterization fresh cryopreserved human tissue....
Obesity, defined as a body mass index of 30 kg/m2 or above, has increased considerably in incidence and frequency within the United States globally. Associated comorbidities including cardiovascular disease, type 2 diabetes mellitus, metabolic syndrome, nonalcoholic fatty liver disease have led to focus on mechanisms promoting prevention treatment obesity. Commonly utilized vitro models employ human mouse preadipocyte cell lines 2-dimensional (2D) format. Due structural, biochemical,...
As the world's population lives longer, number of individuals at risk for pressure ulcers will increase considerably in coming decades. In developed countries, up to 18% nursing home residents suffer from and resulting hospital costs can account 4% a nation's health care budget. Although full-thickness surgical skin wounds have been used as model, preclinical rodent studies demonstrated that repeated cycles ischemia reperfusion created by exposure magnets most closely mimic human ulcer...
Progenitors derived from the stromal vascular fraction (SVF) of white adipose tissue (WAT) possess ability to form clonal populations and differentiate along multiple lineage pathways. However, literature continues vacillate between defining adipocyte progenitors as "stromal" or "stem" cells. Recent studies have demonstrated that a nonpericytic subpopulation cells, which phenotype, CD45(-) /CD31(-) /CD146(-) /CD34(+) , are mesenchymal, suggest this may be an endogenous progenitor within...
Adipose tissue is now recognized as a functional organ that contains cellular heterogeneity and diversity within anatomical depots. The stromal vascular fraction (SVF) of adipose endothelial progenitors, fibroblasts, lymphocytes, monocyte/macrophages, pericytes, pre-adipocytes, stromal/stem cells, among others. In recent years, there has been growing appreciation the influence age gender in field stem cell biology. Yet few studies have evaluated biological or sex on either SVF...