Akt is a major cell survival and angiogenic mediator downstream of angiopoietin-1 (Ang-1)/Tie-2 signaling pathway. We hypothesize that transplantation mesenchymal stem cells (MSCs) co-overexpressing Ang-1 lead to better prognosis. genes were adenovirally transduced into MSCs from male Fischer rats. Cytoprotective effects transgene overexpression in vitro assessed by exposure 8 hours anoxia. TUNEL measurement lactate dehydrogenase showed (MAAs) more resistant anoxia as compared with the...

Tissue engineering is a promising approach for the repair and regeneration of cartilaginous tissue. Appropriate three-dimensional scaffolding materials that mimic cartilage are ideal chondral defects. The emerging decellularized tissue-based scaffolds have potential to provide essential biochemical signals structural integrity, which mimics natural tissue environment directs cellular fate. Umbilical cord-derived hydrogels function as 3D material, support adherence, proliferation, migration,...

Abstract Extracellular matrix‐based bio‐scaffolds are useful for tissue engineering as they retain the unique structural, mechanical, and physiological microenvironment of thus facilitating cellular attachment matrix activities. However, considering its potential, a comprehensive understanding protein profile remains elusive. Herein, we evaluate impact decellularization on human amniotic membrane (hAM) based proteome profile, physicochemical features, well attachment, viability,...

Introduction: Ex vivo preconditioning increases the therapeutic potential of mesenchymal stem cells (MSCs) in terms antioxidant activity, growth factor production, homing, differentiation, and immunomodulation. Therefore, it is considered an effective strategy to be used before transplantation application MSCs. Histone deacetylase inhibitor (HDACi), valproic acid (VPA), has been reported induce hepatic differentiation Although individual studies have shown that epigenetic modification...

Cold burn wounds differ in their pathophysiological spectrum as compared to other types of wounds. These have prolonged devastating effects on the body including hypertrophic scars, contracture, and necrosis. Mesenchymal stem cells (MSCs) are considered promising candidates for complete regeneration However, transplanted MSCs face challenge survive under harsh tissue conditions. Preconditioning with bioactive compounds may enhance survival regenerative potential use clinical applications....

Mesenchymal stem cells (MSCs) have immense self-renewal capability. They can be differentiated into many cell types and therefore hold great potential in the field of regenerative medicine. MSCs converted beating cardiomyocytes by treating them with DNA-demethylating agents. Some these compounds are nucleoside analogs that widely used for studying role DNA methylation biological processes as well clinical treatment leukemia other carcinomas.To achieve a better therapeutic option...

Umbilical cord-derived mesenchymal stem cells preconditioned with isorhamnetin: potential therapy for burn wounds

Intervertebral disc degeneration (IVDD) is the leading cause of lower back pain. Disc characterized by reduced cellularity and decreased production extracellular matrix (ECM). Mesenchymal stem cells (MSCs) have been envisioned as a promising treatment for degenerative illnesses. Cell-based therapy using ECM-producing chondrogenic derivatives MSCs has potential to restore functionality intervertebral (IVD).To investigate transcription factors promote differentiation human umbilical cord into...

Rap1, a member of Ras superfamily small GTP-binding proteins, is involved in cardiovascular biology numerous ways. It an evolutionary conserved regulator adhesion, polarity, differentiation and growth.Our aim was to analyze Rap1-activated rat bone marrow mesenchymal stem cells (MSCs) for their potential role adhesion cardiac differentiation.Myocardial infarction (MI) produced Sprague Dawley (SD) rats through occlusion the left anterior descending coronary artery. MSCs were treated with...

Intervertebral disc (IVD) shows aging and degenerative changes earlier than any other body connective tissue. Its repair regeneration provide a considerable challenge in regenerative medicine due to its high degree of infrastructure mechanical complexity. Mesenchymal stem cells, their tissue resurfacing potential, represent many explanatory pathways regenerate breakdown. This study was undertaken evaluate the co-regulation Sox9 TGFβ1 differentiating human umbilical cord mesenchymal cells...

Zinc (Zn) is the second most abundant trace element after Fe, present in human body. It frequently reported association with cell growth and proliferation, its deficiency considered to be a major disease contributing factor.To determine effect of Zn on vitro proliferation umbilical cord (hUC)-derived mesenchymal stem cells (MSCs).hUC-MSCs were isolated from tissue characterized based immunocytochemistry, immunophenotyping, tri-lineage differentiation. The impact cytotoxicity was determined...

Abstract The underlying pathophysiology of nonhealing chronic wounds is poorly understood due to the changes occurring at gene level and complexity arising in their proteomic profile. Here, we elucidated temporal differential profile normal diabetic wound‐healing mediators along with interactions associated pathways. Skin tissues corresponding were isolated Days 0, 3, 6, 9 representing different healing phases. Temporal expression was analyzed by quantitative real‐time PCR. Concurrently,...

Hypoxic wounds are tough to heal and associated with chronicity, causing major healthcare burden. Available treatment options offer only limited success for accelerated scarless healing. Traditional skin substitutes widely used improve wound healing, however, they lack proper vascularization. Mesenchymal stem cells (MSCs) improved healing; their poor retention, survival adherence at the site negatively affect therapeutic potential. The aim of this study is enhance regeneration in a rat model...

Owing to high proliferation rate, multipotency and self-renewal capability, dental pulp stem cells (DPSC) from human exfoliated teeth (SHED) have become cell source of choice for based regenerative therapies. We aimed compare DPSC SHED as sources with a future use in regeneration calcified tissue.Explant derived (n=9) (n=1) were cryopreserved, thawed expanded analysis population doubling time, colony forming unit assay efficiency. A growth curve was plotted determine while numbers efficiency...

Background: Oxidative stress has been reported to contribute aging and cataract formation in the lens. The aim was determine association of oxidative with advanced glycation end products (AGEs) elderly diabetic non-diabetic patients cataract. Methods: In present study, malondialdehyde, vitamin E, serum AGEs, glycemic control were investigated. study included 156 subjects. Out them, 30 normal subjects, 31 without cataract, 33 32 young selected on clinical grounds from Eye Ward, Jinnah...

Mesenchymal stem cells (MSCs) hold significant promise as potential therapeutic candidates following cardiac injury. However, to ensure survival of transplanted in ischemic environment, it is beneficial precondition them with growth factors that play important role cell and proliferation. Aim this study use interleukin-7 (IL-7), a factor, enhance the rat bone marrow MSCs terms fusion vitro function vivo.Mesenchymal were transfected IL-7 gene through retroviral vector. Normal co-cultured...