A5034073367- Wound Healing and Treatments
- Skin Protection and Aging
- Mesenchymal stem cell research
- Dermatologic Treatments and Research
- Hair Growth and Disorders
- Tendon Structure and Treatment
- Redox biology and oxidative stress
- Cancer, Hypoxia, and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Electrospun Nanofibers in Biomedical Applications
- Skin and Cellular Biology Research
- Heat shock proteins research
- Reproductive Biology and Fertility
- Genomics, phytochemicals, and oxidative stress
- Tissue Engineering and Regenerative Medicine
- Hippo pathway signaling and YAP/TAZ
- Adipokines, Inflammation, and Metabolic Diseases
- Hormonal and reproductive studies
- Diabetic Foot Ulcer Assessment and Management
- Nail Diseases and Treatments
- Sperm and Testicular Function
Institute of Animal Reproduction and Food Research
2020-2024
Polish Academy of Sciences
2020-2024
Monitoring wound progression over time is a critical aspect for studies focused on in-depth molecular analysis or evaluating the efficacy of potential novel therapies. Histopathological biopsies can provide significant insight into healing dynamics, yet there no standardized and reproducible scoring system currently available. The purpose this study was to develop statistically validate based parameters in each phase that be easily accurately assessed using either Hematoxylin & Eosin (H&E)...
Since there are limited studies analyzing the impact of age, sex and obesity on cutaneous repair, current study evaluated excisional skin wound healing as a function diet in C57BL/6 mice subjected to either low (LFD) or high (HFD) fat diet.Older accumulated increased body relative younger under HFD.Skin at particular stages was affected by age aspect Tgfβ-1, MCP-1, Mmp-9 Mmp-13 expression.The most profound, cumulative effect observed for combination two parameters: sex.While males displayed...
Dermal white adipose tissue (dWAT) is involved in the maintenance of skin homeostasis. However, studies concerning its molecular regulation are limited. In present paper, we ask whether introduction two transcription factors, Foxn1 and Hif-1α, into post-wounded Foxn1-/- mice regulates dWAT during wound healing (days 3 6). We have chosen lentivirus vectors (LVs) as a tool to deliver Hif-1α skin. documented that combinations both transgenes reduces number, size diameter dermal adipocytes at...
Skin exposed to environmental threats, including injuries and oxidative stress, develops an efficient but not fully recognized system of repair antioxidant protection. Here, using mass spectrometry analysis (LC–MS/MS), followed by in vitro vivo experiments, we provided evidence that Foxn1 keratinocytes regulates elements the electron transport chain participates thioredoxin (Txn2, Txnrd3, Srxn1) induction, particularly a hypoxic environment. We first showed upregulates glutathione reductase...
The primary mechanism by which adipose-derived stem cells (ASCs) exert their reparative or regenerative potential relies predominantly on paracrine action. Secretory abilities of ASCs have been found to be amplified hypoxia pre-conditioning. This study investigates the impact (1% O2) secretome composition pig (pASCs) and explores effect pASCs’ conditioned media (CM) skin cell functions in vitro expression markers attributed wound healing. Exposure pASCs increased levels vascular endothelial...
Hypoxia and hypoxia-regulated factors (eg, hypoxia-inducible factor-1α [Hif-1α], factor inhibiting Hif-1α [Fih-1], thioredoxin-1 [Trx-1], aryl hydrocarbon receptor nuclear translocator 2 [Arnt-2]) have essential roles in skin wound healing. Using Foxn1−/− mice that can heal injuries a unique scarless manner, we investigated the interaction between Foxn1 factors. The displayed impairments regulation of Hif-1α, Trx-1, Fih-1 but not Arnt-2 during healing process. An analysis wounded showed...
The recognition of a distinct fat depot, the dermal white adipose tissue (dWAT), points out complexity interaction among skin resident cells: keratinocytes, fibroblasts (DFs) and adipocytes in response to physiological (diet, age) pathological (injury) stimulations. dWAT has been recognized as significant contributor thermoregulation, hair cycle, immune response, wound healing scarring. In this study, we examined age- diet-related changes modulation DFs’ adipogenic potential. data showed...
Adipose-derived stem cells (ASCs) from human and animal fat have emerged as therapeutic alternatives for damaged tissues. Pre-conditioning of ASCs with hypoxia results in their functional enhancement, which might facilitate the process healing. However, there is still a critical need large-scale preclinical studies to reinforce translation these findings into clinical practice humans veterinary medicine. Here, we adapted full-thickness excisional skin wound mouse model evaluate compare...
Abstract Intradermal adipocytes form dermal white adipose tissue (dWAT), a unique fat depot localized in the lower layer of dermis. However, recognition molecular factors regulating dWAT development, homeostasis, and bioactivity is limited. Using Foxn1 −/− +/+ mice, we demonstrated that epidermally expressed regulates development defines adipogenic capacity fibroblasts. In intact post‐wounded skin, contributes to initial stimulation adipogenesis participates modulation lipid metabolism...
Although the impact of age, gender, and obesity on skin wound healing process has been extensively studied, data related to gender differences in aspects scarring are limited. The present study performed abdominal human intact scar focused determining extracellular matrix (ECM) composition, dermal white adipose tissue (dWAT) accumulation, Foxn1 expression as a part response injury. Scar men showed highly increased levels COLLAGEN 1A1, 6A3, ELASTIN mRNA expression, accumulation thick collagen...
ABSTRACT Hypoxia and hypoxia-regulated factors [e. g., hypoxia-inducible factor-1α (Hif-1α), factor inhibiting Hif-1α (Fih-1), thioredoxin-1 (Trx-1), aryl hydrocarbon receptor nuclear translocator 2 (Arnt-2)] have essential roles in skin wound healing. Using Foxn1 −/− mice that can heal injuries a unique scarless manner, we investigated the interaction between factors. The displayed impairments regulation of Hif-1α, Trx-1 Fih-1 but not Arnt-2 during healing process. An analysis wounded...