A5046084314- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Meat and Animal Product Quality
- Animal Genetics and Reproduction
- Probiotics and Fermented Foods
- Fish Ecology and Management Studies
- Aquaculture Nutrition and Growth
- Proteins in Food Systems
- Seed Germination and Physiology
- Milk Quality and Mastitis in Dairy Cows
- Marine Bivalve and Aquaculture Studies
- Bee Products Chemical Analysis
- Infant Nutrition and Health
- Physiological and biochemical adaptations
- Fermentation and Sensory Analysis
- Rabbits: Nutrition, Reproduction, Health
- Insect and Arachnid Ecology and Behavior
- Phytase and its Applications
- Environmental Toxicology and Ecotoxicology
- Horticultural and Viticultural Research
- Food composition and properties
- Reproductive Health and Technologies
- Hormonal and reproductive studies
University of Molise
2019-2025
University of Foggia
2016-2017
Abstract This study was designed to optimize the semen freezing protocol of native Mediterranean brown trout inhabiting Molise rivers through two experiments: an in vitro analysis effects basic extenders combined with three cryoprotectants on post-thaw quality; and vivo test assess fertilization hatching rate. Semen diluted at a ratio 1:3 medium composed glucose extender (A) or mineral (B). Each component contained 10% dimethylsulfoxide, dimethylacetamide methanol. The post-semen quality...
Over the years, efforts have been made to establish a reference protocol for first Italian rabbit semen bank. Here, we will provide an overview of our group’s key discoveries, which led us identify effective freezing semen. However, drawback involves diluting with media at fixed dilution ratios, resulting in significant variability sperm number per straw. Addressing this limitation underscores need standardise by investigating effect concentrations within straws on reproductive performances...
Semen cryopreservation represents the main tool for preservation of biodiversity; however, in avian species, freezing–thawing process results a sharp reduction sperm quality and consequently fertility. Thus, to gain first insight into molecular basis turkey sperm, NMR-assessed metabolite profiles fresh frozen–thawed samples were herein investigated compared with qualitative parameters. Cryopreservation decreased viability, mobility, osmotic tolerance samples. This decrease was associated...
The Mediterranean brown trout is one of the most endangered freshwater species. A complicated network climate and human influences has severely harmed its biodiversity. introduction alien serious threats to native populations’ intraspecific diversity. In Molise region (south-Italy) an important conservation program (LIFE Nat.Sal.Mo project) recently been proposed preserve genetic integrity trout. This project, alongside safeguarding re-establishing habitats’ usefulness aims restore...
The aim of our study was to test the effects different non-permeating cryoprotectants (NP-CPAs), namely low-density lipoproteins (LDLs), sucrose, and egg yolk, thawing rates on post-thaw semen quality fertilizing ability native Mediterranean brown trout. Pooled samples were diluted 1:3 (v:v) with 2.5%, 5%, 10%, or 15% LDL; 0.05, 0.1, 0.3 M sucrose; 10% yolk. At moment analysis, thawed at 30 °C/10 s 10 °C/30 s. evaluated, considering motility, duration viability, DNA integrity. Significantly...
The present study aimed to find an effective cryopreservation protocol for turkey semen through the combined use of dimethylsulfoxide (DMSO) and three non-permeant cryoprotectants (NP-CPAs), sucrose, trehalose, Ficoll 70. In addition, action two dilution rates (1:2 1:4) were also investigated. Semen was processed according final following treatments: Tselutin extender (TE)/DMSO (control), TE/DMSO + sucrose or trehalose 50, 100, 200, 400 mM, 0.5, 0.75, 1, 1.5 mM. total 26 different...
This study was designed to test the fertilizing ability of cryopreserved turkey semen, and here, two experiments were performed: an in vitro analysis assess effects Tselutin Lake diluents vivo determine fertility hatching rates by also studying feat three insemination doses (250, 400 600 × 106 sperm/hen). Pooled semen samples diluted with or extender which contained 20% dimethylsulfoxide 1 mM Ficoll at final sperm concentration 3 109 sperm/mL. Thereafter, packaged into straws frozen on...
The creation of genetic resource cryobanks provides a crucial link between in situ and ex techniques to improve the efficiency conservation programs. Aim present review is describe all activities developed for implementation first Italian Semen Cryobank Autochthonous Chicken Turkey Breeds. These can be classified into three main topics: (1) identification species-specific semen freezing/thawing reference procedures; (2) drafting Standard Operative Procedures (SOP) cryobank; (3) storage doses...
This study aimed to investigate the impact of sperm concentrations on in vitro quality cryopreserved rabbit semen. The semen pools (n = 8, from 80 donors) were split into five aliquots with final 15, 25, 35, 55, and 75 × 106 per straw. motility parameters (CASA system) membrane integrity (flow cytometric analysis) both evaluated at various stages cryopreservation process: fresh dilution, cooling, equilibration, immediately after 30 min post-thawing. results indicated significant influence...
The aim of our study was to test the effectiveness a simple semen cryopreservation procedure, developed for cultivated salmonid, on wild salmonid Mediterranean area and evaluate effect different thawing rates sperm-to-egg ratios. five individual males diluted into final extender concentration 0.15 M glucose 7.5% methanol loaded 0.25 mL plastic straws, sperm 3.0 × 109 sperm/mL obtained. After equilibration, straws were frozen by exposure liquid nitrogen vapor at 3 cm above level 5 min. thawed...
D-532 fertilization solution is generally used to replace the water or ovarian fluid during artificial reproductive practices in salmonids due its ability boost sperm motility and increase rates compared with natural activation media. However, maintenance of a microenvironment gives it advantage protecting eggs from potential harmful factors external environment simplifying field operations related removal when alone. In light this, aim present study was investigate vitro, for first time,...
Abstract This study has been designed to optimize the semen freezing protocol in rabbits, this regard we compared a Tris-citrate-glucose (TCG) extender with commercial one (Cortalap ® ), that best of our knowledge never used up now on vitro freezability and fertilizing ability cryopreserved rabbit semen. Two different inseminating doses were considered. Five pooled samples divided into two subsamples each them diluted ratio 1:1 (v:v) composed TCG or Cortalap containing 16% dimethylsulfoxide...
Significant changes in the quality and metabolic profile of fresh turkey sperm as a result both cryopreservation reproductive age have already been individually confirmed our previous studies. This new dataset adds relevant piece to tangled puzzle metabolite levels affecting cryopreserved quality, taking into consideration two different period ages. Fresh semen samples were collected at 44 56 weeks exposed process. All frozen-thawed subjected analysis mobility, viability osmotic tolerance...
This study provides a comparative assessment of fresh semen quality and sensitivity to cryopreservation in several Italian chicken breeds. The research involved 145 roosters from 13 results showed wide variability all the parameters considered among different breeds, especially quantitative variables semen, such as volume concentration. For qualitative characteristics (sperm membrane integrity motility parameters), across breeds was more pronounced for frozen than semen. Interestingly, apart...
Silter is an Italian hard cheese manufactured with milk produced by cows fed at different altitude, valley or alpine pasture.The chemical, rheological and sensory properties of cheeses can be affected the modification in composition due to breed which altitude causes protein content, κ-CN glycosylation, plasmin activity, coagulation properties.The influence activity on dairy production was investigated seven cheeses, four three from mountain, through alkaline urea-polyacrylamide gel...
Abstract This study aimed to improve postharvest management of flat oysters reared in a longline system the mid Adriatic Sea, using short‐term storage and package an innovative closed‐circuit system. For trial, 870 were employed, divided into three experimental groups (A, B, C), N = 270 each group, whereas remaining 60 used for 2 controls. Each group differed relation time spent depuration tank packaging: A was packed immediately transferred cell; B depurated 48 h, then C h packed, another...
The aim of this study was to evaluate the effects different cold-storage time intervals between collection and semen-freezing on both fresh cryopreserved semen motility parameters post-thaw fertilizing ability Mediterranean brown trout semen. ejaculates were split into six aliquots stored ice from 1 6 h, until freezing. Fresh sperm evaluated by a Computer-Assisted Sperm Analysis system, whilst assessed in vivo trials. In semen, at 3 h storage, significant decrease total motility, linear...
Sperm freezing is of interest not only for animal breeding. The ability to use sperm in frozen form AI a key factor ensuring the long-term preservation genetic diversity through creation cryobank. most widely used method involves using straws as packaging sperm, then them on liquid nitrogen (N2) vapor and finally immersing N2. cryosurvival cells also varies among different species has been correlated with rate, i.e., distance above N2 level. Identifying procedure that will report...