A5032854453- Wound Healing and Treatments
- Skin Protection and Aging
- Mesenchymal stem cell research
- Dermatologic Treatments and Research
- Electrospun Nanofibers in Biomedical Applications
- Hair Growth and Disorders
- Tissue Engineering and Regenerative Medicine
- Pressure Ulcer Prevention and Management
- Diabetic Foot Ulcer Assessment and Management
- Heme Oxygenase-1 and Carbon Monoxide
- Tendon Structure and Treatment
- Cancer, Hypoxia, and Metabolism
- Skin and Cellular Biology Research
- Wnt/β-catenin signaling in development and cancer
- Hippo pathway signaling and YAP/TAZ
- Adipokines, Inflammation, and Metabolic Diseases
- Nail Diseases and Treatments
- Heat shock proteins research
- Redox biology and oxidative stress
- melanin and skin pigmentation
Polish Academy of Sciences
2018-2024
Institute of Animal Reproduction and Food Research
2018-2024
Monitoring wound progression over time is a critical aspect for studies focused on in-depth molecular analysis or evaluating the efficacy of potential novel therapies. Histopathological biopsies can provide significant insight into healing dynamics, yet there no standardized and reproducible scoring system currently available. The purpose this study was to develop statistically validate based parameters in each phase that be easily accurately assessed using either Hematoxylin & Eosin (H&E)...
Skin injuries in mammals are healed through repair or regeneration. Our previous studies demonstrated that deficient expression of the transcription factor Foxn1 epidermis nude mice accounts for their skin's pronounced regenerative properties. Since homeostasis within skin depends on complex interactions between epidermal and underlying dermal layers, present study characterizes compares isolated fibroblasts (DFs) (Foxn1 deficient) wild-type Balb/c counterparts. Nude DFs exhibited a higher...
Since there are limited studies analyzing the impact of age, sex and obesity on cutaneous repair, current study evaluated excisional skin wound healing as a function diet in C57BL/6 mice subjected to either low (LFD) or high (HFD) fat diet.Older accumulated increased body relative younger under HFD.Skin at particular stages was affected by age aspect Tgfβ-1, MCP-1, Mmp-9 Mmp-13 expression.The most profound, cumulative effect observed for combination two parameters: sex.While males displayed...
FOXN1, a transcription factor expressed in the epidermis, regulates keratinocyte differentiation and participates skin wound healing. In this study, we explored impact of FOXN1 insufficiency on diet-stimulated weight gain dermal white adipose tissue regulation intact wounded FOXN1eGFP/+ (heterozygotes, FOXN1-insufficient) mice context age diet. The results showed that high-fat diet, gained significantly less body than their FOXN1+/+ counterparts (FOXN1-sufficient mice). displayed abrogated...
Aim: The transcription factor Foxn1 is a regulator of scar-ended cutaneous wound healing in mice. However, the link between and Wnt signaling has not been explored context repair. Here, we investigate effects β-catenin-dependent -independent represented by Wnt10a Wnt11, respectively, full-thickness wounds C57BL/6 Material Methods: Quantitative polymerase chain reaction, western blot, immunostaining were performed to assess spatial temporal distribution Wnt10a, β-catenin skin during healing....
Pressure injuries/ulcers are frequent complications in elderly, paraplegic, and quadriplegic patients, which account for considerable cost to the international health care economy remain refractory current treatment options. Autologous or allogeneic adult stromal/stem cells represent an alternative therapeutic approach. The study extends prior findings by exploring safety efficacy of human adipose-derived cell (ASC) therapy established immunocompetent murine skin pressure ulcer model where...
Dermal white adipose tissue (dWAT) is involved in the maintenance of skin homeostasis. However, studies concerning its molecular regulation are limited. In present paper, we ask whether introduction two transcription factors, Foxn1 and Hif-1α, into post-wounded Foxn1-/- mice regulates dWAT during wound healing (days 3 6). We have chosen lentivirus vectors (LVs) as a tool to deliver Hif-1α skin. documented that combinations both transgenes reduces number, size diameter dermal adipocytes at...
Skin exposed to environmental threats, including injuries and oxidative stress, develops an efficient but not fully recognized system of repair antioxidant protection. Here, using mass spectrometry analysis (LC–MS/MS), followed by in vitro vivo experiments, we provided evidence that Foxn1 keratinocytes regulates elements the electron transport chain participates thioredoxin (Txn2, Txnrd3, Srxn1) induction, particularly a hypoxic environment. We first showed upregulates glutathione reductase...
Abstract Human adipose-derived stem cells (ASCs) have potential to improve wound healing; however, their equivalents from domestic animals received less attention as an alternative cell-based therapy for or even humans. Hypoxia is essential maintaining cell functionality in tissue-specific niches. However, a cellular response low oxygen levels has not been demonstrated pig ASCs. Hence, the goal of our study was characterize ASCs isolated subcutaneous fat pigs (pASCs) and examine effect...
The primary mechanism by which adipose-derived stem cells (ASCs) exert their reparative or regenerative potential relies predominantly on paracrine action. Secretory abilities of ASCs have been found to be amplified hypoxia pre-conditioning. This study investigates the impact (1% O2) secretome composition pig (pASCs) and explores effect pASCs’ conditioned media (CM) skin cell functions in vitro expression markers attributed wound healing. Exposure pASCs increased levels vascular endothelial...
Hypoxia and hypoxia-regulated factors (eg, hypoxia-inducible factor-1α [Hif-1α], factor inhibiting Hif-1α [Fih-1], thioredoxin-1 [Trx-1], aryl hydrocarbon receptor nuclear translocator 2 [Arnt-2]) have essential roles in skin wound healing. Using Foxn1−/− mice that can heal injuries a unique scarless manner, we investigated the interaction between Foxn1 factors. The displayed impairments regulation of Hif-1α, Trx-1, Fih-1 but not Arnt-2 during healing process. An analysis wounded showed...
Pressure ulcers (PUs) result in part due to ischemia-reperfusion injury the skin and present frequently elderly or quadriplegic patients with reduced mobility. Despite high economic societal cost of this condition, PU therapy relies primarily on preventive strategies invasive surgical intervention. A growing body clinical literature suggests that localized injection adipose-derived cells can accelerate enhance closure PUs. The current study systematically evaluated safety human adipose...
The recognition of a distinct fat depot, the dermal white adipose tissue (dWAT), points out complexity interaction among skin resident cells: keratinocytes, fibroblasts (DFs) and adipocytes in response to physiological (diet, age) pathological (injury) stimulations. dWAT has been recognized as significant contributor thermoregulation, hair cycle, immune response, wound healing scarring. In this study, we examined age- diet-related changes modulation DFs’ adipogenic potential. data showed...
Adipose-derived stem cells (ASCs) from human and animal fat have emerged as therapeutic alternatives for damaged tissues. Pre-conditioning of ASCs with hypoxia results in their functional enhancement, which might facilitate the process healing. However, there is still a critical need large-scale preclinical studies to reinforce translation these findings into clinical practice humans veterinary medicine. Here, we adapted full-thickness excisional skin wound mouse model evaluate compare...
Abstract Intradermal adipocytes form dermal white adipose tissue (dWAT), a unique fat depot localized in the lower layer of dermis. However, recognition molecular factors regulating dWAT development, homeostasis, and bioactivity is limited. Using Foxn1 −/− +/+ mice, we demonstrated that epidermally expressed regulates development defines adipogenic capacity fibroblasts. In intact post‐wounded skin, contributes to initial stimulation adipogenesis participates modulation lipid metabolism...
Although the impact of age, gender, and obesity on skin wound healing process has been extensively studied, data related to gender differences in aspects scarring are limited. The present study performed abdominal human intact scar focused determining extracellular matrix (ECM) composition, dermal white adipose tissue (dWAT) accumulation, Foxn1 expression as a part response injury. Scar men showed highly increased levels COLLAGEN 1A1, 6A3, ELASTIN mRNA expression, accumulation thick collagen...
ABSTRACT Hypoxia and hypoxia-regulated factors [e. g., hypoxia-inducible factor-1α (Hif-1α), factor inhibiting Hif-1α (Fih-1), thioredoxin-1 (Trx-1), aryl hydrocarbon receptor nuclear translocator 2 (Arnt-2)] have essential roles in skin wound healing. Using Foxn1 −/− mice that can heal injuries a unique scarless manner, we investigated the interaction between factors. The displayed impairments regulation of Hif-1α, Trx-1 Fih-1 but not Arnt-2 during healing process. An analysis wounded...